Short-term effects of forest fire on the soil bacterial community-enzyme activity in typical forest stands in Jinyun Mountain, Chongqing
LAN Yue1,2, WANG Yunqi1,2, WANG Yujie1,2, CUI Xinrui1,2, ZHENG Yonglin1,2, SHEN Hang1,2, YAO Lu1,2, SI Hongtao3, LI Manyi3
1. Three-Gorges Reservoir Area(Chongqing) Forest Ecosystem Research Station, School of Soil and Water Conservation, Beijing Forestry University, 100083, Beijing, China; 2. Three-Gorges Reservoir Area(Chongqing) Forest Ecosystem Research Station, Ministry of Education, School of Soil and Water Conservation, Beijing Forestry University, 100083, Beijing, China; 3. Observation and Research Station of Ecological Restoration for Chongqing Typical Mining Areas, Ministry of Natural Resources, Chongqing Institute of Geology and Mineral Resources, 401120, Chongqing, China
Abstract：[Background] Forest fires are a driver of forest soil and biodiversity, especially in fire-prone ecosystems. The effects of fire on soil microbial communities as well as enzyme activity are complex and largely unknown. Therefore, analyzing the dynamics of soil bacteria and enzyme activities in the short term after fire is of great biological significance for understanding the reconstruction process of forest communities. [Methods] The soil of conifer and broad-leaved mixed forest and bamboo forest after the fire in Jinyun Mountain, Chongqing was used as the research object. The activities of sucrase, cellulase and urease as well as the changes of soil nutrients and pH were measured in the soil layers of 0-10 cm and 10-20 cm of conifer and broad-leaved mixed forest and bamboo forests under different fire intensities within one month of the fire, and the changes of soil bacterial community structure were analyzed by high-throughput sequencing technology. [Results] 1) The soil bacterial diversity, richness, soil organic matter and soil enzyme activities decreased in the short term after the occurrence of forest fires, and soil pH, available potassium, available nitrogen and available phosphorus increased to varying degrees. Especially in the 0-10 cm soil layer, it was more affected by forest fire. 2) The relative abundance of Proteomycetes, Actinomycetes and phylum Aerugula in the soil was the highest after moderate and severe forest fires. The relative abundance of Acidobacterium phylum was the highest when not exposed to forest fires. The relative abundance of Firmicutes was the highest after mild fire. 3) In the redundant analysis of soil bacterial structure and soil chemical factors, the changes of soil chemical properties after fire also affected soil bacterial communities and soil enzyme activities. [Conclusions] Forest fires can lead to a decrease in soil bacterial community diversity and a significant decrease in soil enzyme activity. The results indicate that moderate and severe forest fires have the greatest impact on soil bacterial diversity and enzyme activity, with a positive correlation between Proteobacteria and soil nutrients. Acidobacteriota demonstrates negative correlation with soil nutrients and pH value.
兰越, 王云琦, 王玉杰, 崔芯蕊, 郑永林, 申航, 姚璐, 司洪涛, 李满意. 林火对重庆缙云山典型林分土壤细菌群落及酶活性的短期影响[J]. 中国水土保持科学, 2023, 21(3): 60-68.
LAN Yue, WANG Yunqi, WANG Yujie, CUI Xinrui, ZHENG Yonglin, SHEN Hang, YAO Lu, SI Hongtao, LI Manyi. Short-term effects of forest fire on the soil bacterial community-enzyme activity in typical forest stands in Jinyun Mountain, Chongqing. SSWC, 2023, 21(3): 60-68.
张玉红,覃炳醒,孙铭隆,等.林火对大兴安岭典型林型林下植被与土壤的影响[J].北京林业大学学报,2012,34(2):7. ZHANG Yuhong, QIN Bingxing, SUN Minglong, et al. Impact of forest fire on understory vegetation and soil in typical forest types of Daxing'an Mountains, northeastern China [J]. Journal of Beijing Forestry University, 2012, 34(2):7.
[2]
BOWMAN D M J S, BALCH J K, ARTAXO P, et al. Fire in the Earth system [J]. Science, 2009, 324(5926):481.
[3]
舒立福,田晓瑞,李红.世界森林火灾状况综述[J].世界林业研究,1998(6):42. SHU Lifu, TIAN Xiaorui, LI Hong. Overview of the state of forest fires in the world [J]. World Forestry Research, 1998(6):42.
[4]
吴然,康峰峰,韩海荣,等.山西太岳山典型植被类型土壤微生物量特征[J].应用与环境生物学报,2016,22(3):486. WU Ran, KANG Fengfeng, HAN Hairong, et al. Soil microbial biomass properties under typical vegetation types in the Taiyue Mountain of China [J]. Chinese Journal of Applied and Environmental Biology, 2016, 22(3):486.
[5]
KNELMAN J E, GRAHAM E B, TRAHAN N A, et al. Fire severity shapes plant colonization effects on bacterial community structure, microbial biomass, and soil enzyme activity in secondary succession of a burned forest[J]. Soil Biology and Biochemistry, 2015, 90:161.
[6]
牛小云,孙晓梅,陈东升,等.日本落叶松人工林枯落物土壤酶活性[J].林业科学,2015,51(4):16. NIU Xiaoyun, SUN Xiaomei, CHEN Dongsheng, et al. Soil enzyme activities of the litter in Larix kaempferi plantation [J]. Scientia Silvae Sinicae, 2015, 51(4):16.
[7]
ENGELBRECHT C K,KATARINA I, BRANDSTRÖM D M, et al. Sample preparation for fungal community analysis by high-throughput sequencing of barcode amplicons [J]. Methods in Molecular Biology (Clifton, N.J.), 2016, 1399:61.
[8]
梁国鹏,HOUSSOU A A,吴会军,等.施氮量对夏玉米根际和非根际土壤酶活性及氮含量的影响[J].应用生态学报,2016,27(6):1917. LIANG Guopeng, HOUSSOU A A, WU Huijun, et al. Soil nitrogen content and enzyme activities in rhizosphere and non-rhizosphere of summer maize under different nitrogen application rates[J]. Chinese Journal of Applied Ecology, 2016, 27(6):1917.
[9]
刘冠宏,李炳怡,宫大鹏,等.林火对北京平谷区油松林土壤化学性质的影响[J].北京林业大学学报,2019,41(2):29. LIU Guanhong, LI Bingyi, GONG Dapeng, et al. Effects of forest fire on soil chemical properties of Pinus tabuliformis forest in Pinggu district, Beijing[J]. Journal of Beijing Forestry University, 2019, 41(2):29.
[10]
靳正忠,雷加强,徐新文,等.极端干旱区防护林地土壤微生物多样性[J].生态学报,2009,29(8):4548. JIN Zhengzhong, LEI Jiaqiang, XU Xinwen, et al. Microbial diversities of shelter-forest soils in the extreme arid area [J]. Acta Ecologica Sinica, 2009, 29(8):4548.
[11]
田荣,陈奇伯,黎建强,等.计划烧除对云南松林土壤微生物及酶活性的影响[J].生态环境学报,2020,29(4):695. TIAN Rong, CHEN Qibo, LI Jianqiang, et al. Effects of prescribed burning on soil microbes and enzyme activities in Pinus yunnanensis forests[J]. Ecology and Environmental Sciences, 2020, 29(4):695.
[12]
ADKINS J, DOCHERTY K M, GUTKNECHT J L M, et al. How do soil microbial communities respond to fire in the intermediate term? Investigating direct and indirect effects associated with fire occurrence and burn severity[J]. Science of the Total Environment,2020,745:140957.
[13]
WOOLET J, WHITMAN T. Pyrogenic organic matter effects on soil bacterial community composition[J]. Soil Biology and Biochemistry, 2020, 141(C):107678.
[14]
张平究,梁川,陈芳,等.退耕还湿后土壤细菌群落结构和生物量变化过程研究[J/OL].生态学报,2023(11):1. ZHANG Pingjiu, LIANG Chuan, CHEN Fang, et al. Changes of soil bacterial community structures and biomass in wetlands recovered from farmland[J/OL]. Acta Ecologica Sinica, 2023(11):1.
[15]
赵彬,孙龙,胡海清,等.兴安落叶松林火后对土壤养分和土壤微生物生物量的影响[J].自然资源学报,2011,26(3):450. ZHAO Bin, SUN Long, HU Haiqing, et al. Post-fire soil microbial biomass and nutrient content of Larix gmelinii forest in Autumn [J]. Journal of Natural Resources, 2011, 26(3):450.
[16]
GONZÁLEZ-PÉREZ J A, GONZÁLEZ-VILA F J, ALMENDROS G, et al. The effect of fire on soil organic matter: A review[J]. Environment International, 2004, 30(6):855.
[17]
王玉哲,刘俊第,严强,等.马尾松林采伐迹地火烧黑炭对土壤活性碳氮库的影响[J].生态学报,2018,38(20):7198. WANG Yuzhe, LIU Jundi, YAN Qiang, et al. Effects of black carbon on soil labile carbon and nitrogen pools in a Pinus massoniana plantation subjected to slash burning[J]. Acta Ecologica Sinica, 2018, 38(20):7198.
[18]
谷会岩,金靖博,陈祥伟,等.不同火烧强度林火对大兴安岭北坡兴安落叶松林土壤化学性质的长期影响[J].自然资源学报,2010,25(7):1114. GU Huiyan, JIN Jingbo, CHEN Xiangwei, et al. The long-term impacts on chemical properties of Larix gmelini forest on the norther slope of Greater Hinggan Mountains from a forest fire of varying fire intensity[J]. Journal of Natural Resources, 2010, 25(7):1114.
[19]
HERNÁNDEZ T, GARCÍA C, REINHARDT I. Short-term effect of wildfire on the chemical, biochemicaland microbiological properties of Mediterranean pine forest soils[J]. Biology and Fertility of Soils, 1997, 25(2):109.
[20]
MARÍ D L P J, ANA D L H, LAURA P, et al. Soil quality: A new index based on microbiological and biochemical parameters[J]. Biology and Fertility of Soils, 2002, 35(4):302.
2023, Vol. 21 
