Response of biomass and antioxidant enzyme activities of Gymnocarpos przewalskii seedlings to drought stress
CUI Peng1, HUANG Haixia1, YANG Qiqi2
1. College of Forestry, Gansu Agricultural University, 730070, Lanzhou, China; 2. Gansu Vocational and Technical College of Forestry, 741020, Tianshui, Gansu, China
Abstract：[Background] Drought stress affects the biomass accumulation and distribution between the aboveground and roots, results in excessive accumulation of active oxygen and lipid peroxidation, while plants evolve antioxidant enzyme system. Gymnocarpos przewalskii originates from Tethys, is the rare relic species of the Tertiary period in the desert area of central Asia and plays an important role in keeping stable of desert ecosystem. The research is to analyze the biomass accumulation and distribution pattern of the seedlings, the antioxidant characteristics of the leaves and roots under drought stress for revealing the drought resistance mechanism and to provide some theoretical basis for rare species conservation and vegetation restoration in desert area. [Methods] Taking one-year-old seedlings of Gymnocarpos przewalskii as the experiment material and using pot experiment, four water gradients were set, including the control, light water stress, moderate water stress and severe water stress, the soil water content was kept at 45%-50%, 30%-35%, 15%-20% and 5%-10% of the field capacity respectively by weighing method, with 10 repetitions per pot experiment, leaves and roots were collected after being treated 90 d and the biomass and physiological indexes were measured. The difference in biomass, root-shoot ratio, malondialdehyde (MDA) content and antioxidant enzyme activities in leaves and roots was analyzed. [Results] Under drought stress, biomass of the whole plant and the aboveground significantly reduced, the underground biomass decreased remarkably under the moderate and severe stress, and the aboveground biomass decreased more, thus the root-shoot ratio increased and was significantly higher under the severe stress compared to the control. MDA content in roots and leaves increased remarkably under drought stress compared with the control, the higher the drought stress degree was, the more serious the peroxidation damage was, and the increase amplitude of MDA content in roots was higher than that in leaves under the same treatment. With the increase of the drought stress, superoxide dismutase (SOD) activity in leaves and roots first significantly increased then decreased, it was obviously higher than the control in roots but significantly lower in leaves under severe stress, which indicated that the scavenging effect of SOD to reactive oxygen was limited especially in leaves. Peroxidase (POD) activity markedly decreased in roots but significantly increased in leaves under stress treatments compared to the control, showing that POD played obvious antioxidation in leaves, and POD was not the key enzyme scavenging H2O2 in the roots under drought stress. Catalase (CAT) activity increased significantly in leaves and roots under drought stress. Ascorbate peroxidase (APX) activity showed increasing trend under stress treatment, the difference was significant among the treatments in roots, and it changed markedly under moderate and severe stress in leaves. [Conculsions] Drought stress clearly affected the growth of Gymnocarpos przewalskii seedlings, but it could increase biomass distribution to roots to adapt the drought. Drought stress caused obvious peroxidation damage to the leaf and root membrane system, and the damage was more serious in roots. The leaves protected the membrane system by synergistic action of SOD, CAT, POD and APX enzymes, and the roots mainly reduced damage of reactive oxygen by SOD, CAT and APX.
韩一林. 毛竹对干旱、高温及协同胁迫的生理生化响应[D].北京:中国林业科学研究院,2017:2. HAN Yilin. Physiological and biochemical responses of Phyllostachys edulis to drought, heat and synergistic stress[D].Beijing:Chinese Academy of Forestry.2017:2
[2]
杨建伟,孙桂芳,赵丹,等.干旱胁迫对3种灌木生长及叶水分生理的影响[J].林业与生态科学,2018,33(4):423. YANG Jianwei, SUN Guifang, ZHAO Dan, et al.Effects of drought stress on the growth of three shrubs and their leaf water physiology[J].Forestry and Ecological Sciences,2018,33(4):423.
[3]
单立山. 西北典型荒漠植物根系形态结构和功能及抗旱生理研究[D].兰州:甘肃农业大学,2013:4. SHAN Lishan. Study onroot morphological structure, function and drought resistance physiology of typical desert plants in northwest China[D].Lanzhou:Gansu Agricultural University,2013:4.
[4]
李洁,周春娥,梁志英,等.干旱胁迫对乌头部分生理指标的影响[J].中国水土保持科学,2011,9(3):93. LI Jie, ZHOU Chune, LIANG Zhiying, et al. Impacts of drought stress on physiological characteristics of Aconitums[J]. Science of Soil and Water Conservation,2011,9(3):93.
[5]
曹昀,纪欣圣,国志昌,等.土壤水分含量对虉草幼苗保护酶与渗透调节物质的影响[J].干旱区地理,2018,41(4):780. CAO Yun, JI Xinshenng, GUO Zhichang, et al. Effects of soil moisture content on antioxidase and osmoregulation substrate content of Phalaris arundinacea seedlings[J].Arid Land Geography,2018,41(4):780.
[6]
白娟,龚春梅,王刚,等.干旱胁迫下荒漠植物红砂叶片抗氧化特性[J].西北植物学报,2010,30(12):2444. BAI Juan, GONG Chunmei, WANG Gang, et al. Antioxidative characteristics of Reaumuria soongorica under drought stress[J].Acta Botanica Boreali-Occidentalia Sinica, 2010,30(12):2444.
[7]
SINHABABU A, KAR R K. Comparative responses of three fuel wood yielding plants to PEG-induced water stress at seedling stage[J]. Acta Physiologiae Plantarum, 2003, 25(4):403.
[8]
郭艳阳,刘佳,朱亚利,等.玉米叶片光合和抗氧化酶活性对干旱胁迫的响应[J].植物生理学报,2018,54(12):1839. GUO Yanyang, LIU Jia, ZHU Yali, et al. Responses of photosynthetic and antioxidant enzyme activities in maize leaves to drought stress[J]. Plant Physiology Journal,2018,54(12):1839.
[9]
姜宗庆,李成忠,余乐,等.干旱胁迫对薄壳山核桃叶片丙二醛含量和3种抗氧化酶活性的影响[J].上海农业学报,2019,35(1):7. JIANG Zongqing, LI Chengzhong,YU Le, et al. Effects of drought stress on MDA content and 3 antioxidant enzymes activities in leaves of Carya illinoensis[J].Acta Agriculturae Shanghai,2019,35(1):7.
[10]
殷东生,魏晓慧. 干旱胁迫对风箱果幼苗生长、光合生理和抗氧化酶活性的影响[J].东北林业大学学报,2019,47(1):26. YIN Dongsheng, WEI Xiaohui. Influence of drought stress on growth, photosynthetic physiology and antioxidant enzyme activities of Physocarpus amurensis seedlings[J]. Journal of Northeast Forestry University,2019,47(1):26.
[11]
傅立国.中国植物红皮书[M].北京:科学出版社,1992:368. FU Liguo. China plant red data book[M]. Beijing:Science Press, 1992:368.
[12]
马松梅,张明理,张宏祥,等.利用最大熵模型和规则集遗传算法模型预测孑遗植物裸果木的潜在地理分布及格局[J].植物生态学报,2010,34(11):1327. MA Songmei, ZHANG Mingli, ZHANG Hongxiang, et al.Predicting potential geographical distributions and patterns of the relic plant Gymnocarpos przewalskii using maximum entropy and genetic algorithm for rule-set prediction[J]. Chinese Journal of Plant Ecology, 2010, 34(11):1327.
[13]
王立龙,王亮,张丽芳,等.不同生境下濒危植物裸果木种群结构及动态特征[J].植物生态学报,2015,39(10):980. WANG Lilong, WANG Liang, ZHAG Lifang, et al. Structure and dynamic characteristics of Gymnocarpos przewalskii in different habitats[J].Chinese Journal of Plant Ecology,2015,39(10):980.
[14]
连转红. 裸果木叶片对自然干旱的生理响应[D].兰州:甘肃农业大学,2018:9. LIAN Zhuanhong. Physiological response of Gymnocarpos przewalskii leaf to natural drought[D].Lanzhou:Gansu Agricultural University,2018:9.
汪攀,陈奶莲,邹显花,等. 植物根系解剖结构对逆境胁迫响应的研究进展[J].生态学杂志,2015,34(2):550. WANG Pan, CHEN Nailian, ZOU Xianhua, et al. Advances in research on response of plant root anatomy to stress[J].Journal ofEcology,2015,34(2):550.
[18]
单立山,李毅,石万里,等.土壤水分胁迫对红砂幼苗生长和渗透调节物质的影响[J].水土保持通报,2015,35(6):106. SHAN Lishan, LI Yi, SHI Wanli, et al. Effects ofsoil water stress on growth and osmotic adjustment substances of Reaumuria songarica seedlings[J]. Soil and Water Conservation Bulletin,2015,35(6):106.
[19]
李冬梅,焦峰,雷波,等.黄土丘陵区不同草本群落生物量与土壤水分的特征分析[J].中国水土保持科学,2014,12(1):33. LI Dongmei,JIAO Feng,LEI Bo, et al. Characteristics of biomass and soil moisture of different herb communities in loess hilly region[J]. Science of Soil and Water Conservation,2014,12(1):33.
[20]
李芳兰,包维楷,吴宁.白刺花幼苗对不同强度干旱胁迫的形态与生理响应[J].生态学报,2009,29(10):5406. LI Fanglan, BAO Weikai, WU Ning. Morphological and physiological responses of Sophora davidii seedlings to different stress drought stress[J]. Acta Ecologica Sinica,2009,29(10):5406.
[21]
ZHANG Shize, HUA Baozhen, ZHANG Fan. Induction of the activities of antioxidative enzymes and the levels of malondialdehyde in cucumber seedlings as a consequence of Bemisia tabaci(Hemiptera:Aleyrodidae) infestation[J]. Arthropod-Plant Interactions, 2008, 2(4):209.
[22]
罗青红,宁虎森,何苗,等.5种沙地灌木对干旱胁迫的生理生态响应[J].林业科学,2017,53(11):29. LUO Qinghong, NING Husen, HE Miao, et al. Ecophysiological responses of five sandy shrubs to drought stress[J].Scientia Silvae Sinicae,2017,53(11):29.
[23]
莫荣利,李勇,于翠,等.水分胁迫对桑树生理生化特性的影响[J].湖北农业科学,2017,56(24):4815. MO Rongli, LI Yong, YU Cui, et al. Effect of water stress on physiological-biochemical characteristics of Morus alba[J].Hubei Agricultural Sciences,2017,56(24):4815.
[24]
种培芳,李航逸,李毅.荒漠植物红砂根系对干旱胁迫的生理响应[J].草业学报,2015,24(1):72. CHONG Peifang, LI Hangyi, LI Yi. Physiological responses of seeding roots of the desert plant Reaumuria soongorica to drought stress[J].Acta Prataculturae Sinica,2015,24(1):72.
[25]
庄国庆,王文国,王胜华,等.干旱胁迫下麻疯树幼苗根和叶的生理应答变化[J].四川大学学报(自然科学版),2009,46(6):1845. ZHUANG Guoqing, WANG Wenguo, WANG Shenghua, et al. Different physiological response to drought stress in root and leaf of seedlings of Jatropha cureas L.[J].Journal of Sichuan University(Natural Science Edition),2009,46(6):1845.
[26]
SOFO A, DIHIO B, XILOYANNIS C, et al. Antioxidant defenses in olive trees during drought stress:Changes in activity of some antioxidant enzymes[J]. Functional Plant Biology, 2005, 32(1):45.
[27]
韩刚,党青,赵忠.干旱胁迫下沙生灌木花棒的抗氧化保护响应研究[J].西北植物学报,2008(5):1007. HAN Gang, DANG Qing, ZHAO Zhong. Response of antioxidation protection system of Hedysarum scoparium to drought stress[J].Acta Botanica Boreali-Occidentalia Sinica,2008(5):1007.
[28]
吴淼,刘信宝,丁立人,等.PEG模拟干旱胁迫下硅对紫花苜蓿萌发及生理特性的影响[J].草地学报,2017,25(6):1258. WU Miao, LIU Xinbao, DING Liren, et al. Effects of silicon on germination and physiological characteristics of Medicago sativa under drought stress simulated by PEG[J].Acta Agrestia Sinica,2017,25(6):1258.
[29]
尹永强,胡建斌,邓明军.植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J].中国农学通报, 2007,23(1):105. YIN Yongqiang, HU Jianbin, DENG Mingjun. Latest development of antioxidant system and responses to stress in plant leaves[J].Chinese Agricultural Science Bulletin, 2007,23(1):105.
[30]
NIINOMI A, MORIMOTO M, SHIMIZU S. Lipid peroxidation by the[peroxidase/H2O2/phenolic]system[J].Plant and Cell Physiology, 1987,28(4):731.
[31]
卜楠,俞丽蓉,马万里,等.不同水分条件下沙漠豆生理指标的变化[J].中国水土保持科学,2012,10(6):77. BU Nan, YU Lirong, MA Wanli, et al. Physiological changes of Swainsona formosa under different water conditions[J].Science of Soil and Water Conservation,2012,10(6):77.
[32]
田艳.干旱胁迫下光核桃根系响应机制初步研究[D].哈尔滨:东北林业大学,2016:2. TIAN Yan. Theresponding mechanism of physiology and proteomics in Prunus mira roots under drought[D].Harbin:Northeast Forestry University,2016:2.
2020, Vol. 18 
