Microbial prevention and control is an essential task for the protection of paper archives. However, the harmful conservation process to human body, as well as potential contamination to the environment, has always been troubling the workers. In this paper, non-harmful Eco-friendly bacteriostatic agent is selected to carry out bacteriostatic experiments on paper-based documents. Firstly, the bacteriostatic agents are selected based on antibacterial activity. Among contact-type bacteriostatic agent, their effectiveness can be ranked as pimamycin > PHMB > Epsilon-PL > Allicine; the effectiveness of spatial bacteriostatic agent can be ranked as carvol > Citronellal > 2-phenylethanol. Secondly, the test for synergistic bacteriostatic effect is carried out. PHMB (125mg/L) and Pimamycin (3.906mg/L) forms the contact-type synergistic pair that produces the optimal bacteriostatic effect. Carvol (15.62mg/L) and Citronellol (31.25mg/L) forms the spatial-type synergistic pair that has the best bacteriostatic performance. Finally, the applicability of the two identified synergistic bacteriostatis pairs were tested. Results show that both pairs of bacteriostatis agents did not induce significant changes to the tensile force of paper; significant diacidification and anti-aging effects are observed; the value of ΔE should not exceed 1.5 after the treatment. The identification, selection and validation process could provide practical basis for future development of safe and efficient bacteriostatic agent for paper.