Effects of drought stress and rewatering on physiological characteristics of Arundo donax var. versicolor
XU Lingming1, CAO Yun1, TANG Siwen1, LU Yuanhong1, LUO Shanshan1, MA Yinshan2
1. School of Geography and Environment, Jiangxi Normal University/Key Laboratory of Poyang Lake Wetland and Watershed Research Ministry of Education, Jiangxi Normal University, 330022, Nanchang, China; 2. College of Agriculture and Biotechnology, Hexi University, 734000, Zhangye, Gansu, China
Abstract：[Background] Moisture is the main component of plants, affecting the growth, development, metabolism and geographical distribution of plants. Recently, plant physiological ecology research mainly focuses on drought stress, photosynthesis, chlorophyll fluorescence, antioxidant system and osmotic adjustment substances. The effects of drought stress and rewatering on the physiological characteristics of Arundo donax var. versicolor were studied in order to provide reference for the introduction, cultivation and management of wetland gardens and to provide more choices for the use of wetland plants for soil and water conservation in wetland parks.[Methods] Taking the A. donax var. versicolor seedlings as experimental materials, the effects of drought stress and rewatering on the physiological characteristics of A. donax var. versicolor were studied. The indoor pot experiment was conducted to set 9 water gradients[CK:(53±0.5)%(Soil water saturation), C1:(50±0.5)%, C2:(45±0.5)%, C3:(40±0.5)%, C4:(35±0.5)%, C5:(30±0.5)%, C6:(20±0.5)%, C7:(10±0.5)%, C8:(5±0.5)%]. The chlorophyll content, carotenoid content, antioxidant enzyme activity, soluble protein content, malondialdehyde content, and root activity of A. donax var. versicolor were determined, and the effects of different stress treatments on its physiology were analyzed by SPSS 21.0、Microsoft-Excel 2010 and One-wayANOVA.[Results] 1) With the prolongation of stress time, the chlorophyll content of A. donax var. versicolor leaves decreased with the degree of stress deepening, the content of carotenoids decreased first and then increased during the 40%-50% soil moisture content with the prolongation of stress time, while the control and other treatment groups showed a decreased trend. 2) With the prolongation of stress time, the activity of SOD increased first and then decreased, the activity of POD decreased first and then increased, and the activity of CAT increased. With the degree of stress deepening, the activity of SOD increased, while the activities of POD and CAT increased first and then decreased. 3) With the prolongation of stress time, the content of soluble protein and MDA increased first and then decreased, and both increased with the increase of stress degree. 4) After rewatering, the photosynthetic pigments of A. donax var. versicolor leaves increased, and were 40%-50% higher than control groups. The activities of antioxidant enzymes decreased, the activity of SOD was not significantly different from that in the control group, while the activity of POD was significantly different from the control group, and the activities of CAT was extremely different from the control group. The contents of soluble protein and MDA decreased. and the root activity was significantly higher than rewatering before.[Conclusions] Under drought stress, the photosynthetic pigment synthesis of A. donax var. versicolor leaves were inhibited, but it could respond to oxidative damage by coordinating antioxidant enzymes to scavenge free radicals and accumulating soluble proteins. After rewatering, all indicators could be restored to the control level, showing a certain drought tolerance.
许令明, 曹昀, 汤思文, 陆远鸿, 罗姗姗, 马银山. 干旱胁迫及复水对花叶芦竹生理特性的影响[J]. 中国水土保持科学, 2020, 18(3): 59-66.
XU Lingming, CAO Yun, TANG Siwen, LU Yuanhong, LUO Shanshan, MA Yinshan. Effects of drought stress and rewatering on physiological characteristics of Arundo donax var. versicolor. SSWC, 2020, 18(3): 59-66.
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2020, Vol. 18 
