Abstract：[Background] It researched the effect of ectomycorrhizal fungi on Pinus tabulaeformis seedlings to increase the survival rate of seedlings, promote the regeneration of tree species, and prevent soil erosion.[Methods] Potted water was used to simulate drought stress, and four water treatments were designed:control(CK),light drought(LD),moderate drought(MD),severe drought(SD). Inoculating the seedlings of Pinus tabulaeformis with Trichoderma spp. was to study the effect of growth which ectomycorrhizal fungi had on P. tabulaeformis seedling under drought stress. To each treatment,It selected seedlings whose growth was basically the same, and measured the morphological and physiological indexes. The specific operation refered to Principles and Techniques of Plant Physiological and Biochemical Experiments written by Li Hesheng.[Results] The plant height and needle length of P. tabulaeformis seedlings were significantly affected by inoculation with Trichoderma spp. and drought stress treatment (P<0.01), but the interaction was not significant (P>0.05).The effects of inoculation with Trichoderma spp. and drought stress on the soluble protein, soluble sugar and starch content of P. tabulaeformis seedlings were significant (P<0.01). However, the interaction to the soluble protein and starch content of Pi. tabulaeformis seedlings was not significant (P>0.05), and the interaction to the soluble sugar content was significantly affected (P<0.01). The detailed analysis results to the morphological and physiological indexes of P. tabulaeformis seedlings were as follows:(1) After inoculation with Trichoderma spp., the biomass and root-shoot ratio of P. tabulaeformis seedlings were significantly increased (P<0.05).The mycorrhizal dependence on Trichoderma spp. was moderately dependent. Under drought stress, the plant height and needle length of P. tabulaeformis seedlings showed a decreasing trend. However, after inoculation with Trichoderma spp., the decrease of plant height and needle length was weakened, and the drought resistance of seedling was significantly enhanced (P<0.01). (2) With the increase of drought stress, the soluble protein and starch content of needles in P. tabulaeformis seedlings continued to decrease. After inoculation with Trichoderma spp., the corresponding index showed a decreasing trend with increasing stress, but the trend of inoculated Trichoderma spp. seedlings was weaker than that of uninoculated seedlings. (P<0.01). (3) Under drought stress, the soluble sugar content of needles in P. tabulaeformis seedlings showed a trend of "first rise and then fall". The soluble sugar content of seedlings uninoculated and inoculated Trichoderma spp. reached the highest value in LD and MD, respectively. In other words, the drought tolerance threshold of the seedlings increased.[Conclusions] The results showed that under drought stress, ectomycorrhizal fungi promoted the growth of P. tabulaeformis seedlings. Under moderate drought stress, Trichoderma spp. had the most significant effect on the growth of P. tabulaeformis seedlings. Under the same water treatment, the mycorrhizal seedlings had stronger drought resistance than non-mycorrhizal seedlings.
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2019, Vol. 17 
