Abstract:In the rocky desertification area of Guizhou Province, according to the distribution of carbonate rock, landform and rock types, a total of 152 root habitat profiles were surveyed. On the basis of rock type, occurrence, the number of patches of rock lithology within the range of root distribution, soil depth, crack width, vertical range of soil distribution, length and width of the root distribution, the types and characteristics of root growth space were analyzed by using RDA sorting method; Using a combination of qualitative and quantitative analysis method, a fast clustering was conducted, plant root habitat types were classified and named, feature of habitat types was described. The results showed that: 1) the plant root growth space in rocky desertification areas was divided into surface space, single-layer space, multilayer space; on multilayer space was used mostly plant root; 2) Root habitat of Guizhou karst rocky desertification area was divided into 18 types, features of various types of root habitat were different. Results of this study deepen the understanding of the habitats of plant root in Karst rocky desertification area. It is of great significance for the ecological restoration and rocky desertification control.