Abstract: 【Purpose/significance】Based on the perspective of information ecology, research the dynamic evolution of the network pub⁃ lic opinion ecology under the subject-object-time and space environment, and promote the ecological development of network public opinion through different dimensions of network public opinion culture leading methods and mechanisms, and provide new ideas for the supervision and governance of network public opinion Ideas to promote the ecological construction of network culture.【Method/ process】Describe the dynamic balance of the relationship between the subject, the object, the space-time environment and the inter⁃ action of the network public opinion, and build a panoramic model of the network public opinion ecology. According to the research di⁃ mension of the ecology of online public opinion, the cultural guidance dimension of subject guidance, object guidance and space-time environment guidance is proposed, as well as the corresponding cultural guidance mechanism.【Result/conclusion】Aiming at the three dimensions of subject-object-time and space environment, the cultural education mechanism, cultural service mechanism and cultural innovation mechanism are respectively proposed, which are of guiding significance for the governance of network public opin⁃ ion and the construction of network culture ecology.【Innovation/limitation】Innovatively combine the ecology of online public opinion and cultural guidance from the subject-object-temporal and spatial environment, and promote the governance of online public opinion and the construction of cultural ecology.