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| Effect of nitrogen addition on enzyme activity during fine root decomposition of Pinus tabulaeformis |
| MENG Min1, JING Hang1, YAO Xu1, WANG Guoliang1,2, LIU Guobin1,2 |
1. Institute of Soil and Water Conservation, Northwest A & F University, 712100, Yangling, Shaanxi, China;
2. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, 712100, Yangling, Shaanxi, China |
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Abstract [Background] Exploring the effect of nitrogen (N) addition on enzyme activity during fine root decomposition is helpful to elucidate the potential mechanism of N addition affecting fine root decomposition, and eventually provides a basis for studying N deposition affecting carbon balance and nutrient cycling in forest ecosystem.[Methods] The Pinus tabulaeformis seedling were treated with N addition (N0, 0 g/(m2·a); N3, 3 g/(m2·a); N6, 6 g/(m2·a); and N9, 9 g/(m2·a)) for 2 years, and then the fine roots were grouped with 3 diameter size classes (< 0.5 mm; 0.5-1.0 mm and 1.0-2.0 mm) and buried using litterbag method in a P. tabulaeformis forest where was treated with N addition for 2 years, respectively. The litterbags were sampled every 2 months, then the enzyme activities (cellobiase, alkaline phosphatase and galactosidase) and carbon (C), N phosphorus (P) concentrations were determined in laboratory.[Results] During fine root decomposition, the activity of cellobiase increased first and then decreased, and then increased in the later stage. The activities of alkaline phosphatase and galactosidase increased first and then decreased, and the activities were the highest in the middle stage of fine root decomposition. N addition significantly affected cellobiase and galactosidase activities (P< 0.05), but had no significant effect on alkaline phosphatase activity. N3 treatment increased cellobiase activity of 0-0.5 mm and >0.5-1.0 mm fine roots, N9 treatment increased cellobiase activity of >1.0-2.0 mm fine root. N addition decreased galactosidase activity of 0-0.5 mm fine root, and N9 decreased galactosidase activity of >0.5-1.0 mm and >1.0-2.0 mm fine roots. At different stages of decomposition, the effect of N addition on enzyme activity varied depending on the enzyme species and fine root diameters. There were significant positive correlations among different enzyme activities, and the correlation between alkaline phosphatase activity and galactosidase activity was the strongest. Correlation analysis showed that enzymes not only had specific influences, but also had common relations among enzyme species. Enzyme activities were significantly positively correlated with fine root C, N, P concentrations, and negatively correlated with fine root N:P ratio. The correlations between enzyme activity and fine root quality were relatively consistent, but the correlations between enzyme activity and temperature, moisture were quite different.[Conclusions] N addition has a variety of effects on the enzyme activity, such as increasing and decreasing, there is an interaction among N addition, decomposition time and fine root diameter to make the effect of N addition on enzyme activity varied during fine root decomposition. N addition increases decomposition enzyme activity by increasing fine root quality, environmental conditions such as temperature and moisture has varied effects on different enzyme activities during this process.
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Received: 04 November 2018
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