NaCl或PEG胁迫对裸果木种子萌发的影响

doi:10.16843/j.sswc.2017.06.011

Abstract
Figure/Table
References (0)
Related Citation (4)

Download: PDF (1429 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract [Background] Gymnocarpos przewalskii is the rare relic of tertiary and endangered species of desert area in central Asia, and one of the oldest groups in saline environment. It is mainly distributed in the desert area in our country being one of the main constructive species in stony desert area where rainfall is small but evaporation is strong, which results in the salt accumulation in soil, subsequently drought and soil salt affect its seed germination and further its population establishment and regeneration. To protect G. przewalskii, salt and drought stress was respectively stimulated to research salt and drought resistance mechanism in seed germination.[Methods] Seeds of G. przewalskii were collected from botanic garden of Anxi Extra-arid Desert National Nature Reserve with the mean annual rainfall of 45.3 mm and the salt content more than 2% in the soil depth of 30 cm. Using the different concentration of NaCl (50,100, 150, 200, 250, and 300 mmol/L) and the different potential of PEG-6000(-0.1, -0.3, -0.5, -0.7, and -0.9 MPa) to stimulate salt stress and drought stress respectively, taking the distilled water as the control to measure the germinated seeds and time, germination rate, germination energy, germination index vigor index and average germination days were calculated and the germination characteristics and the response to salt stress and drought stress were analyzed.[Results] The results showed that the germination rate and the vigor index increased at low concentration of NaCl (50 mmol/L) compared with the control. The germination rate, germination energy and average germination days were not significantly affected at the concentration of 100 and 150 mmol/L NaCl, and the germination rate, germination energy and vigor index decreased insignificantly at the osmotic potential of -0.3 MPa, which indicated that germination ability and uniformity was not significantly affected by relatively low concentration of NaCl (100 and 150 mmol/L) or relatively high osmotic potential of PEG (≥ -0.3 MPa). At high concentration of NaCl (≥ 250 mmol/L) or low osmotic potential of PEG (≤ -0.7 MPa), the germination rate, germination energy, germination index and vigor index extremely significantly decreased and the germination process was markedly prolonged, the seed germination of G. przewalskii was significantly inhibited and delayed due to the intensified osmosis and toxic action. Regression analysis indicated that the critical and limited value of NaCl stress on seed germination was 337.4 mmol/L and 628.6 mmol/L, respectively and the critical and limited value of PEG stress on seed germination was -0.70 MPa and -1.01 MPa, respectively.[Conculsions] The seed germination of G. przewalskii was improved at the concentration of 50 mmol/L NaCl and showed some tolerance to salt and drought stress, beyond the range (more than NaCl concentration of 250 mmol/L or less than PEG osmotic potential of -0.7 MPa), its seed germination was significantly inhibited. Researching the seed germination characteristics of G. przewalskii can provide some basis for the cultivation of G. przewalskii and combating desertification in desert area.

Key words： Gymnocarpos przewalskii NaCl stress PEG stress seed germination

Received: 06 March 2017

PACS:

S157

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LIAN Zhuanhong
	YANG Haili
	WU Fangming
	HUANG Haixia

Cite this article:

LIAN Zhuanhong,YANG Haili,WU Fangming, et al. Effect of NaCl or PEG stress on seed germination of Gymnocarpos przewalskii[J]. SSWC, 2017, 15(6): 89-96.

URL:

http://journal12.magtechjournal.com/Jweb_stbc/EN/10.16843/j.sswc.2017.06.011 OR http://journal12.magtechjournal.com/Jweb_stbc/EN/Y2017/V15/I6/89

[1]	王生军,刘果厚.内蒙古灌木资源[M].呼和浩特:内蒙古大学出版社, 2006:1. WANG Shengjun, LIU Guohou.Shrub resources in Inner Mongolia[M]. Hohehot:Inner Mongolia University Press,2006:1.
[2]	朱晓梅.裸果木(Gymnocarpos przewalskii)、四合木(Tetraena mongolica)、革包菊(Tugarinovia mongolica)三种荒漠植物起源地及迁移路线研究[D]. 呼和浩特:内蒙古农业大学, 2008:2. ZHU Xiaomei.Study on origin and migration of three desert plants:Gymoncarpos przewalskii, Tetraena mongolica, Tugarinaovia mongolica[D]. Hohehot:Inner Mongolia Agricultural University,2008:2.
[3]	刘家琼, 蒲锦春. 我国沙漠中部地区主要不同生态类型的植物的水分关系和旱生结构比较研究[J]. 植物学报, 1987, 29(6):662. LIU Jiaqiong, PU Jinchun. Comparative studies on water relations and xeromorphic structures of some plant species in the middle part of the desert zone in China[J]. Journal of Integrative Plant Biology,1987, 29(6):662.
[4]	柴永青,曹致中,蔡卓山,等.肃北地区稀有植物裸果木种群的空间分布格局[J]. 草业学报, 2010, 19(5):239. CHAI Yongqing,CAO Zhizhong,CAI Zhuoshan,et al. The spatial distribution patterns of rare plant Gymnocarpos przewalskii communities in Subei[J]. Acta Prataculturae Sinica, 2010, 19(5):239.
[5]	巴哈尔古丽,汪志军,郭中军,等.珍稀濒危植物裸果木地理分布与资源现状[J].中国野生植物资源,2005,24(5):39. Bahaerguli,WANG Zhijun, GUO Zhongjun, et al. Geographic distribution and current status of resources of rare endangered plant Gymnocarpos przewalskii[J].Chinese Wild Plant Resources.2005, 24(5):39.
[6]	汪之波,高清祥.珍稀濒危植物裸果木生物学特性研究[J].资源开发与市场,2009, 25(6):481. WANG Zhibo, GAO Qingxiang. Study on biological characteristics of rare endangered plant Gymnocarpos Przewalskii [J].Resource Development & Market, 2009, 25(6):481.
[7]	马松梅,张明理,张宏祥,等.利用最大熵模型和规则集遗传算法模型预测孑遗植物裸果木的潜在地理分布及格局[J].植物生态学报,2010,34(11):1327. MA Songmei, ZHANG Mingli, ZHANG Hongxiang, et al. Predicting potential geographical distribution sand patterns of the relic plant Gymnocarpos przewalskii using maximum entropy and genetic algorithm for rule-set prediction[J].Chinese Journal of Plant Ecology,2010,34(11):1327.
[8]	杨赵平,梁继业,柴学平,等.环境因子对珍稀濒危植物裸果木种子萌发特性的影响[J].西南林业大学学报,2017,37(1):49. YANG Zhaoping,LIANG Jiye,CHAI Xueping,et al. Influence of environmental factors on seeds germination of endangered plant Gymnocarpos przewalskii[J]. Journal of Southwest Forestry University, 2017, 37(1):49.
[9]	唐欣. 荒漠孑遗植物裸果木繁育系统及其传粉特性[D].乌鲁木齐:新疆农业大学,2016:48. TANG Xin. The breeding system and pollination characteristics of Gymnocarpos przewalskii Maxim[D]. Urumqi:Xinjiang Agricultural University, 2016:48.
[10]	李娟.珍稀濒危植物裸果木群落物种多样性及其形态解剖学研究[D].山西临汾:山西师范大学, 2012:6. LI Juan. The studies on species diversity and morphology and anatomy of the rare endangered plants of Gymnocarpos przewalskii Maxim[D]. Linfen in Shanxi:Shanxi Normal University, 2012:6.
[11]	王之波,高详清,孙继周.稀有植物裸果木的组织培养及植株再生[J]. 西北植物学报,2004,24(7):1319. WANG Zhibo, GAO Xiangqing, SUN Jizhou. Tissue culture and plant of rare plant Gymnocarpos przewalskii[J].Acta Botanica Boreal-Occidentalia Sinica, 2004, 24(7):1319.
[12]	鲁艳.哈密盆地裸果木群落物种多样性及其优势种群生态位研究[D]. 乌鲁木齐:新疆大学,2007:53. LU Yan.The study on psecies diversity and dominant populations niche of Gymnocarpos przewalskii Maxim community in Hami Basin[D]. Urumqi:Xinjiang University, 2007:53.
[13]	王立龙,王亮,张丽芳,等.不同生境下濒危植物裸果木种群结构及动态特征[J]. 植物生态学报, 2015, 39(10):980. WANG Lilong, WANG Liang, ZHANG Lifang, et al. Structure and dynamic characteristics of Gymoncarpos przewalskii in different habitats[J].Chinese Journal of Plant Ecology,2015, 39(10):980.
[14]	王博,王亮,王立龙,等.濒危植物裸果木(Gymnocarpos przewalskii)与其伴生种种间联结性及群落稳定性[J]. 中国沙漠, 2017, 37(1):86. WANG Bo,WANG Liang,WANG Lilong, et al. The interconnectedness and community stability of the Gymnocarpos przewalskii of endangered plants[J]. Journal of Desert Research, 2017, 37(1):86.
[15]	MA Songmei, ZHANG Mingli. Phylogeography and conservation genetics of the relic Gymnocarpos przewalskii (Caryophyllaceae) restricted to northwestern China[J]. Conserv Genet, 2012(13):1531.
[16]	陈国雄,李定淑,张志谦,等. 盐胁迫对西葫芦和黄瓜种子萌发影响的对比研究[J].中国沙漠, 1996, 16(3):307. CHEN Guoxiong, LI Dingshu, ZHANG Zhiqian, et al. Comparative studies of effect of salt stress on pumpkin and cucumber seed germination.[J]. Journal of Desert Research, 1996,16(3):307.
[17]	苏丹,李红丽,董智,等.盐胁迫对白榆无性系抗氧化酶活性及丙二醛的影响[J].中国水土保持科学,2016, 14(2),10. SU Dan,LI Hongli,DONG Zhi, et al. Effects of salt stress on activities of antioxidant enzymes and MDA of elm clones[J].Science of Soil and Water Conservation,2016,14(2),10.
[18]	卢艳敏.不同盐胁迫对高羊茅种子萌发的影响[J].草业科学,2012,29(7):1088. LU Yanmin.The influence of different salt stress on Festuca arundinaceaseed germination[J].Pratacultural Science,2012,29(7):1088.
[19]	张利霞,常青山,侯小改,等.不同钠盐胁迫对夏枯草种子萌发特性的影响[J].草业学报,2015, 24(3):177. ZHANG Lixia, CHANG Qingshan, HOU Xiaogai, et al. Effects of sodium salt stress on seed germination of Prunella vulgaris[J].Acta Prataulture Sinica, 2015, 24(3):177.
[20]	刘克彪,姜生秀.干旱和钠盐胁迫对罗布麻种子萌发的影响[J].草业学报,2016,25(5):214. LIU Kebiao, JIANG Shengxiu. Responses of Apoceynum venetum seed germination to drought and salt stress[J].Acta Prataulture Sinica, 2016, 25(5):214.
[21]	王金平,王舒甜,岳健敏,等.香樟幼苗对NaCl胁迫的生理响应[J].中国水土保持科学,2016,14(5),82. WANG Jinping,WANG Shutian,YUE Jianmin,et al. Physiological response of Cinnamomum camphora seedlings to NaCl stress[J].Science of Soil and Water Conservation, 2016,14(5),82.
[22]	杨景宁,王彦荣. PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J]. 草业学报, 2012, 21(6):23. YANG Jingning, WANG Yanrong. Effects of drought stress by PEG effects on seed germination of four desert plant species[J].Acta Prataulturae Sinica,2012, 21(6):23.
[23]	MUNS R, TESTER M. Mechanisms of salinity tolerance[J]. Annual Review of plant Biology, 2008(59):651.
[24]	何佳亮, 董开茂,郑健,等. NaCl和PEG胁迫对金露梅种子萌发及幼苗的影响[J]. 西北林学院学报, 2014, 29(4):123. HE Jialiang, DONG Kaimao, ZHENG Jian, et al. Effects of NaCl and PEG stress on seed germination and seedling growth of Potentilla fruticosa[J].Journal of Northwest Forestry University.2014, 29(4):123.
[25]	李志萍,张文辉,崔豫川. PEG模拟干旱胁迫对栓皮栎种子萌发及生长生理的影响[J]. 西北植物学报, 2013, 33(10):2043. LI Zhiping, ZHANG Wenhui, CUI Yuchuan. Effect of PEG simulated drought sterss on seed germination and growth physiology of Quercus variabilis[J].Acta Botanica Boreali-Occidentalia Sinica,2013, 33(10):2043.
[26]	陈小倩,徐庆国.PEG-6000干旱胁迫对5种牧草种子萌发的影响研究[J].中国农学通报.2015,31(26):7. CHEN Xiaoqian, XU Qingguo. Effects of PEG-6000 drought stress on seed germination of five forage grass species[J].Chinese Agricultural Science Bulletin,2015,31(26):7.
[27]	徐振明,宛涛, 蔡萍,等.PEG模拟干旱胁迫对罗布麻种子萌发及生理特性的影响[J].中国草地学报, 2015, 37(5):75. XU Zhengming, WAN Tao, CAI Ping, et al. Effects of PEG simulated drought stress on germination and physiological properties of Apocynum venetum seeds[J].Chinese Journal of Glassland,2015, 37(5):75.
[28]	周玲,王乃江, 张丽楠. PEG胁迫对文冠果种子萌发和幼苗生理特性的影响[J].西北植物学报, 2012, 32(11):2293. ZHOU Lin,WANG Naijiang,ZHANG Linan.Effect of PEG treatment on seed germination and growth of seedlings of Xanthoceras sorbifolia[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(11):2293.
[29]	汪建军,麻安卫,汪治刚,等.不同温度和PEG处理对中华羊茅种子萌发的影响[J].草业学报, 2016, 25(4):73. WANG Jianjun, MA Anwei, WANG Zhigang, et al. Effect of different temperature and moisture on seed germination of Festuca sinensis[J]. Acta Prataulture Sinica, 2016, 25(4):73.
[30]	蒋高明,常杰,高玉葆等.植物生理生态学[M].北京:高等教育出版社.2004,12:85. JIANG Gaoming,CHANG Jie,GAO Yubao,et al. Plant physiological ecology[M]. Beijing:Higher Education Press.2004,12:85.