Abstract：[Background] The ecological environment management in most closed small coal mines in central and western Guizhou had achieved obvious results. The natural growth of Pinus massoniana, Betula luminifera, and Neyraudia, plant communities had appeared in coal gangue dumps piled in the open air for many years. Discussing the change and correlation of the nutrient and microbial community diversity of coal gangue matrix under the different types of natural vegetation are of great significance.[Methods] A sample plot (20 m×10 m) was set up in the coal gangue yard, we investigated 3 types of vegetation (Pinus massoniana young forest,Betula luminifera+Castanea seguinii young forest, and Neyraudia grass) and collected their dominant plants recovered from coal gangue dumps in the closed coal mine area in central Guizhou, and collected the coal gangue matrix samples from 3 types of vegetation or the rhizosphere of dominant plants at the same time. Moreover, the available nutrient content of coal gangue matrix was determined by conventional analytical methods and the functional diversity analysis of microbial communities was performed using the ECO MicroPlate of BIOLOGTM.[Results] 1) In the process of natural vegetation restoration, the organic carbon(C) and the effective nitrogen (N) contents of the coal gangue matrix increased significantly, and the effective phosphorus (P) and potassium (K) contents also increased to varying degrees, and the order of their effects was B. luminifera+C. sequinii young forest >P. massoniana young forest >Neyraudia grass and there were significant differences in available N contents of coal gangue matrix among the three vegetation types, and the organic C and available N contents of coal gangue matrix under B. luminifera+ C. sequinii young forest or P. massoniana young forest was significantly higher than those of Neyraudia grass. Also, the order of contents of organic C and available N, P and K in the coal gangue matrix of the dominant plant root zone was B. luminifera>Neyraudia>P. massoniana. 2) After the natural restoration of vegetation, the microbial community richness index H and dominance index D of the coal gangue matrix were significantly higher than those of the bare coal gangue matrix, especially B. luminifera+ C. sequinii young forest. The microbial community H of coal gangue matrix under different vegetation types had a significantly positive correlation with the contents of organic matter C, available N, effective P and effective K. However, the microbial community D and available N contents were extremely significantly positively correlated.[Conclusions] In the process of natural vegetation restoration, the organic C and available N, P and K mass fractions and microbial community diversity of coal gangue matrix increased significantly, especially in the young forest of B. luminifera+ C. sequinii.
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LIU Yuansheng, CHEN Zuyong, LIU Fang, ZHU Jian, BU Tongda. Effects of naturally-restored vegetation on the available nutrients and microbial community diversity of coal gangue matrix. SSWC, 2023, 21(1): 47-54.
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2023, Vol. 21 
