Effects of soil phenol stress on the photosynthetic efficiency parameters of poplar
DOU Zhiyang1, REN Gai2, MIAO Dezhi3, DENG Haiyu3, SUN Yuxuan1, XU Han1, LI Hui4, ZHANG Guangcan1
1. Shandong Agricultural University, Shandong Taishan National Forest Ecosystem National Positioning Research Station, 271000, Tai'an, Shandong, China; 2. South China Institute of Environmental Sciences, Ministry of Ecology and Environment, 510655, Guangzhou, China; 3. Linyi Yishui County Water Conservancy Engineering Support Center, 276000, Linyi, Shandong, China; 4. Linyi University 276000, Linyi, Shandong, China
Abstract：[Background] Studies on phenol stress in poplar have mainly focused on the cumulative effects of soil phenol and inter-root effects, but there is a lack of studies on the effects of phenol in soil on photosynthesis in poplar. The present study aims to reveal the characteristics and mechanisms of soil phenol stress on photosynthetic efficiency of poplar, and to provide a plant physiological basis for a deeper understanding of the inhibitory effect of soil phenol accumulation on the growth of poplar.[Methods] In order to explore the characteristics and physiological mechanism of soil phenol stress on photosynthetic efficiency of poplar, one-year-old potted poplar (Populus×euramericana ‘Neva’) seedlings were used as experimental materials, and soil phenol mass concentration (T) of the second generation poplar stand was used as the reference value, (0.5T, 1.0T,1.5T, 2.0T, 2.5T, and 3.0T) and 0T (control) of phenol mass concentration treating soil were set, and the photosynthesis measuring instrument (CIRAS-2) was used to determine the photosynthesis response data of different leaves to 14 photosynthetic active radiations, and the chlorophyll fluorescence parameters were measured using the portable modulation fluorometer (FMS-2).[Results] 1) At phenol mass concentration <2.0T, the net photosynthetic rate (Pn) under the condition of photosynthetically active radiation (PAR) <400 μmol/(mol2·s) increased rapidly with PAR, and Pn under the condition of PAR >1 200 μmol/(mol2·s) increased slowly with PAR. The Pn under PAR <400 μmol/(mol2·s) condition decreased significantly when the phenol mass concentration was >2.0T, and the Pn showed a significant decreasing trend with increasing PAR under high light intensity. 2) At phenol mass concentrations between 2.5T and 3.0T, the apparent quantum yield (Φ) decreased by 52%-62% compared with the control, the light-saturated net photosynthetic rate (Pnmax) decreased by 45%-60% compared with the control, the light compensation point (LCP) increased by 12%-13% compared with the control, and the light saturation point (LSP) decreased by 40%-50%. 3) The maximal quantum yield of photosystem Ⅱ complex (Fv/Fm) with the increase in soil phenol mass concentration and continue to decrease, after the phenol mass concentration >1.5T, the decreasing degree in Fv/Fm increased, the effective quantum yield of PS Ⅱ photochemistry ΦPS Ⅱ continuously declined with the increase in soil phenol mass concentration, when the soil phenol mass concentration >1.0T, ΦPS Ⅱ declining degree increased significantly, and ΦPSⅡ declining degree was > the decline in Fv/Fm. The minimal fluorescence (F0), the maximal fluorescence (Fm) and the non-photochemical quenching (qN) gradually increased with the increase of soil phenol mass concentration.[Conclusions] Soil phenol stress had a significant inhibitory effect on photosynthetic efficiency of poplar, and photosynthesis was significantly inhibited under high phenol mass concentration. When the phenol mass concentration increased, the photosynthetic apparatus of poplar could be protected by increasing heat dissipation, which was a physiological countermeasure for poplar photosynthesis to tolerate phenol stress in soil.
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2022, Vol. 20 
