The microorganisms of archives, mainly molds, not only seriously affect the life and value of the archives, but also endanger the health of archivists, so the disinfection of archives is an important means to strengthen the protection of physical archives and occupational health protection.This paper compares and analyzes the disinfection efficiency of plasma ozone sterilization chamber and ozone fumigation sterilization chamber on paper archives through experimental methods. The experiment focuses on evaluating the paper penetration ability, effective dosage for disinfection, and disinfection effect. Various paper materials and molds were tested, and the results indicate that the plasma ozone sterilization chamber outperforms the traditional ozone fumigation sterilization chamber in the above aspects, making it more suitable for batch disinfection and sterilization tasks of paper archives. The findings provide a scientific basis for archives to choose disinfection technologies and equipment and are significant for the development of disinfection equipment and the advancement of archival protection techniques.