坡耕地土壤转化酶活性与芸豆产量对等高反坡阶措施的响应

doi:10.16843/j.sswc.2023114

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摘要为探究坡面微地形改造水土保持措施—等高反坡阶(CRT)对坡耕地土壤肥力的提升效果及其生态修复功能,有必要关注该措施对土壤养分与转化酶活的影响,及其提升作物产量的机制。通过分析坡耕地CRT处理后阶上和阶下0~5 cm土层养分(全氮TN、全磷TP、铵态氮NH₄⁺-N、硝态氮NO₃^--N和速效磷AP)及酶活(脲酶S-UE、β-1,4-N-乙酰葡萄糖苷酶S-NAG、亮氨酸氨基肽酶S-LAP和酸性磷酸酶S-ACP)差异,并以原状坡耕地(CK)相应位置作对照,探讨CRT措施下土壤养分转化相关酶对芸豆(Phaseolus vulgaris)产量和千粒质量的影响机制。结果表明:1) CRT使土壤TN、NO₃^--N、TP、AP含量较CK阶上分别提高21.2%、86.2%、14.1%和28.4%,阶下提高7.3%、77.4%、21.8%和30.1%;使NH₄⁺-N阶下提高2.8%,阶上降低22.5%。CRT对NO₃^--N的提升效果高于TN和NH₄⁺-N,对AP的提升效果优于TP。2) CRT措施使坡耕地S-UE、S-NAG、S-LAP和S-ACP活性阶上较CK分别提高13.4%、55.9%、56.8%和44.6%,阶下提高48.3%、72.3%、70.9%和34.6%,且阶下增幅大于阶上。3) CK坡耕地阶上、阶下芸豆产量为20.0和23.2 kg/hm²,千粒质量为448和376 g,而CRT使芸豆产量和千粒质量阶上提高66.7%和8.6%,阶下提高289%和26.6%,阶下增幅均大于阶上。4)相比CK,CRT使坡耕地芸豆产量与S-UE由不相关变为显著正相关(P<0.05),相关系数由0.12增至0.91,使坡耕地芸豆千粒质量与氮、磷、酶活(S-NAG、S-LAP、S-ACP)由显著负相关(P<0.05)变为相关。S-LAP、S-NAG、NH₄⁺-N对芸豆产量和千粒质量的影响显著(P<0.01),解释量分别为48.4%、29.2%、18.0%。CRT通过改善坡耕地土壤养分的空间分布,提高相关酶活,加速有机养分向无机养分的转化,提升土壤关键性肥力,进而促进芸豆生长发育,提高产量和千粒质量。

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	徐其静
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	李航
	汪丽
	王克勤

关键词 ：酶活性, 芸豆产量, 等高反坡阶, 土壤养分, 坡耕地

Abstract：[Background] In order to explore the improvement effect of slope microtopographic modification soil and water conservation measure-contour reverse-slope terrace (CRT) on soil fertility and its ecological restoration function in sloping farmland, it is necessary to pay attention to the effect of CRT on soil nutrients and enzyme activities related to nutrient conversion, as well as its contribution and mechanism to crop yield. [Methods] Soil nutrients (TN, TP, NH⁺₄-N, NO^-₃-N and AP) and soil urease (S-UE), soil β-1, 4-N-acetylglucosidase (S-NAG), soil leucine aminopeptidase (S-LAP) and acid phosphatase (S-ACP) were analyzed in the sloping farmland 0-5 cm soil of two terrace positions (aboveandbelow the terrace) under CRT treatments, and the corresponding position of the unaltered sloping farmland (CK) was taken as the control. The effects and mechanism of soil nutrient conversion related enzymes on yield and 1 000-grain weight (TGW) of kidney bean (Phaseolus vulgaris) under CRT were investigated. [Results] 1) Compared to CK, the contents of soil TN, NO^-₃-N, TP and AP in CRT increased by 21.2%, 86.2%, 14.1% and 28.4% in the slope above the terrace, but 7.3%, 77.4%, 21.8% and 30.1% in the slope below the terrace. The NH⁺₄-N increased by 2.8% in the slope above the terrace and decreased by 22.5% below the terrace in CRT. The enhancement effect on NO^-₃-N was higher than that on TN and NH⁺₄-N, and the enhancement effect on AP was better than that on TP. 2) Compared to CK, the S-UE, S-NAG, S-LAP and S-ACP activities increased by 13.4%, 55.9%, 56.8% and 44.6% in above the terrace of CRT, and 48.3%, 72.3%, 70.9% and 34.6% in below the terrace of CRT. Moreover, the increase rate of below the terrace was greater than that of above the terrace. 3) The per hectare yield of kidney bean were 20.0 and 23.2 kg/hm², and TGW were 448 and 376 g in CK sloping farmland (above and below the terrace), while yield increased by 66.7% and 289% (above and below the terrace), TGW increased by 8.6% and 26.6% (above and below the terrace) under CRT treatment. And the increase rate of below the terrace was greater than that of above the terrace. 4) Compared with CK, CRT changed kidney bean yield and S-UE from non-correlation to significant positive correlation (P<0.05), the correlation coefficient (R) increased from 0.12 to 0.91, and the TGW of kidney beans in slopingfarmland land changed from negative correlation (P<0.05) to correlation with nitrogen, phosphorus nutrients and enzymes (S-NAG, S-LAP and S-ACP). S-LAP, S-NAG and NH⁺₄-N had significant effects on yield and TGW of kidney bean (P<0.01), explains rate were 48.4%, 29.2% and 18.0%, respectively. [Conclusions] CRT accelerates the conversion of organic nutrient to inorganic nutrient, promotes the growth and development of kidney bean, and increases the yield and TGW by improving the spatial distribution of soil nutrients, which increases the activities of related enzymes in sloping farmland.

Key words： enzyme activities kidney bean yield contour reverse-slope terrace soil nutrient sloping farmland

收稿日期: 2023-07-27

PACS:

S158.3

基金资助:国家重点研发计划重点专项课题“西南高山峡谷民族聚集区水土保持生态产业发展技术与模式”(2022YFF1302904);云南省教育厅科学研究基金项目“等高反坡阶对坡耕地退化土壤的生态修复机理研究”(2023Y0710),“田间水保工程对红壤坡耕地团聚体微生物量时空分布的影响研究”(2023Y0712);云南省科技厅农业联合面上项目“野生鸡枞菌对土壤重金属的生物累积机制及基于生物有效性的风险评价模型研究”(202301BD070001-031);云南省科技厅青年基金项目“基于生态化学计量学的抚仙湖流域坡耕地面源污染控制机制研究”(202101AU070008)

通讯作者: 王克勤(1964—),男,博士,教授。主要研究方向:山区小流域环境综合治理。E-mail:wangkeqin7389@sina.com E-mail: wangkeqin7389@sina.com

作者简介: 徐其静(1992—),女,博士研究生,助理实验师。主要研究方向:坡耕地土壤退化与生态修复。E-mail:xuqijingjing@126.com

引用本文:

徐其静, 侯磊, 李加豪, 李奇奇, 李航, 汪丽, 王克勤. 坡耕地土壤转化酶活性与芸豆产量对等高反坡阶措施的响应[J]. 中国水土保持科学, 2024, 22(5): 105-114.
XU Qijing, HOU Lei, LI Jiahao, LI Qiqi, LI Hang, WANG Li, WANG Keqin. Responses of soil conversion enzyme activities and kidney bean yield to contour reverse-slope terrace treatment in sloping farmland. SSWC, 2024, 22(5): 105-114.

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http://journal12.magtechjournal.com/Jweb_stbc/CN/10.16843/j.sswc.2023114 或 http://journal12.magtechjournal.com/Jweb_stbc/CN/Y2024/V22/I5/105

[1]	DENG Lei, PENG Changhui, HUANG Chunbo, et al. Drivers of soil microbial metabolic limitation changes along a vegetation restoration gradient on the Loess Plateau, China [J]. Geoderma, 2019, 353: 188.
[2]	HOANG D T T, RAZAVI B S, KUZYAKOV Y, et al. Earthworm burrows: kinetics and spatial distribution of enzymes of C-, N- and P-cycles [J]. Soil Biology and Biochemistry, 2016, 99: 94.
[3]	曹越,赵洋毅,王克勤, 等.滇中坡改梯不同种植方式对土壤酶活性的影响[J]. 西部林业科学,2020,49(4):91. CAO Yue, ZHAO Yangyi, WANG Keqin, et al. Effects of different planting methods on soil enzyme activity in central Yunnan province [J]. Journal of West China Forestry Science, 2020,49(4):91.
[4]	肖海兵, 李忠武, 聂小东, 等. 南方红壤丘陵区土壤侵蚀—沉积作用对土壤酶活性的影响[J]. 土壤学报, 2016, 53(4): 881. XIAO Haibing, LI Zhongwu, NIE Xiaodong, et al. Effects of soil erosion and deposition on soil enzyme activity in hilly red soil regions of South China [J]. Acta Pedologica Sinica, 2016, 53(4): 881.
[5]	陈正发,李靖,段青松,等.基于USLE模型的云南省坡耕地土壤侵蚀和养分流失评价[J].农业工程学报,2022,38(16):124. CHEN Zhengfa, LI Jing, DUAN Qingsong, et al. Evaluation of soil erosion and nutrient loss of slope farmland in Yunnan province using USLE model [J]. Transactions of the CSAE, 2022, 38(16): 124.
[6]	耿华杰,郑粉莉,莫帅豪,等.典型黑土区农业流域土壤侵蚀—沉积对土壤养分和酶活性的影响[J]. 水土保持学报, 2023, 37(4): 47. GENG Huajie, ZHENG Fenli, MO Shuaihao, et al. Effects of soil erosion-deposition on soil nutrients and enzyme activities in agricultural watershed of Chinese mollisol region [J]. Journal of Soil and Water Conservation, 2023,37(4): 47.
[7]	肖理,王章文,殷庆元,等.金沙江干热河谷坡改梯对水土保持的影响[J].西南农业学报,2019,32(12):2856. XIAO Li, WANG Zhangwen, YIN Qingyuan, et al. Effect of terracing slope cropland on soil and water conservation in dry-hot valley of Jinsha river basin, southwest China [J]. Southwest China Journal of Agricultural Sciences, 2019, 32(12): 2856.
[8]	陈雪, 宋娅丽, 王克勤, 等. 基于Van Genuchten模型的等高反坡阶下土壤水分特征[J]. 水土保持研究, 2019, 26(5): 45. CHEN Xue, SONG Yali, WANG Keqin, et al. Moisture characteristics under contour reverse-slope terrace based on Van Genuchten model[J]. Research of Water and Soil Conservation, 2019, 26(5): 45.
[9]	刘晓微, 王克勤, 赵洋毅, 等. 等高反坡阶措施下玉米苗期根系分泌物特征及其对根际土壤酶活性的响应[J]. 四川农业大学学报, 2021, 39(4): 477. LIU Xiaowei, WANG Keqin, ZHAO Yangyi, et al. Characteristics of maize root exudates at seedling stage and their response to rhizosphere soil enzyme activities under contour reverse-slope terrace [J]. Journal of Sichuan Agricultural University, 2021, 39(4): 477.
[10]	张洋, 王克勤, 段旭, 等. 等高反坡阶措施下玉米水分利用效率对坡耕地土壤水分变化的响应[J]. 西北农林科技大学学报(自然科学版), 2022, 50(1): 112. ZHANG Yang, WANG Keqin, DUAN Xu, et al. Response of crop water use efficiency to soil water changes on contour-reverse slope [J]. Journal of Northwest A & F University (Natural Science Edition), 2022, 50(1): 112.
[11]	王珮环,张晴雯,石玉龙,等.秸秆覆盖和配施有机肥对侵蚀坡耕地土壤胞外酶化学计量特征的影响[J]. 植物营养与肥料学报,2023,29(3):459. WANG Peihuan, ZHANG Qingwen, SHI Yulong, et al.Effects of straw mulching and organic fertilizer on the stoichiometry of soil extracellular enzymes in eroded slope farmland [J]. Journal of Plant Nutrition and Fertilizers, 2023,29(3):459.
[12]	陈敏全,王克勤.等高反坡阶对坡耕地土壤碳库的影响[J].水土保持通报,2015,35(6):41. CHEN Minquan, WANG Keqin. Effects of reverse-slope level terrace on soil carbon stock of sloping farmland [J]. Bulletin of Soil and Water Conservation, 2015,35(6):41.
[13]	王帅兵, 宋娅丽, 王克勤, 等. 不同雨型下等高反坡阶对红壤坡耕地氮、磷流失的影响[J]. 农业工程学报, 2018, 34(13): 160. WANG Shuaibing, SONG Yali, WANG Keqin, et al. Effects of reverse-slope terrace on nitrogen and phosphorus loss in sloping farmland of red loam under different rainfall patterns [J]. Transactions of the CSAE, 2018, 34(13): 160.
[14]	王帅兵,王克勤,宋娅丽,等.等高反坡阶对昆明市松华坝水源区坡耕地氮、磷流失的影响[J].水土保持学报,2017,31(6):39. WANG Shuaibing, WANG Keqin, SONG Yali, et al. Effects of contour reverse-slope terrace on nitrogen and phosphorus loss in sloping farmland in the water resource area of Songhua Dam in Kunming city [J]. Journal of Soil and Water Conservation, 2017,31(6):39.
[15]	曹鸿景, 王克勤, 冯晓月. 松华坝水源区等高反坡阶对坡耕地土壤氮含量的影响[J]. 西南林业大学学报(自然科学版), 2017, 37(5): 130. CAO Hongjing,WANG Keqin,FENG Xiaoyue. Effect of reverse-slope level terrace on the nitrogen contentsof sloping farmland in Songhuaba reservoir [J]. Journal of Southwest Forestry University (Natural Science Edition), 2017, 37(5): 130.
[16]	华锦欣,王克勤,张香群, 等.昆明松华坝水源区等高反坡阶对坡耕地土壤磷含量的影响研究[J]. 中南林业科技大学学报,2016,36(3):76. HUA Jinxin, WANG Keqin, ZHANG Xiangqun, et al.Effect of reverse-slope level terrace on the phosphorus contents of sloping farmland in Kunming Songhuaba reservoir [J]. Journal of Central South University of Forestry & Technology, 2016,36(3):76.
[17]	黄艳荟,宁嘉丽,李桂芳,等.甘蔗种植方式对蔗地土壤侵蚀及氮素流失特征的影响[J]. 水土保持通报,2022,42(6):121. HUANG Yanhui, NING Jiali, LI Guifang, et al. Effects of planting mode on soil erosion and nitrogen loss in a sugarcane field [J]. Bulletin of soil and Water Conservation, 2022,42(6):121.
[18]	王双,叶良惠,郑子成,等.玉米成熟期黄壤坡耕地径流及其氮素流失特征研究[J]. 水土保持学报,2018,32(6): 28. WANG Shuang, YE Lianghui, ZHENG Zicheng, et al. Characteristics of runoff and nitrogen loss in yellow soil sloping cropland at mature stage of maize [J]. Journal of Soil and Water Conservation, 2018,32(6): 28.
[19]	王荣嘉,张建锋,蔡春菊,等.不同土地利用方式对长三角水源地氮磷流失的影响[J]. 水土保持研究,2023,30(1):128. WANG Rongjia, ZHANG Jianfeng, CAI Chunju, et al. Effects of different land use on nitrogen & phosphorus loss of water source area in Yangtze River Delta [J]. Research of Water and Soil Conservation, 2023,30(1):128.
[20]	吴金芝,黄修利,侯园泉,等.垄沟种植对旱地玉—麦轮作体系生产力和土壤硝态氮累积量的影响[J].中国农业科学,2023,56(11):2078. WU Jinzhi, HUANG Xiuli, HOU Yuanquan, et al.Effects of ridge and furrow planting patterns on crop productivity and soil nitrate-N accumulation in dryland summer maize and winter wheat rotation system [J]. Scientia Agricultura Sinica, 2023,56(11):2078.
[21]	唐佐芯, 王克勤, 李秋芳, 等. 等高反坡阶对坡耕地产流产沙和氮磷迁移的作用研究[J]. 水土保持研究, 2013, 20(1): 1. TANG Zuoxin, WANG Keqin, LI Qiufang, et al. Study on contour reverse-slope terrace controlling soil and water loss and nitrogen and phosphorus transfer in the sloping farmland [J]. Research of Water and Soil Conservation, 2013, 20(1): 1.
[22]	李星辰, 杨吉华, 于连家,等.石灰岩山地不同整地方式对侧柏林土壤蓄水保土功能的影响[J]. 中国水土保持科学,2013, 3(11): 59. LI Xingchen, YANG Jihua, YU Lianjia, et al. Influence of different site preparation modes on the function of soil and water conservation of Platycladus orientalis forest in Limestone Mountainous region [J]. Science of Soil and Water Conservation, 2013, 3(11): 59.
[23]	张瑜,徐子棋,杨献坤, 等.东北黑土区山地丘陵区坡面水土保持措施效益研究[J]. 中国水土保持,2022(12):36. ZHANG Yu, XU Ziqi, YANG Xiankun, et al. Benefits of slope soil and water conservation measures in northeast black soil region [J]. Soil and Water Conservation in China, 2022(12):36.
[24]	ZHENG Huabin, TANG Qiyuan, FU Zhiqiang,et al.Effects of micro-topographic reestablishment on paddy impoundment and rice yield [J]. Agricultural Science & Technology,2018,19(2):49.
[25]	李学峰, 王克勤, 宋娅丽, 等. 等高反坡阶对滇中云南松林生态系统碳储量及增量分配格局的影响[J]. 水土保持研究, 2019, 26(5): 21. LI Xuefeng, WANG Keqin, SONG Yali, et al. Effects of contour reverse-slope terrace on carbon storage and incremental distribution Pinus yunanensis forest ecosystem in middle of Yunnan province [J]. Research of Water and Soil Conservation, 2019, 26(5): 21.