Effects of Bacillus subtilis on the seed germination and seeding growth and physiological characteristics of psammophytes
WU Weiting1,2, LIU Zhenting1,2, GAO Guanglei1,2,3, SUN Zhe4, ZHANG Jinxin5, WANG Tianbo5, CAO Hongyu1,2, DING Guodong1,2
1. School of Soil and Water Conservation, Beijing Forestry University, 100083, Beijing, China; 2. Yanchi Ecology Research Station of the Mu Us Desert, 751500, Yanchi, Ningxia, China; 3. State Key Laboratory of Efficient Production of Forest Resources, 100083, Beijing, China; 4. China Electric Power Planning & Engineering Institute, 100120, Beijing, China; 5. China Power Engineering Consulting Group Co. Ltd., 100120, Beijing, China
Abstract：[Background]Bacillus subtilis is an emerging microbial material which potentially contribute to desertification combating. However, as a typical plant growth-promoting rhizobacteria, its effects on the plant colonization and growth in a desert environment still remain unclear. This study is aimed to investigate the effect of B.subtilis on the physiological characteristics of seed germination and seeding growth of psammophyte. [Methods] The controlled experiments were conducted to determine the seed germination, seeding growth and physiological characteristics of Ammopiptanthus mongolicus and Caragana korshinskii using different bacterial application amount (0, 1, 3, 5, 7 and 10 g/kg). The changes of seed germination and the physiological characteristics of seeding growth were investigated, including contents of chlorophyll, malondialdehyde and proline. [Results] 1) B. subtilis increased the seed germination rate of A. mongolicus and C. korshinskii, and the optimal dosage were 3 and 10 g/kg, respectively. Meanwhile, it had no significant effect on the seedling crown (P>0.05), only 10 g/kg dosage significantly impacted the height of C. korshinskii seedings (P<0.05). 2) B. subtilis significantly affected the physiological and biochemical characteristics of A. mongolicus and C. korshinskii (P<0.05). The chlorophyll content of A. mongolicus significantly increased (P<0.05) and the malondialdehyde and proline of A. mongolicus and C. korshinskii significantly decreased (P<0.05), and the optimal dosage was 5 g/kg. [Conclusions]B. subtilis improves the plant physiological and biochemical characteristics therefore promotes the seeding growth, but this effect is limited with a lagging performance. Moreover, it varies with the bacterial application amount and plant species, the C. korshinskii is more impressionable than A. mongolicus. This improved information will provide theoretical basis and scientific support for desertification combating technology exploration using B. subtilis.
吴玮婷, 刘振婷, 高广磊, 孙哲, 张金鑫, 王天博, 曹红雨, 丁国栋. 枯草芽孢杆菌对沙生植物种子萌发和幼苗生长生理特征的影响[J]. 中国水土保持科学, 2024, 22(6): 70-76.
WU Weiting, LIU Zhenting, GAO Guanglei, SUN Zhe, ZHANG Jinxin, WANG Tianbo, CAO Hongyu, DING Guodong. Effects of Bacillus subtilis on the seed germination and seeding growth and physiological characteristics of psammophytes. SSWC, 2024, 22(6): 70-76.
居炎飞, 邱明喜, 朱纪康, 等. 我国固沙材料研究进展与应用前景[J]. 干旱区资源与环境, 2019, 33(10): 138. JU Yanfei, QIU Mingxi, ZHU Jikang, et al. Research progress and application prospect of sand-fixing materials in China[J]. Journal of Arid Land Resources and Environment, 2019, 33(10): 138.
[2]
杜宇佳, 高广磊, 陈丽华, 等. 土壤微生物膜对风沙土固沙保水特性的影响[J]. 农业工程学报, 2020, 36(17): 98. DU Yujia, GAO Guanglei, CHEN Lihua, et al. Effects of soil microbial membrane on sand fixation and water retention of aeolian soil[J]. Transactions of the CSAE, 2020, 36(17): 98.
[3]
HSU C M, HUANG Y H, NIMJE V R, et al. Comparative study on the sand bioconsolidation through calcium carbonate precipitation by Sporosarcina pasteurii and Bacillus subtilis[J]. Crystals, 2018, 8(5): 189.
[4]
刘帅, 赵西宁, 李钊, 等. 不同改良剂对旱地苹果园壤土团聚体和水分的影响[J]. 水土保持学报, 2021, 35(2): 193. LIU Shuai, ZHAO Xining, LI Zhao, et al. Effects of different modifiers on soil aggregate and water content in dryland apple orchards[J]. Journal of Soil and Water Conservation, 2021, 35(2): 193.
[5]
PARK S A, BHATIA S K, PARK H A, et al. Bacillus subtilis: As a robust host for biochemical production utilizing biomass[J]. Critical Reviews in Biotechnology, 2021, 41(6): 827.
[6]
XU Weifang, REN Huishuang, OU Ting, et al. Genomic and functional characterization of the endophytic Bacillus subtilis 7PJ-16 strain, a potential biocontrol agent of mulberry fruit sclerotiniose[J]. Microbial Ecology, 2018, 77(3): 651.
[7]
ONGENA M, JACQUES P. Bacillus lipopeptides: Versatile weapons for plant disease biocontrol[J]. Trends in Microbiology, 2007, 16(3): 115.
[8]
ZHANG Huihui, LIU Yunpeng, WU Gengwei, et al. Bacillus velezensis tolerance to the induced oxidative stress in root colonization contributed by the two-component regulatory system sensor ResE[J]. Plant Cell and Environment, 2021, 44(9): 3094.
[9]
PENHAR O, VANDENBERGHE L P S, FAULDS C, et al. Bacillus lipopeptides as powerful pest control agents for a more sustainable and healthy agriculture: Recent studies and innovations[J]. Planta, 2020, 251(3): 70.
[10]
曹成有, 蒋德明, 阿拉木萨, 等. 小叶锦鸡儿人工固沙区植被恢复生态过程的研究[J]. 应用生态学报, 2000, 11(3): 349. CAO Chengyou, JIANG Deming, ALA Musa, et al. Ecological process of vegetation restoration in artificial sand fixation area of Caragana korshinskii[J]. Chinese Journal of Applied Ecology, 2000, 11(3): 349.
[11]
刘果厚. 阿拉善荒漠特有植物沙冬青濒危原因的研究[J]. 植物研究, 1998, 18(3): 341. LIU Guohou. Study on the endangered causes of Ilex mongolica endemic to Alxa desert[J]. Plant research, 1998, 18(3): 341.
[12]
李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2003: 134. LI Hesheng. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing: Higher Education Press, 2003: 134.
[13]
NEVHULAUDZI T, NTUSHELO K, KANU S A, et al. Effect of Bacillus subtilis and elevated temperature on seed germination indices of three cowpea genotypes[J]. South African Journal of Botany, 2018, 115: 302.
[14]
VANSTRAELEN M, BENKOVA E. Hormonal interactions in the regulation of plant development[J]. Annual Review of Cell and Developmental Biology, 2012, 28: 463.
[15]
刘鲁峰, 狄义宁, 何丽莲, 等. 内生枯草芽孢杆菌B9促生长效果及产生吲哚乙酸(IAA)能力研究[J]. 云南农业大学学报(自然科学), 2020, 35(2): 227. LIU Lufeng, DI Yining, HE Lilian, et al. Study on growth promoting effect of endophytic Bacillus subtilis B9 and its ability to produce indoleacetic acid (IAA)[J]. Journal of Yunnan Agricultural University (Natural Sciences), 2020, 35(2): 227.
[16]
JANG J H, KIM S H, KHZINE I, et al. Physiological changes and growth promotion induced in poplar seedlings by the plant growth-promoting rhizobacteria Bacillus subtilis JS[J]. Photosynthetica, 2018, 56(4): 1188.
JAVARAJ J, YI H, LIANG G H, et al. Foliar application of Bacillus subtilis AUBS1 reduces sheath blight and triggers defense mechanisms in rice[J]. Journal of Plant Disease and Protection, 2004, 111(2): 115.
[19]
裴斌, 张光灿, 张淑勇, 等. 土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J]. 生态学报, 2013, 33(5): 1386. PEI Bin, ZHANG Guangcan, ZHANG Shuyong, et al. Effects of soil drought stress on photosynthesis and antioxidant enzyme activities in seabuckthorn leaves[J]. Acta Ecologica Sinica, 2013, 33(5): 1386.
[20]
王竞红, 张秀梅, 陈艾, 等. 紫穗槐幼苗根系生理特性和解剖结构对PEG-6000模拟干旱的响应[J]. 生态学报, 2018, 38(2): 511. WANG Jinghong, ZHANG Xiumei, CHEN Ai, et al. Responses of root physiological characteristics and anatomical structure of Robinia fruticosa seedlings to PEG-6000 simulated drought[J]. Acta Ecologica Sinica, 2018, 38(2): 511.
[21]
CHOWDAPPA P, KUMAR S P M, LAKSHMI M J, et al. Growth stimulation and induction of systemic resistance in tomato against early and late blight by Bacillus subtilis OTPB1 or Trichoderma harzianum OTPB3[J]. Biological Control, 2013, 65(1): 109.
[22]
王翠平, 严莉, 乔改霞, 等. 脯氨酸通过活性氧信号抑制植物生长[J]. 植物生理学报, 2017, 53(9): 1788. WANG Cuiping, YAN Li, QIAO Gaixia, et al. Proline inhibits plant growth through reactive oxygen species signaling[J]. Plant Physiology Journal, 2017, 53(9): 1788.
2024, Vol. 22 
