Abstract：[Background] The utilization efficiency of nitrogen fertilizer indicates the ability of crops to absorb nitrogen from soil and fertilizer. At the same time, nitrogen absorption and accumulation are the basis of crop yield formation. It is helpful to find out the relationship between the increase of crop yield and the efficient use of nutrients by investigating the accumulation of nitrogen uptake by crops. With a view to investigating the destination of nitrogen fertilizer and its use efficiency in plants under different farming modes, this paper explores the nitrogen use efficiency of plans employing the 15N isotopic tracer technique.[Methods] Three mulching modes, namely, biochar regulation (M1), combined regulation of biochar and straw (M2) and straw regulation (M3) were set up in the course of experiment, each containing three gradients, namely, 6 t/hm2 (F1), 12 t/hm2 (F2) and 18 t/hm2 (F3). The adsorption, distribution and use effect of nitrogen fertilizer in summer maize under different mulching control measures were investigated by analyzing the content change of marked nitrogen fertilizer in soil, the accumulation of nitrogen in plants and the source of nitrogen in each organ according to the field experiment.[Results] 1) The heat-retaining and water-holding capacity of straw and the physical and chemical properties of biochar significantly increased the content of inorganic nitrogen in soil. At the shooting stage, the average content of inorganic nitrogen in 0-80 cm soil layer under the M2F1 treatment condition was 11.71 and 20.25 mg/kg and higher than that under the M1FI and M3F1 conditions respectively. At the maturation stage, this average value under the M2F1 treatment condition was also remarkably higher than that under the M1F1 and M3F1 conditions. 2) Soil mulching regulation was effective in enhancing the accumulation effect of nitrogen in plants. The increase of mulching was accompanied by the marked rise of nitrogen content coming from nitrogen fertilizer. As shown by the analysis of the adsorption of labeled nitrogen by each organ, the results of 15N use efficiency in organs were as follows:Grain > Stem +leaf sheath > Root > Blade > Loquat + cob. The combined regulation of straw and biochar guaranteed the strongest nitrogen adsorption effect. 3) The results from Pearson and related analysis demonstrated that the biochar regulation effectively promoted the adsorption of labeled nitrogen fertilizer by root system, the combined regulation of biochar and straw facilitated the regulating effect of grain on marked nitrogen fertilizer and straw boosted the accumulation of marked nitrogen fertilizer in stalk + sheath.[Conclusions] The research may provide theoretical reference and technical support for the reasonable and effective development of agricultural water and soil resources and scientific guidance on agricultural fertilizer usage.
王铭浩, 王秀茹, 孙妍. 生物质覆盖对玉米植株氮素吸收和分配的影响[J]. 中国水土保持科学, 2020, 18(2): 120-129.
WANG Minghao, WANG Xiuru, SUN Yan. Nitrogen absorption, distribution and utilization efficiency of summer maize plants under different biomass covering. SSWC, 2020, 18(2): 120-129.
SOSA L L D, GLANVILLE H C, MARSHALL M R, et al. Spatial zoning of microbial functions and plant-soil nitrogen dynamics across a riparian area in an extensively grazed livestock system[J]. Soil Biology & Biochemistry, 2018(120):153.
[2]
银敏华, 李援农, 李昊,等. 氮肥运筹对夏玉米根系生长与氮素利用的影响[J]. 农业机械学报, 2016, 47(6):129. YIN Minhua, LI Yuannong, LI Hao, et al. Effects of nitrogen application rates on root growth and nitrogen use of summer maize[J]. Transactions of the CSAM, 2016, 47(6):129.
[3]
虞凯浩, 陈效民, 陈旭,等. 模拟气候变化条件下太湖地区典型农田氮素的动态变化[J]. 水土保持学报, 2015,29(1):96. YU Kaihao, CHEN Xiaomin, CHEN Xu, et al. Soil nitrogen DNAMIC changes in typical farmland of Taihu region under simulating climate change[J]. Journal of Soil and Water Conservation, 2015,29(1):96.
[4]
李广浩, 董树亭, 赵斌,等. 不同土壤水分状况下实现夏玉米高产及氮素高效的控释尿素用量研究[J]. 植物营养与肥料学报, 2018,24(3):579. LI Guanghao, DONG Shuting, ZHAO Bin, et al. Optimal application rates of controlled release urea for high yield and high nitrogen use efficiency of summer maize under different soil water conditions[J]. Plant Nutrition and Fertilizer Science, 2018,24(3):579.
[5]
张洪培, 陈迎迎, 沈玉芳,等. 旱地农田春玉米氮素吸收利用对栽培模式的响应[J]. 干旱地区农业研究, 2017, 35(4):205. ZHANG Hongpei, CHEN Yingying, SHEN Yufang, et al. Response of nitrogen accumulation and utilization to the cultivation mode in dryland spring maize field[J]. Agricultural Research in the Arid Areas, 2017, 35(4):205.
[6]
袁晶晶, 同延安, 卢绍辉,等. 生物炭与氮肥配施改善土壤团聚体结构提高红枣产量[J]. 农业工程学报, 2018, 34(3):159. YUAN Jingjing, TONG Yan'an, LU Shaohui, et al. Biochar and nitrogen amendments improving soil aggregate structure and jujube yields[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2018, 34(3):159.
[7]
PANDIT N R, MULDER J, HALE S E, et al. Multi-year double cropping biochar field trials in Nepal:Finding the optimal biochar dose through agronomic trials and cost-benefit analysis.[J]. Science of the Total Environment, 2018, 637(1):1333.
[8]
LIU Z F, HAN Y T, JING M, et al. Sorption and transport of sulfonamides in soils amended with wheat straw-derived biochar:effects of water pH, coexistence copper ion, and dissolved organic matter[J]. Journal of Soils and Sediments, 2017, 17(3):771.
[9]
于庆峰, 苗庆丰, 史海滨, 等. 秸秆覆盖量对土壤温度和春玉米耗水规律及产量的影响[J]. 水土保持研究, 2018, 25(3):111. YU Qingfeng, MIAO Qingfeng, SHI Haibin, et al. Effects of straw mulch rates on soil temperature, water use and yield of spring maize[J]. Research of Soil and Water Conservation, 2018, 25(3):111.
[10]
RAM H, DADWHAL V, VASHIST K K, et al. Grain yield and water use efficiency of wheat (triticum aestivum L.) in relation to irrigation levels and rice straw mulching in North West India[J]. Agricultural Water Management, 2013(128):92.
[11]
高敬尧, 王宏燕, 许毛毛, 等. 生物炭施入对农田土壤及作物生长影响的研究进展[J]. 江苏农业科学, 2016, 44(10):10. GAO Jingyao, WANG Hongyan, XU Maomao, et al. Research progress on the effects of biochar application on farmland soil and crop growth[J]. Jiangsu Agricultural Sciences, 2016, 44(10):10.
[12]
BRUUN E W, PETERSEN C T, HANSEN E, et al. Biochar amendment to coarse sandy subsoil improves root growth and increases water retention[J]. Soil Use Manage, 2014(30):109.
[13]
邹娟, 胡学玉, 张阳阳, 等. 不同地表条件下生物炭对土壤氨挥发的影响[J]. 环境科学, 2018, 39(1):348. ZOU Juan, HU Xueyu, ZHANG Yangyang, et al. Effect of biochar on ammonia volatilization from soils of different surface conditions[J]. Environmental Science. 2018, 39(1):348.
[14]
UZOMA K C, INOUE M, ANDRY H, et al. Effect of cow manure biochar on maize productivity under sandy soil condition[J]. Soil Use and Management, 2011, 27(2):205.
[15]
何绪生, 张树清, 佘雕, 等. 生物炭对土壤肥料的作用及未来研究[J]. 中国农学通报, 2011, 27(15):16. HE Xusheng, ZHANG Shuqing, SHE Diao, et al. Effects of biochar on soil and fertilizer and future research[J]. Chinese Agricultural Science Bulletin, 2011, 27(15):16.
[16]
魏永霞, 冯鼎锐, 刘志凯,等. 生物炭对黑土区坡耕地水土保持及大豆增产效应研究[J]. 节水灌溉, 2017(5):37. WEI Yongxia, FENG Dingrui, LIU Zhikai, et al. The effect of biochar on soil and water conservation and crop yield of the slope farmland in black soil region[J]. Water Saving Irrigation, 2017(5):37.
[17]
LIANG B, LEHMANN J, SOLOMON D, et al. Black carbon increases cation exchange capacity in soils[J]. Soil Science Society of America Journal, 2006, 70(5):1719.
[18]
BAIAMONTE G, CRESCIMANNO G, PARRINO F, et al. Effect of biochar on the physical and structural properties of a sandy soil[J]. Catena, 2019, 175:294.
[19]
勾芒芒, 屈忠义. 土壤中施用生物炭对番茄根系特征及产量的影响[J]. 生态环境学报, 2013,22(8):1348. GOU Mangmang, QU Zhongyi. Effect of biochar on root distribution and yield of tomato in sandy loam soil[J]. Ecology and Environmental Sciences, 2013, 22(8):1348.
[20]
李瑞霞, 李洪杰, 霍艳丽,等. 生物炭对华北冬小麦根系形态和内生真菌多样性的影响[J]. 农业机械学报, 2018, 49(3):235. LI Ruixia, LI Hongjie, HUO Yanli, et al. Effect of biochar on root morphology and endophytic fungal diversity of winter wheat in North China[J]. Transactions of The Chinese Society of Agricultural Machinery, 2018, 49(3):235.
[21]
文廷刚, 王维新, 杜小凤,等. 砂姜黑土区秸秆还田与肥料配施对夏玉米生育和产量的影响[J]. 中国农学通报, 2015, 31(17):156. WEN Tinggang, WANG Weixin, DU Xiaofeng, et al. Effects of wheat straw returned to field and combined fertilizer application on growth and yield of summer maize in lime concretion black soil area[J]. Chinese Agricultural Science Bulletin, 2015, 31(17):156.
2020, Vol. 18 
