Abstract：[Background]Heritiera littoralis is an endangered semi mangrove tree species. Recently, the community structure, biological characteristics, medicinal value, fruit characteristics and germination technology and genetic diversity of H. littoralis have mainly been analyzed by scholars, but the high CO2 concentration on photosynthetic characteristics in the leaves of H. littoralis seedlings under salinity treatment has been less investigated. It is important to explore the effects of elevated CO2 on photosynthesis characteristics in H. littoralis under salinity treatment, which is essential to provide references for the application of H. littoralis to the vegetation restoration in coastal areas. [Methods] 1-year-old H. littoralis seedlings were used for comparison test in the green house of Guangdong Academy of Forestry in Guangzhou. Salinity treatment was simulated using salt solution concentration gradients of 0 (CK) and 2.5% (salinity treatment) at different CO2 concentration (400, 600 and 800 μmol/mol). There were 16 repeats in each concentration, thus totally 96 pots. After 60 days, photosynthetic physiological parameters were measured by LI-6800 photosynthesis instrument (LI-COR, USA). Then modified rectangular hyperbolic model was adopted to fit the photosynthesis light-response curves at different CO2 concentration and CO2 response curves of H. littoralis leaves of salinity treatment. [Results] 1) After the experiment treatment, with the increase of CO2 concentration, net photosynthetic rate (Pn), maximum net photosynthetic rate (Pnmax), apparent quantum yield (AQY), light saturation point (LSP), dark respiration rate (Rd), water use efficiency (WUE) and intercellular CO2 concentration (Ci) in the leaves of H. littoralis seedlings increased, while light compensation point (LCP), transpiration rate (Tr) and stomatal conductance (Gs) decreased. 2) At the same CO2 concentration, Pn, Pnmax, AQY, LSP, LCP, Rd, Tr and Gs under salinity treatment were lower than those under control, while the difference of WUE and Ci between the two treatment groups were not significant. 3) With the increase of photosynthetic active radiation (PAR), Tr and Gs showed an increasing trend, WUE increased first and then decreased slowly, while Ci decreased linearly and then reached a steady state. 4) Maximum carboxylation rate (Vcmax), maximum rate of electron transport (Jmax), triose-phosphate utilization efficiency (TPU), Pnmax, initial carboxylation efficiency (CE) and photorespiration rate (RP) under salinity treatment were lower than those under control, while CO2 saturation point (Cisat) and CO2 compensation point (Γ) showed an opposite trend. 5) Tr and Gs decreased with the increase of CO2 concentration, while WUE and Ci presented linear increase. [Conclusions] The increase of CO2 concentration promotes the increase of Pn, Pnmax, AQY, LSP, Rd, WUE and Ci. Higher CO2 concentration in the environment can increase the photosynthetic efficiency of H. littoralis leaves, especially when the light intensity is high. The salt stress can reduce the photosynthetic rate of H. littoralis leaves significantly. The results of this research indicate that the increase of CO2 concentration promotes the growth of H. littoralis seedling, while salinity treatment inhibits its growth.
肖石红, 张卫强, 黄芳芳, 甘先华, 蔡坚, 朱子维. CO2加富对施盐处理下银叶树幼苗叶片光合特性的影响[J]. 中国水土保持科学, 2020, 18(5): 127-135.
XIAO Shihong, ZHANG Weiqiang, HUANG Fangfang, GAN Xianhua, CAI Jian, ZHU Ziwei. Effect of CO2 enrichment on photosynthetic characteristics in the leaves of Heritiera littoralis seedlings under salinity treatment. SSWC, 2020, 18(5): 127-135.
段利民,闫雪,刘廷玺,等.多重情景下半干旱地区人工杨树林光合生理特征[J].水土保持学报,2019,33(1):215. DUAN Limin, YAN Xue, LIU Tingxi, et al. Photosynthetic physiological characteristics of artificial populus in semi-arid area under multiple scenarios[J]. Journal of Soil and Water Conservation, 2019, 33(1):215.
[2]
TOLEDO-ACEVES T, SWAINE M D.Above-and below-ground competition between the liana acacia kamerunensis and tree seedlings in contrasting light environments[J].Plant Ecology,2008,196(2):233.
[3]
BOWES G.Growth at elevated CO2:photosynthetic responses mediated through Rubisco[J].Plant Cell and Environment,2006,14 (8):795.
[4]
关军锋,李广敏.施磷对限水灌溉小麦根冠及产量的影响研究[J].中国生态农业学报,2004,12 (4):102. GUAN Junfeng, LI Guangmin. Effects of application on root-top characteristics and yield of wheat under water-limited condition[J]. Chinese Journal of Eco-Agriculture,2004,12(4):102.
[5]
刘晓聪,董家华,欧英娟,等.大气中CO2与O3浓度升高对植物光合作用影响研究[J].环境科学与管理,2016,41(3):152. LIU Xiaocong, DONG Jiahua, OU Yingjuan. Research progress on effects of elevated atmospheric O3 and CO2 on plant photosynthesis[J]. Environmental Science and Management, 2016,41(3):152.
[6]
JIANG Yanling,XU Zhenzhu,ZHOU Guangsheng,et al.Elevated CO2 can modify the response to a water status gradient in a steppe grass:From cell organelles to photosynthetic capacity to plant growth[J].BMC Plant Biology,2016,16(1):157.
[7]
毕玉伟,秦俊,胡永红,等.CO2摩尔分数增高对盆栽香樟光合特性及生长量的影响[J].东北林业大学学报,2011,39 (5):27. BI Yuwei, QIN Jun, HU Yonghong, et al. Effects of elevated carbon dioxide concentration on photosynthetic characteristics and growth of Cinnamomum camphora seedlings[J]. Journal of Northeast Forestry University, 2011,39(5):27.
[8]
PORRAS M E, LORENZO P, MEDRANO E, et al.Photosynthetic acclimation to elevated CO2 concentration in a sweet pepper (Capsicum annuum) crop under Mediterranean greenhouse conditions:Influence of the nitrogen source and salinity[J].Functional Plant Biology,2017,44(6):573.
[9]
王伯荪,张炜银,昝启杰,等.红树植物之诠释[J].中山大学学报(自然科学版),2003,42(3):42. WANG Bosun, ZHANG Weiyin, ZAN Qijie, et al. Annotation of mangrove plant[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni,2003,42(3):42.
[10]
陈晓霞,李瑜,茹正忠,等.深圳坝光银叶树群落结构与多样性[J].生态学杂志,2015,34(6):1487. CHEN Xiaoxia, LI Yu, RU Zhengzhong, et al. Community structure and diversity of Heritiera littoralis in Baguang of Shenzhen[J]. Chinese Journal of Ecology, 2015, 34(6):1487.
[11]
简曙光,韦强,唐恬,等.深圳盐灶银叶树种群的生物学特性研究[J].华南农业大学学报,2005,26 (4):84. JIAN Shuguang, WWI Qiang, TANG Tian, et al. Biological characteristics of Heritiera littoralis population in Yanzao, Shenzhen[J]. Journal of South China Agricultural University, 2005, 26(4):84.
[12]
CHRISTOPHER R, NYANDORO S S, CHACHA M, et al.A new cinnamoylglycoflavonoid,antimycobacterial and antioxidant constituents from Heritiera littoralis leaf extracts[J].Natural Product Research,2014,28(6):351.
[13]
YE Yong, LU Changyi, WONG Y S, et al.Diaspore traits and inter-tidal zonation of non-viviparous mangrove species[J].Acta Botanica Sinica,2004,46(8):896.
[14]
MANGORA M M, MTOLERA M, BJÖRK M.Effects of waterlogging,salinity and light on the productivity of Bruguiera gymnorrhiza and Heritiera littoralis seedlings[J].African Journal of Marine Science,2017,39(2):167.
[15]
JIAN Shuguang, TANG Tian, ZHONG Yang, et al.Conservation genetics of Heritiera littoralis (Sterculiaceae),a threatened mangrove in China,based on AFLP and ISSR markers.[J].Biochemical Systematics and Ecology,2010,38 (5):924.
[16]
叶子飘.光合作用对光和CO2响应模型的研究进展[J].植物生态学报,2010,34 (6):727. YE Zipiao. A review on modeling of responses of photosynthesis to light and CO2[J]. Chinese Journal of Plant Ecology, 2010,34 (6):727.
[17]
LONG S P, BERNACCHI C J.Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error[J].Journal of Experimental Botany,2003,54 (392):2393.
[18]
周丹丹,刘德玺,李存华,等.盐胁迫对朴树和速生白榆幼苗光合特性及叶绿素荧光参数的影响[J].西北植物学报,2016,36 (5):1004. ZHOU Dandan, LI Dexi, LI Cunhua, et al. Photosynthetic characteristics and chlorophyll fluorescence parameters of Celtis sinensis and Ulmus pumila L. seedling under salt stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2016,36(5):1004.
[19]
厉书豪,李曼,张文东,等.CO2加富对盐胁迫下黄瓜幼苗叶片光合特性及活性氧代谢的影响[J].生态学报,2019,39(6):249. LI Shuhao, LI Man, ZHANG Wendong, et al. Effects of CO2 enrichment on photosynthetic characteristics and reactive oxygen species metabolism in leaves of cucumber seedlings under salt stress[J]. Acta Ecologica Sinica,2019,39(6):249.
[20]
李远航,贺康宁,张潭,等.盐胁迫对黑果枸杞光合生理指标的影响[J].中国水土保持科学,2019,17 (1):82. LI Yuanhang, HE Kangning, ZHANG Tan, et al. Effects of salt stress on the photosynthetic and physiological indexes of Lycium ruthenicum[J]. Science of Soil and Water Conservation, 2019,17(1):82.
[21]
惠红霞,许兴,李守明.盐胁迫抑制枸杞光合作用的可能机理[J].生态学杂志,2004,23(1):5. HUI Hongxia, XU Xing, LI Shouming. Possible mechanism of inhibition on photosynthesis of Lycium barbarum under salt stress[J]. Chinese Journal of Ecology, 2004, 23(1):5.
[22]
ALBERT K R, RO-POULSEN H, MIKKELSEN T N, et al.Interactive effects of elevated CO2, warming, and drought on photosynthesis of Deschampsia flexuosa in a temperate heath ecosystem[J].Journal of Experimental Botany,2011,62(12):4253.
[23]
叶子飘,张海利,黄宗安,等.叶片光能利用效率和水分利用效率对光响应的模型构建[J].植物生理学报,2017,53 (6):1116. YE Zipiao, ZHANG Haili, HUANG Zongan, et al. Model construction of light use efficiency and water use efficiency based on a photosynthetic mechanistic model of light response[J]. Plant Physiology Journal, 2017, 53(6):1116.
[24]
王建林,温学发,赵风华,等.CO2浓度倍增对8种作物叶片光合作用、蒸腾作用和水分利用效率的影响[J].植物生态学报,2012,36 (5):438. WANG Jianlin, WEN Xuefa, ZHAO Fenghua, et al. Effects of doubled CO2 concentration on leaf photosynthesis, transpiration and water use efficiency of eight crop species[J]. Chinese Journal of Plant Ecology, 2012,36(5):438.
[25]
郑云普,李菲,侯毅凯,等.大气CO2浓度增加对作物光合性能及叶片水分利用效率的影响[J].农业工程学报,2019,35(10):91. ZHENG Yunpu, LI Fei, HOU Yikai, et al. Effect of increasing CO2 concentration on photosynthesis and leaf water use efficiency of crops[J]. Transactions of the CSAE, 2019,35(10):91.
[26]
叶子飘,段世华,安婷,等.C4作物电子传递速率对CO2响应模型的构建及应用[J].植物生态学报,2018,42(10):1000. YE Zipiao, DUAN Shihua, AN Ting, et al. Construction of CO2-response model of electron transport rate in C4 crop and its application[J]. Chinese Journal of Plant Ecology, 2018,42(10):1000.
[27]
王海珍,韩路,徐雅丽,等.胡杨异形叶光合作用对光强与CO2浓度的响应[J].植物生态学报,2014,38(10):1099. WANG Haizhen, HAN Lu, XU Yali, et al. Photosynthetic responses of the heteromorphic leaves in Populus euphratica to light intensity and CO2 concentration[J]. Chinese Journal of Plant Ecology, 2014,38(10):1099.
2020, Vol. 18 
