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A preliminary study of fecal microbiota transplantation alleviating inhibition of radiation-induced hippocampal neurogenesis in mice
Li Junyan, Zhang Junjun, Tian Ye
Department of Radiotherapy Oncology, the Second Affiliated Hospital of Soochow University, Institute of Radiotherapy Oncology, Soochow University, Suzhou Key Laboratory for Radiation Oncology, Suzhou 215004, China
AbstractObjective To evaluate the effects of whole brain irradiation (WBI) and fecal microbiota transplantation (FMT) on hippocampal neurogenesis and the composition of gut microbiota in mice. Methods Forty specific pathogen free ICR male mice (8-week-old, weighed 30 g) were divided into four groups by simple random sample method: control group (group C), radiation group (group R), group C+FMT and group R+FMT, 10 in each group. Animal models were established by WBI at a dose of 10 Gy by 4 MeV electron beam. In group C+FMT and group R+FMT, mice were gavaged with normal fecal bacteria suspension on day 2 post-irradiation, while those in group C and group R were gavaged with phosphate buffered saline as alternative. Hippocampal tissues and feces in four groups were collected on day 15 post-irradiation. 16S rRNA sequencing was used to detect the species and abundance of fecal flora. BrdU+/NeuN+ immunofluorescence staining was performed to observe the neurogenesis in hippocampus of mice. Results WBI and FMT had no effect on survival rate and body weight of mice. WBI induced the inhibition of hippocampal neurogenesis and flora disorder. The quantity of Bacteroideae and Rumen bacteria was increased by 28.6% and 102.9%, whereas that of Lactobacillus was significantly decreased by 70.6% (P<0.05). FMT regulated the abundance of bacteria. The abundance of Enterobacteriaceae was significantly declined by 65.1% (P=0.028), while that of Lactobacillus was increased by 58.2% (P=0.015). FMT also promoted hippocampal neurogenesis to some extent after WBI. Conclusions This preliminary study demonstrates that FMT alleviates the inhibition of hippocampal neurogenesis and flora disorder induced by WBI in mice. Ionizing radiation directly acting on the whole brain of mice indirectly disturbs the composition of gut microbiota, which in turn affects the degree of hippocampal neurogenesis in the brain of mice. There is a bidirectional interaction between gut microbiota and brain.
Fund:; Fund programs: National Natural Science Foundation of China (81773223, 82102824); Jiangsu Provincial Key Project (BE2018657); Jiangsu Medical Innovation Team Class A (CXDT-37); 2021 Suzhou Science and Technology Development Plan (Medical and Health Science and Technology Innovation-Application Basic Research, SKJY2021081)
Corresponding Authors:
Tian Ye, Email: dryetian@126.com
Cite this article:
Li Junyan,Zhang Junjun,Tian Ye. A preliminary study of fecal microbiota transplantation alleviating inhibition of radiation-induced hippocampal neurogenesis in mice[J]. Chinese Journal of Radiation Oncology, 2023, 32(2): 152-158.
Li Junyan,Zhang Junjun,Tian Ye. A preliminary study of fecal microbiota transplantation alleviating inhibition of radiation-induced hippocampal neurogenesis in mice[J]. Chinese Journal of Radiation Oncology, 2023, 32(2): 152-158.
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