下调NEK-2表达对人肺癌A549细胞放射敏感性影响

doi:10.3760/cma.j.issn.1004-4221.2019.02.010

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Abstract Objective To investigate the influence of down-regulation NEK-2 level on the radiosensitivity of A549 cells. Methods NEK-2 siRNA was transfected to A549 cells with liposome and NEK-2 expression level was inspected by Western blot. The radiosensitivity was detected by clone formation experiment. Cell cycle and cell apoptosis were analyzed by flow cytometry. Immunofluorescence experiment was used to detect the DNA double strand break and repair. Results NEK-2 siRNA successfully suppressed NEK-2 expression in A549 cells and resuced the cell proliferation ability after irradiation compared to the blank control group and the negative control group. It can improve the radiosensitivity of A549 cells (The radiosensitivity of A549 cells enhanced significantly.).The D0 values declined form 1.80 Gy to 1.40 Gy,and the sensitizing enhancement ratio was 1.32.After irradiation,compared to negative control group,the apoptosis rate was significantly improved (7.85% to 17.17%),cells in G2/M phase were obviously increased (9.23% to 30.16%),the DNA double strand break rate was increased (100% to 165%) and the DNA damage repair rate was reduced (100% to 48%) in NEK-2 siRNA group. The comparisons among the groups wer statistically significant (P<0.05). Conclusions NEK-2 siRNA reduced the proliferation and increased the radiosensitivity of A549 cell line,probably by affecting the cell cycle,promoting cell apoptosis and suppressing DNA damage repair.

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	Articles by authors
	Chen Chenglong
	Peng Yiru
	Zhang Nan
	Xue Lian
	Yu Dong

Key words： Cell proliferation Apoptosis Cell cycle DNA repair Radiotherapy sensitization

Received: 20 March 2018

Corresponding Authors: Yu Dong, Email:yudong@suda.edu.cn;Xue Lian, Email:xuelian@suda.edu.cn

Cite this article:

Chen Chenglong,Peng Yiru,Zhang Nan et al. Effect of down-regulation NEK-2 level on radio-sensitibity of A549 cells[J]. Chinese Journal of Radiation Oncology, 2019, 28(2): 131-135.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2019.02.010 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2019/V28/I2/131

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