GOLPH3对食管癌细胞系增殖凋亡及放射敏感性影响研究

doi:10.3760/cma.j.issn.1004-4221.2019.08.011

Abstract
Figure/Table
References()
Related Citation (15)
Read Tracking
Download Tracking

Download: PDF (0 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS) Supporting Info

Abstract

Objective To evaluate the effect of GOLPH3 on the proliferation, apoptosis and radiosensitivity of OE33 esophageal cancer cell line. Methods The expression levels of GOLPH3 mRNA and protein in the esophageal cancer cells and normal esophageal epithelial cells were detected by qRT-PCR and Western blot, respectively. The OE33 esophageal cancer cells were transfected with GOLPH3 siRNA and subject to irradiation treatment simultaneously. The cell proliferation was detected by MTT assay. The cell apoptosis was detected by flow cytometry. The radiosensitivity was assessed by cell cloning test. The expression levels of cleaved Caspase-3, Bax and cleaved Caspase-9 protein levels were quantitatively measured by Western blot. Results The expression levels of GOLPH3 mRNA and protein in the esophageal cancer cells were significantly higher than those in the normal esophageal epithelial cells (both P<0.05). GOLPH3 siRNA could obviously down-regulate the expression levels of GOLPH3 mRNA and protein in the OE33 esophageal cancer cells. The proliferation activity of esophageal cancer cells was decreased, whereas the apoptosis rate was increased and the expression levels of cleaved Caspase-3, Bax and cleaved Caspase-9 were up-regulated after down-regulating the expression of GOLPH3 or irradiation treatment (all P<0.05). After down-regulating the expression of GOLPH3 in the esophageal cancer cells treated with irradiation, the cell proliferation activity was more significantly decreased, whereas the apoptosis rate was elevated and the expression levels of cleaved Caspase-3, Bax and cleaved Caspase-9 were more evidently up-regulated (all P<0.05). In the irradiated OE33 esophageal cancer cells after down-regulating the expression of GOLPH³, the radiosensitization ratio of the cells was 1.673. Conclusions GOLPH3 is highly expressed in the esophageal cancer cells. Down-regulating the expression of GOLPH3 can increase the radiosensitivity, induce the apoptosis and inhibit the proliferation of OE33 esophageal cancer cells.

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Liu Yang
	Jiang Yue
	Yang Yuanyuan
	Liu Xiao
	Xu Wencai

Key words： Esophageal cancer cell line Apoptosis Radiosensitivity GOLPH3 gene

Received: 01 December 2018

Fund:

Key Science and Technology Project of Department of Science and Technology of Henan Province (142102310502);General Science and Technology Research Project of Health Commission of Henan Province (201403026);Science and Technology Research Guiding Plan of Health Commission of Henan Province (201304058)

Cite this article:

Liu Yang,Jiang Yue,Yang Yuanyuan et al. Experimental study of the effect of GOLPH3 on proliferation, apoptosis and radiosensitivity of OE33 esophageal cancer cell line[J]. Chinese Journal of Radiation Oncology, 2019, 28(8): 606-609.

URL:

http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2019.08.011 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2019/V28/I8/606

[1] Steffen A,Schulze MB,Pischon T,et al. Anthropometry and esophageal cancer risk in the European prospective investigation into cancer and nutrition[J]. Int J Cancer,2015,137(3):646-657. DOI:10.1158/1055-9965. EPI-09-0265.
[2] Zhou Z,Zhen C,Bai W,et al. Salvage radiotherapy in patients with local recurrent esophageal cancer after radical radiochemotherapy[J]. Radiat Oncol,2015,10(1):1-7. DOI:10.1186/s13014-015-0358-z.
[3] Wang R,Ke ZF,Wang F,et al. GOLPH3 Overexpression is closely correlated with poor prognosis in human non-small cell lung cancer and mediates its metastasis through upregulating MMP-2 and MMP-9[J]. Cell Phys Biochem,2015,35(3):969-982. DOI:10.1159/000369753.
[4] Hu BS,Hu H,Zhu CY,et al. Overexpression of GOLPH3 is associated with poor clinical outcome in gastric cancer[J]. TumourBiol,2015,30(2):171-175. DOI:10.1007/s13277-012-0576-z.
[5] Li W,Guo F,Gu M,et al. Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer[J]. Journal of Cancer,2015,6(5):420-429. DOI:10.7150/jca.11228.
[6] Wang JH,Chen XT,Wen ZS,et al. High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis[J]. Plos One,2012,7(10):e45622. DOI:10.1371/journal.pone.0045622.
[7] Hu BS,Hu H,Zhu CY,et al. Overexpression of GOLPH3 is associated with poor clinical outcome in gastric cancer[J]. Tumour Biol,2013,34(1):515-520. DOI:10.1007/s13277-012-0576-z.
[8] Zhou J,Xu T,Qin R,et al. Overexpression of Golgi phosphoprotein-3(GOLPH3) in glioblastoma multiforme is associated with worse prognosis[J]. J Neuro-oncol,2012,110(2):195-203. DOI:10.1007/s11060-012-0970-9.
[9] Zhou X,Zhan W,Bian W,et al. GOLPH3 regulates the migration and invasion of glioma cells though RhoA[J]. Biochem Biophys Res Commun,2013,433(3):338-344. DOI:10.1016/j.bbrc.2013.03.003.
[10] Li H,Guo L,Chen SW,et al. GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer[J]. J Translat Med,2012,10(1):168-175. DOI:10.1186/1479-5876-10-168.
[11] Zeng Z,Lin H,Zhao X,et al. Overexpression of GOLPH3 promotes proliferation and tumorigenicity in breast cancer via suppression of the FOXO1 transcription factor[J]. Clin Cancer Res,2012,18(15):4059-4069. DOI:10.1158/1078-0432. CCR-11-3156.
[12] 廖和和,李俊海,王凯斌,等. GOLPH3蛋白在食管癌中的表达及临床意义[J]. 现代肿瘤医学,2014(9):2112-2114. DOI:10.3969/j.issn.1672-4992.2014.09.33.
Liao HH,Li JH,Wang KB,et al. Expression and clinical significance of GOLPH3 protein in esophageal carcinoma[J]. Mod Oncol Med,2014(9):2112-2114. DOI:10.3969/j.issn.1672-4992.2014.09.33.
[13] Shi Y,Han Y,Xie F,et al. ASPP2 enhances oxaliplatin (L-OHP)-induced colorectal cancer cell apoptosis in a p53-independent manner by inhibiting cell autophagy[J]. J Cell Mol Med,2015,19(3):535-543. DOI:10.1111/jcmm.12435.
[14] Wang M,Lu X,Dong X,et al.pERK1/2 silencing sensitizes pancreatic cancer BXPC-3 cell to gemcitabine-induced apoptosis via regulating Bax and Bcl-2 expression[J]. World J Surg Oncol,2015,13(1):1-8. DOI:10.1186/s12957-015-0451-7.
[15] Jiang H,Zhang L,Liu J,et al. Knockdown of zinc finger protein X-linked inhibits prostate cancer cell proliferation and induces apoptosis by activating caspase-3 and caspase-9[J]. Cancer Gene Ther,2012,19(10):684-689. DOI:10.1038/cgt.2012.53.
[16] Liu H,Zhou Y,Tang L. Caffeine induces sustained apoptosis of human gastric cancer cells by activating the caspase 9/caspase 3 signalling pathway[J]. Mol Med Rep,2017,16(3):2445-2454. DOI:10.3892/mmr.2017.6894.
[17] Zumbr gel FK,Machtens DA,Curth U,et al. Survivin does not influence the anti-apoptotic action of XIAP on caspase-9[J]. Biochem Biophys Res Commun,2017,482(4):530-535. DOI:10.1016/j.bbrc.2016.11.094.
[18] Chen B,Wang G,Li W,et al. Memantine Attenuates Cell Apoptosis by Suppressing the Calpain-Caspase-3 Pathway in an Experimental Model of Ischemic Stroke[J]. Exp Cell Res,2017,351(2):163-172. DOI:10.1016/j.yexcr.2016.12.028.
[19] 谭宏伟,楚光华,胡春艳,等. GOLPH3沉默对顺铂诱导的人上皮性卵巢癌A2780/DDP细胞凋亡的影响[J]. 现代肿瘤医学,2016,24(17):2700-2704. DOI:10.3969/j.issn.1672-4992.2016.17.009.
Tan HW,Chu GH,Hu CY,et al. Effect of GOLPH3 silence on apoptosis of A2780/DDP cells induced by cisplatin in human epithelial ovarian cancer[J]. Mod Oncol Med,2016,24(17):2700-2704. DOI:10.3969/j.issn.1672-4992.2016.17.009.