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Preventive effect of Chinese traditional medicine-Qing-Xue granula on radiation induced lung injury in mice
WANG Xiao-zhen, JI Wei, JIANG Heng, ZHAO Lu-jun, YANG Wei-zhi, YANG Yu-fei, WANG Lv-hua
Department of Radiation Oncology, Cancer Hospital (Insititute), Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100021, China Corresponding author:WANG L-hua,Email:wlhwq@yahoo.com
Abstract Objective To evaluate whether oral administration of Chinese tradiational medicine, Qing-Xue granula, can prevent mouse lung injury caused by thoracic radiation. Methods 128 BalB/C mice were divided into 4 groups:control (C) group;radiation (R) group;radiation plus high dose Qing-Xue granula (H) group and radiation plus median dose Qing-Xue granula (M) group. The H and M groups were fed 0.64 g and 0.32 g of Qing-Xue granula dissolved in 0.5 ml saline once daily for two months, which were 4 and 2 times of human dosage, respectively. Whole thorax radiation of 12 Gy was delivered with a single ventral-dorsal field with 6 MV X-ray. Group C and group R received 21 days of 0.5 ml saline feeding. Mice were sacrificed at 1, 2, 4 or 6 months after radiation. Macrophage cell count of lung lavage fluid and hydroxyproline content of left lung were assayed, and the lung fibrosis was scorred according to the Ashcroft′s criteria. The plasma interleukin-6(IL-6) and vascular endothelial growth factor (VEGF) concentration were assayed with ELISA method. The One-way ANOVA was used to test the significance of any differences between groups at each time point. Results The macrophage cell number of lung lavage fluid was significantly lower in the 1 st month in group M than in group R (2∶4,q=3.92,P<0.05), but had no significant difference between group M and C (1∶4,q=2.13, P>0.05). The hydroxyproline content of group H was significantlylower than group R in the 1st and 6th months (q=3.62,3.54,all P<0.05), butstill higher than group C (q=4.09,3.72,all P<0.05). The fibrosis score of group H was significantlylower than group R in the 2nd, 4th and 6th months (q=3.38－4.16, all P<0.05). The IL-6 concentration of group H was significantly lower than group R in the 1st month (q=3.53, P<0.05), but not significantly higher than group C (q=1.41,P>0.05). The VEGF concentration was significantlyhigherin group R than group C since the 2nd month (q=3.12－3.78,P<0.05). The VEGF concentration was significantlyhigherin group H and M than group R in the 2nd and 6th months (q=3.08－3.92,all P<0.05). Conclusions Oral Chinese traditional medicine, Qing-Xue granula, could prevent radiation induced lung fibrosis in mice, especially at high dosage. The degree of elevation of VEGF in plasma was not parallel with that of lung fibrosis.
WANG Xiao-zhen,JI Wei,JIANG Heng et al. Preventive effect of Chinese traditional medicine-Qing-Xue granula on radiation induced lung injury in mice[J]. Chinese Journal of Radiation Oncology, 2012, 21(3): 289-292.
WANG Xiao-zhen,JI Wei,JIANG Heng et al. Preventive effect of Chinese traditional medicine-Qing-Xue granula on radiation induced lung injury in mice[J]. Chinese Journal of Radiation Oncology, 2012, 21(3): 289-292.
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2012, Vol. 21 
