基于CT参数分析模型评估辐射诱导小鼠肺纤维化剂量-效应关系研究

doi:10.3760/cma.j.issn.1004-4221.2019.08.010

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Abstract

Objective To investigate the radiation induced pulmonary fibrosis with a dose-response mouse model, based on the CT image changes of pulmonary fibrosis.Methods Female C57BL6 mice aged 8-10 weeks were randomly divided into 20 Gy or escalated doses of X-ray whole thoracic irradiation (WTI) groups.CT scan was performed at different time points before and after radiation. The average lung density and lung volume changes were obtained by three-dimensional segmentation algorithm. After gene chip and pathological validation,the parameters of CT scan were subject to the establishment of logistic regression model. Results At the endpoint of 24 weeks post-irradiation,the lung density in the 20 Gy irradiation group was (-289.81±12.06) HU,significantly increased compared with (-377.97±6.24) HU in the control group (P<0.001). The lung volume was (0.66±0.01) cm³ in the control group,significantly larger than (0.44±0.03) cm³ in the irradiated mice (P<0.001). The results of quantitative imaging analysis were in accordance with the findings of HE and Mason staining,which were positively correlated with the fibrosis-related biomarkers at the transcriptional level (all R²=0.75,all P<0.001). The ED50 for increased lung density was found to be (13.64±0.14) Gy (R²=0.99,P<0.001) and (16.17±4.36) Gy (R²=0.89,P<0.001) for decreased lung volume according to the logistic regression model. Conclusions Quantitative CT measurement of lung density and volume are reliable imaging parameters to evaluate the degree of radiation-induced pulmonary fibrosis in mouse models. The dose-response mouse models with pulmonary fibrosis changes can provide experimental basis for comparative analysis of high-dose hypofractioned irradiation-and half-lung irradiation-induced pulmonary fibrosis.

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	Articles by authors
	Zhou Meijuan
	Zhou Zhaoming
	Wen Lei
	Liu Hao
	Cao Liji
	Lu Shun
	Li Ziyao
	Hui Zhouguang
	Cai Linbo
	Chen Ming
	Chen Longhua
	Zhou Cheng

Key words： Radiation-induced lung fibrosis Radiological assessment Radiobiology Dose-response effect Mouse

Received: 09 August 2018

Fund:

Youth Project of National Natural Science Foundation of China (81703166)

Corresponding Authors: Zhou Cheng,Email:zhou.rob@gmail.com

Cite this article:

Zhou Meijuan,Zhou Zhaoming,Wen Lei et al. Dose-response relationship of radiation-induced pulmonary fibrosis in mouse models based on CT-derived parameters[J]. Chinese Journal of Radiation Oncology, 2019, 28(8): 601-605.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2019.08.010 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2019/V28/I8/601

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