lncRNA SNHG6靶向miR‐485‐3p对宫颈癌SiHa细胞增殖及放疗敏感性的影响

doi:10.3760/cma.j.cn113030-20210714-00264

摘要
图/表
参考文献
相关文章 (15)
点击分布统计
下载分布统计

全文: PDF (0 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS) 背景资料

摘要目的探讨长链非编码RNA（lncRNA）SNHG6对宫颈癌SiHa细胞增殖和放疗敏感性的影响及其潜在的作用机制。方法检测lncRNA SNHG6、miR‐485‐3p在宫颈癌组织、癌旁组织、SiHa细胞及X线照射的SiHa细胞中的表达。分析lncRNA SNHG6与miR‐485‐3p之间的关系。过表达或敲降SNHG6、miR‐485‐3p后,MTT、Transwell迁移实验和流式细胞仪检测细胞增殖能力、侵袭数目和凋亡率。分析miR‐485‐3p对Wnt/β‐catenin通路的影响以及XAV939对SiHa细胞增殖及放疗敏感性的影响。结果 lncRNA SNHG6在宫颈癌组织和SiHa细胞中高表达,在X线照射的SiHa细胞中低表达,miR‐485‐3p在宫颈癌组织和SiHa细胞中低表达,在X线照射的SiHa细胞中高表达。lncRNA SNHG6靶向miR‐485‐3p。下调lncRNA SNHG6表达抑制了细胞增殖和侵袭,增强了其对X线的放疗敏感性,而miR‐485‐3p抑制剂转染细胞后则起到相反作用。上调lncRNA SNHG6通过miR‐485‐3p促进了SiHa细胞的增殖与侵袭,降低了放疗敏感性。下调miR‐485‐3p激活了Wnt/β‐catenin通路,促进了SiHa的细胞增殖和侵袭,降低了其对X线的放疗敏感性。结论过表达lncRNA SNHG6靶向miR‐485‐3p激活Wnt/β‐catenin通路调控SiHa细胞的增殖及其放疗敏感性。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	张冬丽
	孙桂霞
	田君
	刘俊才

关键词 ： lncRNA SNHG6, miR‐485‐3p, 宫颈肿瘤, 增殖, 放疗敏感性

Abstract：Objective To investigate the effect of lncRNA SNHG6 on the proliferation and radiotherapy sensitivity of cervical cancer SiHa cells and its potential mechanism. Methods The expression levels of lncRNA SNHG6 and miR‐485‐3p in cervical cancer tissues, paracancer tissues, SiHa cells and SiHa cells exposed to X‐ray were detected. The relationship between lncRNA SNHG6 and miR‐485‐3p was analyzed. After overexpression or knockdown of SNHG6 and miR‐485‐3p, cell proliferation ability, number of invasion and apoptosis rate were determined by MTT, Transwell chamber assay and flow cytometry, respectively. The effect of miR‐485‐3p on the Wnt/β‐catenin pathway and the effect of XAV939 on SiHa cell proliferation and radiation sensitivity were analyzed. Results lncRNA SNHG6 was highly expressed in cervical cancer tissues and SiHa cells, whereas was lowly expressed in X‐ray irradiated SiHa cells. miR‐485‐3p was lowly expressed in cervical cancer tissues and SiHa cells, whereas was highly expressed in X‐ray irradiated SiHa cells. lncRNA SNHG6 targeted miR‐485‐3p. Down‐regulation of lncRNA SNHG6 expression inhibited cell proliferation and invasion, and enhanced its sensitivity to X‐ray radiotherapy, while miR‐485‐3p inhibitor transfected cells exerted the opposite effect. The up‐regulation of lncRNA SNHG6 promoted the proliferation and invasion of SiHa cells through miR‐485‐3p, and reduced the sensitivity of radiotherapy. Down‐regulation of miR‐485‐3p activated the Wnt/β‐catenin pathway, promoted cell proliferation and invasion of SiHa, and reduced its radiation sensitivity to X‐ray. Conclusion Overexpression of lncRNA SNHG6 targeting miR‐485‐3p activates the Wnt/β‐catenin pathway to regulate the proliferation and radiotherapy sensitivity of SiHa cells.

Key words： LncRNA SNHG6 MiR‐485‐3p Uterine cervical neoplasms Proliferation Radiosensitivity

收稿日期: 2021-07-14

基金资助:河南省医学科技公关计划联合共建项目（LHGJ20210574）; 河南省2018年科技发展计划项目（182102311175）

通讯作者: 刘俊才,Email：yjk20060702@163.com

引用本文:

张冬丽,孙桂霞,田君等. lncRNA SNHG6靶向miR‐485‐3p对宫颈癌SiHa细胞增殖及放疗敏感性的影响[J]. 中华放射肿瘤学杂志, 2022, 31(9): 828-833.

Zhang Dongli,Sun Guixia,Tian Jun et al. Effect of lncRNA SNHG6 targeting miR‐485‐3p on proliferation and radiotherapy sensitivity of cervical cancer SiHa cells[J]. Chinese Journal of Radiation Oncology, 2022, 31(9): 828-833.

链接本文:

http://journal12.magtechjournal.com/Jweb_fszlx/CN/10.3760/cma.j.cn113030-20210714-00264 或 http://journal12.magtechjournal.com/Jweb_fszlx/CN/Y2022/V31/I9/828

[1] 朱月峰, 王国平, 张亚丽, 等. 早期宫颈癌腹腔镜手术治疗进展及反思[J]. 长治医学院学报, 2019, 33(2): 155‐160. DOI: 10.3969/j.issn.1006‐0588.2019.02.021.
Zhu YF, Wang GP, Zhang YL, et al. Progress and review of laparoscopic surgery for early cervical cancer[J]. J Changzhi Med College, 2019,144(02): 79‐84. DOI: 10.3969/j.issn.1006‐0588.2019.02.021.
[2] 王黎明, 汪辉. 宫颈癌化疗策略及进展[J]. 中国实用妇科与产科杂志, 2019, 35(10): 1095‐1098. DOI: 10.19538/j.fk2019100106.
Wang LM, Wang H. Strategy and progress of chemotherapy for cervical cancer[J].Chin J Pract Gynecol Obstetrics,2019,35(10):1095‐1098. DOI: 10.19538/j.fk2019100106.
[3] 周高英, 潘育翔, 张慧杰, 等. 长链非编码RNAs在肿瘤外周血中的研究进展[J]. 现代肿瘤医学, 2017, 25(21): 3541‐3544. DOI: 10.3969/j.issn.1672‐4992.2017.21.044.
Zhou GY, Pan YX, Zhang HJ, et al. Research progress of long non‐coding RNAs in tumor tissues and peripheral blood[J].J Modern Oncol,2017,25(21):3541‐3544. DOI: 10.3969/j.issn.1672‐4992.2017.21.044.
[4] Ding YM, Chan EC, Liu LC, et al. Long noncoding RNAs: important participants and potential therapeutic targets for myocardial ischaemia reperfusion injury[J]. Clin Exp Pharmacol Physiol, 2020, 47(11):1783‐1790. DOI: 10.1111/1440‐1681.13375.
[5] Chen M, Liu P, Chen Y, et al.Long noncoding RNA FAM201A mediates the radiosensitivity of esophageal squamous cell cancer by regulating ATM and mTOR expression via miR‐101[J]. Front Genet, 2018, 9:611. DOI: 10.3389/fgene.2018.00611.
[6] Ge X, Gu Y, Li D, et al. Knockdown of lncRNA PCAT1 enhances radiosensitivity of cervical cancer by regulating miR‐128/GOLM1 axis[J]. Onco Targets Ther, 2020, 13:10373‐10385. DOI: 10.2147/OTT.S263728.
[7] Chen L, Ren P, Zhang Y, et al. Long non?coding RNA GAS5 increases the radiosensitivity of A549 cells through interaction with the miR?21/PTEN/Akt axis[J]. Oncol Rep, 2020, 43(3):897‐907. DOI: 10.3892/or.2020.7467.
[8] Cai G, Zhu Q, Yuan L, et al. LncRNA SNHG6 acts as a prognostic factor to regulate cell proliferation in glioma through targeting p21[J]. Biomed Pharmacother, 2018, 102:452‐457. DOI: 10.1016/j.biopha.2018.03.083.
[9] Zhou H, Xiong Y, Peng L, et al. LncRNA‐cCSC1 modulates cancer stem cell properties in colorectal cancer via activation of the hedgehog signaling pathway[J]. J Cell Biochem, 2020, 121(3):2510‐2524. DOI: 10.1002/jcb.29473.
[10] Chen M, Xia Z, Chen C, et al. lncRNA MALAT1 promotes epithelial‐to‐mesenchymal transition of esophageal cancer through Ezh2‐Notch1 signaling pathway[J]. Anticancer Drugs, 2018,29(8): 767‐773.DOI: 10.1097/CAD. 0000000000000645.
[11] Li HJ, Sun XM, Li ZK, et al. LncRNA UCA1 promotes mitochondrial function of bladder cancer via the miR‐195/ARL2 signaling pathway[J]. Cell Physiol Biochem, 2017, 43(6):2548‐2561. DOI: 10.1159/000484507.
[12] Wang X, Lai Q, He J, et al. lncRNA SNHG6 promotes proliferation, invasion and migration in colorectal cancer cells by activating TGF‐β/Smad signaling pathway via targeting UPF1 and inducing EMT via regulation of ZEB1[J]. Int J Med Sci, 2019,16(1): 51‐59.DOI: 10.7150/ijms. 27359.
[13] Yan K, Tian J, Shi W, et al. LncRNA SNHG6 is associated with poor prognosis of gastric cancer and promotes cell proliferation and EMT through epigenetically silencing p27 and sponging miR‐101‐3p[J]. Cell Physiol Biochem, 2017, 42(3):999‐1012. DOI: 10.1159/000478682.
[14] Zhang Y, An J, Pei Y. LncRNA SNHG6 promotes LMO3 expression by sponging miR‐543 in glioma[J]. Mol Cell Biochem, 2020, 472(1‐2):9‐17. DOI: 10.1007/s11010‐020‐03772‐0.
[15] Bi X, Guo XH, Mo BY, et al. LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast‐like synoviocytes by sponging miRNA‐4701‐5p in rheumatoid arthritis[J]. EBioMedicine, 2019, 50:408‐420. DOI: 10.1016/j.ebiom.2019.11.024.
[16] Ma J, Kong FF, Yang D, et al. LncRNA miR210HG promotes the progression of endometrial cancer by sponging miR‐337‐3p/137 via the HMGA2‐TGF‐β/Wnt pathway[J]. Mol Ther Nucleic Acids, 2021, 24:905‐922. DOI: 10.1016/j.omtn.2021.04.011.
[17] Zhang Z, Jiang W, Hu M, et al. MiR‐486‐3p promotes osteogenic differentiation of BMSC by targeting CTNNBIP1 and activating the Wnt/β‐catenin pathway[J]. Biochem Biophys Res Commun, 2021,8(566): 59‐66. DOI: 10.1016/j. bbrc. 2021.05.098.
[18] Ni Y, Lu C, Wang W, et al. CircBANP promotes colorectal cancer growth and metastasis via sponging let‐7d‐5p to modulate HMGA1/Wnt/β‐catenin signaling[J]. Mol Ther Oncolytics, 2021, 21:119‐133. DOI: 10.1016/j.omto.2021.03.012.