Abstract：Objective To investigate the effect of lncRNA SNHG6 on the proliferation and radiotherapy sensitivity of cervical cancer SiHa cells and its potential mechanism. Methods The expression levels of lncRNA SNHG6 and miR‐485‐3p in cervical cancer tissues, paracancer tissues, SiHa cells and SiHa cells exposed to X‐ray were detected. The relationship between lncRNA SNHG6 and miR‐485‐3p was analyzed. After overexpression or knockdown of SNHG6 and miR‐485‐3p, cell proliferation ability, number of invasion and apoptosis rate were determined by MTT, Transwell chamber assay and flow cytometry, respectively. The effect of miR‐485‐3p on the Wnt/β‐catenin pathway and the effect of XAV939 on SiHa cell proliferation and radiation sensitivity were analyzed. Results lncRNA SNHG6 was highly expressed in cervical cancer tissues and SiHa cells, whereas was lowly expressed in X‐ray irradiated SiHa cells. miR‐485‐3p was lowly expressed in cervical cancer tissues and SiHa cells, whereas was highly expressed in X‐ray irradiated SiHa cells. lncRNA SNHG6 targeted miR‐485‐3p. Down‐regulation of lncRNA SNHG6 expression inhibited cell proliferation and invasion, and enhanced its sensitivity to X‐ray radiotherapy, while miR‐485‐3p inhibitor transfected cells exerted the opposite effect. The up‐regulation of lncRNA SNHG6 promoted the proliferation and invasion of SiHa cells through miR‐485‐3p, and reduced the sensitivity of radiotherapy. Down‐regulation of miR‐485‐3p activated the Wnt/β‐catenin pathway, promoted cell proliferation and invasion of SiHa, and reduced its radiation sensitivity to X‐ray. Conclusion Overexpression of lncRNA SNHG6 targeting miR‐485‐3p activates the Wnt/β‐catenin pathway to regulate the proliferation and radiotherapy sensitivity of SiHa cells.
Zhang Dongli,Sun Guixia,Tian Jun et al. Effect of lncRNA SNHG6 targeting miR‐485‐3p on proliferation and radiotherapy sensitivity of cervical cancer SiHa cells[J]. Chinese Journal of Radiation Oncology, 2022, 31(9): 828-833.
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