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沉默LncRNA HOXD-AS1调控miR-454-3p表达增加H460细胞放射敏感性
彭秋凤1, 叶奎2, 高静珍1, 邢爱民1
1天津市第四中心医院呼吸二科 300140; 2天津市第四中心医院血管外科 300140
Silencing LncRNA HOXD-AS1 enhances the radiosensitivity of H460 cells by regulating miR-454-3p expression
Peng Qiufeng1,Ye Kui2,Gao Jingzhen1,Xing Aimin1
1Two Families of Respiration,Tianjin Fourth Central Hospital,Tianjin 300140,China; 2Vascular Surgery,Tianjin Fourth Central Hospital,Tianjin 300140,China
Abstract：Objective To explore the effect of LncRNA HOXD-AS1 on the radiosensitivity of H460 cells by targeting the regulation of miR-454-3p expression. Methods H460 cells were irradiated with 0,2,4,6 and 8 Gy,and the expression levels of HOXD-AS1 and miR-454-3p were detected by qRT-PCR. Cell apoptosis rate were measured by flow cytometry experiments. Cell cloning experiments were used to detect cell radiosensitivity. The protein expression levels of Caspase-3,Cyclin D1 and γ-H2AX were detected by Western blot. Results The expression of HOXD-AS1 in H460 cells was significantly increased after X-ray irradiation (P<0.05),while the expression of miR-454-3p was significantly decreased (P<0.05). Silencing HOXD-AS1 significantly promoted apoptosis,increased radiosensitivity of lung cancer cells,promoted Caspase-3,γ-H2AX protein expression,and inhibited Cyclin D1 expression. HOXD-AS1 could target the expression of miR-454-3p. Conclusion Silencing HOXD-AS1 can promote the apoptosis of lung cancer cells and inhibit cell survival by targeting miR-454-3p to increase the radiosensitivity of lung cancer cells.
Peng Qiufeng,Ye Kui,Gao Jingzhen et al. Silencing LncRNA HOXD-AS1 enhances the radiosensitivity of H460 cells by regulating miR-454-3p expression[J]. Chinese Journal of Radiation Oncology, 2019, 28(8): 610-611.
[1] Bussink J,van der Kogel AJ,Kaanders JH. Activation of the PI3-K/AKT pathway and implications for radioresistance mechanisms in head and neck cancer[J]. Lancet Oncol,2008,9(3):288-296. DOI:10.1016/S1470-2045(08)70073-1. [2] Jin C,Lin T,Shan L. Downregulation of Calbindin 1 by miR-454-3p Suppresses Cell Proliferation in Nonsmall Cell Lung Cancer In Vitro[J]. Cancer Biother Radiopharm,2019,34(2):119-127. DOI:10.1089/cbr.2018.2598. [3] Zhao X,Li X,Zhou L,et al. LncRNA HOXA11-AS drives cisplatin resistance of human LUAD cells via modulating miR-454-3p/Stat3[J]. Cancer Sci,2018,109(10):3068-3079. DOI:10.1111/cas.13764.
2019, Vol. 28 
