中药清血颗粒对放射性肺损伤预防作用的实验研究

doi:10.3760/cma.j.issn.1004-4221.2012.03.028

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摘要目的检验中药清血颗粒对小鼠正常肺组织放射性损伤的预防作用。方法 128只BalB/C小鼠随机分为空白对照组(C)、单纯照射组(R)、照射加大剂量中药组(H,成人4倍)、照射加中剂量中药组(M,成人2倍)。清血颗粒制备成1.28 g/ml溶液,口服1 次/d(H组0.64 g/次,M组0.32 g/次),2个月内周一至周五给药。全肺用6 MV X线12 Gy照射,照后24 h给药,C组和R组给等体积生理盐水。放疗后第1、2、4、6个月分别处死 6~8只鼠,采集右肺肺泡灌洗液、左肺、肝素抗凝心脏血于－70℃冻存待测。观察指标包括肺泡灌洗液巨嗜细胞计数、左全肺羟脯氨酸含量测定、肺组织纤维化评分、血管内皮细胞生长因子(VEGF)等含量测定。采用方差分析检验不同时间点各组上述指标差异。结果 M组第1个月肺泡灌洗液巨嗜细胞计数明显低于R组(2∶4,q=3.92,P<0.05),与C组相似(1∶4,q=2.13,P>0.05),H组与R组也相似(2∶3,q=0.82~2.46,P均>0.05)。羟脯氨酸含量H、M、R组各时间点均高于C组(q=3.28~4.36,P均<0.05),H组照射后第1、6个月显著低于R组(q=3.62、3.54,P均<0.05)。肺纤维化评分H组第2、4、6个月均低于R组(q=3.38~4.16,P均<0.05)。第2个月开始R组小鼠VEGF值高于C组(1∶2,q=3.12~3.78,P<0.05),第2个月H、M组高于R组(q=3.58、3.92,P均<0.05);第6个月的也不同(q=3.08~3.76,P均<0.05)。结论 VEGF水平的升高与放射性肺纤维化严重程度不一致。清血颗粒的预防应用可减少照射后小鼠肺组织羟脯氨酸含量、降低肺纤维化评分,从而对放射性肺纤维化有一定预防作用且在一定范围内有剂量依赖性。

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	王小震
	姬巍
	蒋恒
	赵路军
	杨伟志
	杨宇飞
	王绿化

关键词 ：放射性肺损伤, 中药疗法, 小鼠

Abstract：Objective To evaluate whether oral administration of Chinese tradiational medicine, Qing-Xue granula, can prevent mouse lung injury caused by thoracic radiation. Methods 128 BalB/C mice were divided into 4 groups:control (C) group;radiation (R) group;radiation plus high dose Qing-Xue granula (H) group and radiation plus median dose Qing-Xue granula (M) group. The H and M groups were fed 0.64 g and 0.32 g of Qing-Xue granula dissolved in 0.5 ml saline once daily for two months, which were 4 and 2 times of human dosage, respectively. Whole thorax radiation of 12 Gy was delivered with a single ventral-dorsal field with 6 MV X-ray. Group C and group R received 21 days of 0.5 ml saline feeding. Mice were sacrificed at 1, 2, 4 or 6 months after radiation. Macrophage cell count of lung lavage fluid and hydroxyproline content of left lung were assayed, and the lung fibrosis was scorred according to the Ashcroft′s criteria. The plasma interleukin-6(IL-6) and vascular endothelial growth factor (VEGF) concentration were assayed with ELISA method. The One-way ANOVA was used to test the significance of any differences between groups at each time point. Results The macrophage cell number of lung lavage fluid was significantly lower in the 1^st month in group M than in group R (2∶4,q=3.92,P<0.05), but had no significant difference between group M and C (1∶4,q=2.13, P>0.05). The hydroxyproline content of group H was significantlylower than group R in the 1^st and 6^th months (q=3.62,3.54,all P<0.05), butstill higher than group C (q=4.09,3.72,all P<0.05). The fibrosis score of group H was significantlylower than group R in the 2^nd, 4^th and 6^th months (q=3.38－4.16, all P<0.05). The IL-6 concentration of group H was significantly lower than group R in the 1^st month (q=3.53, P<0.05), but not significantly higher than group C (q=1.41,P>0.05). The VEGF concentration was significantlyhigherin group R than group C since the 2^nd month (q=3.12－3.78,P<0.05). The VEGF concentration was significantlyhigherin group H and M than group R in the 2^nd and 6^th months (q=3.08－3.92,all P<0.05). Conclusions
Oral Chinese traditional medicine, Qing-Xue granula, could prevent radiation induced lung fibrosis in mice, especially at high dosage. The degree of elevation of VEGF in plasma was not parallel with that of lung fibrosis.

Key words： Radiation induced lung injury Chinese traditional medicine treatment Mouse

收稿日期: 2011-05-10

引用本文:

王小震,姬巍,蒋恒等. 中药清血颗粒对放射性肺损伤预防作用的实验研究[J]. 中华放射肿瘤学杂志, 2012, 21(3): 289-292.

WANG Xiao-zhen,JI Wei,JIANG Heng et al. Preventive effect of Chinese traditional medicine-Qing-Xue granula on radiation induced lung injury in mice[J]. Chinese Journal of Radiation Oncology, 2012, 21(3): 289-292.

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