Establishment and identification of C57BL/6 mouse model with radiation‐induced pulmonary fibrosis
Yuan Meng1, Men Yu2, Sun Xin1,3, Zhao Maoyuan1,4, Bao Dan5, Yang Xu1, Sun Shuang1, Bao Yongxing1, Ma Zeliang1, Liu Yunsong1, Hui Zhouguang2
1Department of Radiation Oncology, National Cancer Center / National Clinical Research Center for Cancer / Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China; 2Department of VIP Medical Services, National Cancer Center / National Clinical Research Center for Cancer / Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China; 3Department of Radiation Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; 4Lung Cancer Center, West China Hospital of Sichuan University, Chengdu 610041, China; 5Department of Radiology, National Cancer Center / National Clinical Research Center for Cancer / Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Abstract：Objective To establish the mouse model with radiation‐induced pulmonary fibrosis, and to identify and analyze it from the aspects of function, imaging and pathology. Methods Thirty C57BL/6 mice were randomly divided into the control group, 16 Gy irradiation group and 20Gy irradiation group. The mice in the irradiation groups received a single 16 Gy or 20 Gy chest X‐ray irradiation, and underwent functional examination, imaging examination and pathological examination at 3 and 6 months after irradiation. Results At 6 months after irradiation, hair on the chest and back of the mice turned white and fell off, and the airway resistance was increased significantly. CT images showed extensive patch shadows and consolidation in the lung. Three dimensional reconstruction suggested that the lung of mice was distorted and deformed, and the volume was decreased significantly. Pathological examination confirmed that there was extensive pulmonary fibrosis. Conclusions Significant pulmonary fibrosis occurs after 6 months of chest irradiation in mice. The animal model of radiation‐induced pulmonary fibrosis in C57BL/6 mice was successfully established.
Yuan Meng,Men Yu,Sun Xin et al. Establishment and identification of C57BL/6 mouse model with radiation‐induced pulmonary fibrosis[J]. Chinese Journal of Radiation Oncology, 2022, 31(10): 928-932.
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