Establishment of C57BL/6 mouse models with radiation-induced cardiopulmonary dysfunction
Yan Rui1, Song Jianbo2, Cai Honghong3, Guo Min4, Xu Xianhai3, Zhang Yarong3, Yu Yang3, Li Sijin3
1Nursing College of Shaanxi Medical University, Taiyuan 030001, China; 2Department of Radiotherapy of Shaanxi Bethune Hospital (Shaanxi Academy of Medical Sciences),Taiyuan 030032, China; 3Department of Nuclear Medicine, First Hospital of Shaanxi Medical University, Taiyuan 030001, China; 4Department of Cardiology, First Hospital of Shaanxi Medical University, Taiyuan 030001, China
Abstract：Objective To establish the C57BL/6 mouse models of radiation-induced cardiopulmonary dysfunction. Methods Twenty-four male C57BL/6 mice were randomly divided into the control and irradiation groups. Mice in the irradiation group were irradiated with 20 Gy electron beam and bred for 6 months after irradiation. Cardiac function was assessed using ultrasonography. The partial pressure of oxygen was detected by blood gas analysis. Cell apoptosis was observed by Tunel assay. Myocardial and pulmonary fibrosis was assessed by Masson staining. Results The LVEF in the irradiation group was (68.60±10.92)%, significantly less compared with (81.75±8.79)% in the control group (P< 0.01). The apoptotic index of heart in the irraiation group was (23.90±6.60)%, considerably higher than (3.25±3.38)% in the control group (P< 0.01). The CVF of heart in the irradiation group was (15.42±5.72)%, significantly higher than (1.45±0.64)% in the control group (P< 0.01). The PaO2 level in the irradiation group was (86.10±7.60) mmHg, significantly lower compared with (107.16±9.01) mmHg in the control group (P< 0.01). The apoptotic index of lung in the irradiation group was (27.90±8.94)%, significantly higher than (2.50±3.55)% in the control group (P<0.01). The CVF of lung in the irradiation group was (17.76±5.77)%, remarkably higher than (2.50±3.55)% in the control group (P< 0.01). Conclusion Radiation can induce cardiopulmonary apotosis and fibrosis remodeling, which leads to cardiopulmonary dysfunction, suggesting the successful establishment of C57BL/6 mouse model of radiation-induced cardiopulmonary dysfunction.
Yan Rui,Song Jianbo,Cai Honghong et al. Establishment of C57BL/6 mouse models with radiation-induced cardiopulmonary dysfunction[J]. Chinese Journal of Radiation Oncology, 2020, 29(9): 796-798.
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