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17-AAG-M诱导人NSCLC-A549细胞差异表达miRNA的筛选及对放射敏感性影响
张楠1, 薛莲2, 于冬1
1苏州大学医学部放射医学与防护学院 215000; 2苏州大学医学部公共卫生学院 215000
Screening of differentially expressed miRNAs in A549 cells induced by 17-AAG-M and its effect on radio-sensitivity
Zhang Nan1, Xue Lian2, Yu Dong1
1School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215000, China; 2School of Public Health, Medical College of Soochow University, Suzhou 215000, China
Abstract：Objective To screen 17-AAG-M-induced differentially expressed miRNAs in human non-small cell lung cancer (NSCLC) A549 cells under X-ray and evaluate its effect on radio-sensitivity. Methods A549 cells were treated with 17-AAG-M and 4Gy. Total RNA was extracted for microarray screening. The expression of the miRNAs of interest in the tumor was observed by public database. The target miRNAs were analyzed by using GO and KEGG pathways, and verified by qPCR. The effect of target miRNAs on the survival rate and proliferation of A549 cells under X-ray was evaluated by MTT and clone formation assays. The radio-sensitivity of the target miRNAs was analyzed by the single-hit multi-target model formula. Results 20 differentially expressed miRNAs were screened. The down-regulated hsa-miR-30a-3p showed a close correlation with lung cancer in the database. It was involved with 50 biological processes including cell proliferation and affected the MAPK signaling pathway, cancer-related pathways and cell cycle, etc. Compared with the 17-AAG-M group, the relative expression level of hsa-miR-30a-3p under the action of 17-AAG-M and X-ray was down-regulated from 2.42 to 0.16. hsa-miR-30a-3p inhibited the survival rate of A549 cells (survival rate:78.52%) and further decreased to 69.00% under X-ray. Up-regulation of hsa-miR-30a-3p expression inhibited the proliferation of tumor cells and increased the radio-sensitivity of A549 cells. The radio-sensitization ratio was 1.18. The above performance became more obvious under the action of 17-AAG-M. Conclusions In A549 cells, hsa-miR-30a-3p is differentially expressed under the action of 17-AAG-M and X-ray. Moreover, up-regulation of the expression level of hsa-miR-30a-3p in A549 cells can reduce the viability and proliferation of tumor cells, and increase the radio-sensitivity of tumor cells. The inhibition effect of X-ray combined with 17-AAG-M upon tumors can be strengthened.
Zhang Nan,Xue Lian,Yu Dong. Screening of differentially expressed miRNAs in A549 cells induced by 17-AAG-M and its effect on radio-sensitivity[J]. Chinese Journal of Radiation Oncology, 2021, 30(6): 608-613.
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2021, Vol. 30 
