Effect of miR-133b on radiosensitivity of colon cancer SW620 cells by targeting HER-2
Zhao Lei1, Wang Tianyu1, Wang Xiaomin2, Liu Junzhong1, Yi Shanyong3
1Department of Intervention, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China; 2Department of Radiation, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China; 3Depatment of Oncology, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China
Abstract：Objective To evaluate the effect of miR-133b on the apoptosis and radiosensitivity of colon cancer cell line (SW620 cells), and to explore its mechanism. Methods SW620 cells were transfected with miR-con (miR-con group), miR-133b mimics (miR-133b group), si-con (si-con group) and si-HER-2(si-HER-2 group) by the liposome method, and then irradiated with 0, 2, 4, 6, 8 Gy. The miR-133b protein expression, HER-2 protein expression, apoptosis, cell survival fraction and cytofluoroactivity in each group were evaluated by qRT-PCR, Western blot, flow cytometry, colony formation assay and dual luciferase reporter gene assay, respectively. Results Compared with the pre-irradiation group, the expression level of miR-133b was significantly down-regulated (P<0.05), whereas that of HER-2 was significantly up-regulated in SW620 cells after irradiation (P<0.05). Overexpression of miR-133b and knockdown of HER-2 remarkably reduced the survival fraction (both P<0.05),and significantly promoted the apoptosis of SW620 cells (P<0.05). miR-133b could considerably inhibit the fluorescent activity of wild-type HER-2 cells (P<0.05) and negatively regulate the expression of HER-2 protein. Conclusion miR-133b can inhibit the survival of colon cancer cells, promote the apoptosis and enhance the sensitivity of radiotherapy probably via the mechanism of targeting HER-2.
Zhao Lei,Wang Tianyu,Wang Xiaomin et al. Effect of miR-133b on radiosensitivity of colon cancer SW620 cells by targeting HER-2[J]. Chinese Journal of Radiation Oncology, 2021, 30(4): 403-406.
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