Abstract：A micro-propagation system for tetraploid black locust ( Robinia pseudoacacia ) was established by using its shoots. The optimum medium for shoot multiplication and growth was MS + BA0.5 mg·L-1 + NAA0. 1 mg·L-1 . The best medium for rooting was 1/2 MS + IBA 0.4 mg·L-1 + NAA0.25 mg·L-1. The adventitious root development of shoots in test tube was also observed by means of the anatomical method. Latent root primordium was not found in shoots in test tubes. The induced root primordium which originated from the division and differentiation of pith ray cell developed into the adventitious roots. It had been proved that survival percentage had been increased by means of the plantlets acclimated through leaf anatomical observation.
李云；王树芝；田砚亭；罗晓芳. 四倍体刺槐离体培养及其不定根发育和叶片解剖观察[J]. 中国水土保持科学, 2003, 1(1): 91-94.
LI Yun-；Wang-Shu-Zhi-；Tian-Yan-Ting-；Luo-Xiao-Fang. Study on Tissue Culture of Tetraploid Black Locus Clone and Anatomical Observation on the Development of Adventitious Roots and Structure of Leaves. SSWCC, 2003, 1(1): 91-94.
2003, Vol. 1 
