Abstract:[Background] Soil microbiomes play a crucial role in the overall health and stability of the desert ecosystem. Understanding how engineering construction influences the soil microbial communities is crucial for effective land management and sustainable development in desert regions.However, the mechanismunderlying thisinfluence remains unclear. [Methods] We investigated deep soil bacterial communities (60-80 cm) beneath the transmission tower base, which has been built for two years, in the western Gonghe Basin of Qinghai province. High-throughput amplicon sequencingwas performed to explore bacterial communities (16S rRNA). Bioinformatics analyses were also deployed, including Principal Coordinates Analysis (PCoA), Mantel, and null model analysis. [Results] The results revealed a significanteffect of tower base construction on the deep soil ecosystem. Chemical properties (soil microbial carbon, microbial nitrogen, organic carbon, total nitrogen, total phosphorus, total potassium, available phosphorus, available potassium) and α-diversity (Chao1 and phylogenetic diversity) significantly decreased after the tower base construction. Actinobacteria, Acidobacteria, and Proteobacteria dominated in deep soils, accounting for 50.08% of the total abundance. In particular, beneathtransmission tower, the abundance of anaerobic bacterial phyla such as Actinobacteria, Firmicutes, and Chloroflexi decreased significantly. Moreover, PCoAanalysis revealed that the bacterial community structure beneath the transmission towers had a dramatic difference from that in adjacent undisturbed lands. Null model analysisshowed that stochastic processes primarily influenced the assembly of bacterial communities in deep soils, bacterial communities beneath transmission towers demonstrated a notable increase in the randomness (|βNTI| > 2: 21.1%). Mantel test indicated thatkey driving factors of bacterial diversity and community structure in the deep soils were identified as pH, total nitrogen, and available phosphorus. The construction of tower bases disrupted the soil’s physical structure, resulting in changes in soil chemical properties, which in turn impacted the composition and structure of the soil bacterial community. [Conclusions] This study increases our understanding of the ramifications of human activities, particularly engineering construction and production, on the biotic environment of deep soil. The findings highlight the importance of sustainable practices in construction and land management. Furthermore, the study provides valuable theoretical support for soil and water conservation and ecological restoration of desert ecosystems.
王文昌, 鲁旺胜, 荆可. 青海共和盆地荒漠区输电塔基建设对深层土壤细菌群落结构的影响[J]. 中国水土保持科学, 2024, 22(5): 161-170.
WANG Wenchang, LU Wangsheng, JING Ke. Effects of transmission line tower base construction on the structure of deep soil bacterial communities. SSWC, 2024, 22(5): 161-170.
MIDDLETON N, THOMASS D. (Eds.). World atlas of desertification [M]. 2nd ed. United Nations Environment Programme,1997: 35.
[2]
NEILSON J W, CALIFF K, CARDONA C. Significant impacts of increasing aridity on the arid soil microbiome [J]. mSystems, 2017, 2(3): 1128.
[3]
MARTÍNEZ-VALDERRAM J, GUIRADO E, MAESTRE F T. Desertifying deserts [J]. Nature Sustainability, 2020, 3(8): 572.
[4]
ZHANG Kaoping, SHI Yu, CUI Xiaoqing, et al. Salinity is a key determinant for soil microbial communities in a desert ecosystem [J]. mSystems, 2019, 4(1): 10.
[5]
DELGADO-BAQUERIZO M, DOULCIER G, ELDRIDGE D J,et al. Increases in aridity lead to drastic shifts in the assembly of dryland complex microbial networks [J]. Land Degradation & Development 2020, 31(3): 346.
[6]
MAESTRE F T, DELGADO-BAQUERIZO M, JEFFRIES T C, et al. Increasing aridity reduces soil microbial diversity and abundance in global drylands [J]. Proceedings of the National Academy Sciences of United States of America, 2015, 112(51): 15684.
[7]
STEVEN B, PHILLIPS M L, BELNAP J, et al. Resistance, resilience, and recovery of dryland soil bacterial communities across multiple disturbances [J]. Frontiers in Microbiology, 2021, 12: 8.
[8]
张力斌, 何明珠, 张克存,等. 干旱风沙区植被重建初期对土壤微生物群落结构的影响[J].干旱区地理, 2022, 45(6): 1916. ZHANG Libin, HE Mingzhu, ZHANG Kecun, et al. Effect of preliminary vegetation reconstruction on soil microorganism community structure in arid desert area [J]. Arid Land Geography. 2022, 45(6):1916.
[9]
WANG Xiaobo, Lü Xiaotao, YAO Jing, et al. Habitat-specific patterns and drivers of bacterial β-diversity in China's drylands [J]. The ISME Journal, 2017, 11(6): 1345.
[10]
COWAN D A, HOPKINS D W, JONES B E, et al. Microbiomes of Namib desert habitats [J]. Extremophiles, 2020, 24(1): 17.
[11]
FISHER K, JEFFERSON J S, VAISHAMPAYAN P. Bacterial communities of Mojave Desert biological soil crusts are shaped by dominant photoautotrophs and the presence of hypolithic niches [J]. Frontiers in Ecology and Evolution, 2020, 7:518.
[12]
SCHULZE-MAKUCH D, WAGNER D, KOUNAVES S P, et al. Transitory microbial habitat in the hyperarid Atacama Desert [J]. Proceedings of the National Academy of Sciences, 2018, 115(11): 2670.
[13]
LEUNG P M, BAY S K, MEIER D V, et al. Energetic basis of microbial growth and persistence in desert ecosystems [J]. mSystems, 2020, 5(2): 1110.
[14]
TRIPATHI B M, STEGEN J C, KIM M, et al. Soil pH mediates the balance between stochastic and deterministic assembly of bacteria [J]. The ISME Journal, 2018, 12(4):1072.
[15]
WU Ying, CHEN Dima, DELGADO-BAQUERIZO M, et al. Long-term regional evidence of the effects of livestock grazing on soil microbial community structure and functions in surface and deep soil layers[J]. Soil Biology and Biochemistry, 2022, 168: 6.
[16]
YANG Xihang, YUE Linyan, WU Ying, et al. The validity of ecological hypotheses concerning aboveground organisms for soil microbial biomass and diversity across soil depths on the Mongolian Plateau[J]. Applied Soil Ecology, 2023, 181: 104679.
[17]
ZHANG Qiufang, QIN Wenkuan, FENG Jiguang, et al. Whole-soil-profile warming does not change microbial carbon use efficiency in surface and deep soils[J]. Proceedings of the National Academy of Sciences, 2023, 120(32): 10.
[18]
洪倩, 陈晓枫, 余蔚青,等.输电线路塔基施工余土的改良及其植物生长试验[J].中国水土保持科学, 2022, 20(4): 126. HONG Qian, CHEN Xiaofeng, YU Weiqing, et al. An experimental study on improvement of residual soil and the plant growth in transmission line tower foundation construction [J]. Science of Soil and Water Conservation, 2022, 20(4):126.
[19]
刘继武, 韩恺, 闫炜炀. 山西采动影响区输电线路塔基区水土流失特点及防治措施[J].山西电力, 2021(5):23. LIU Jiwu, HAN Kai, YAN Weiyang. Study on water and soil erosion characteristics and control measures of transmission line tower base in mining-affected area of Shanxi province[J]. Shanxi Electric Powe, 2021(5):23.
[20]
丰佳, 陈晓刚, 王文龙,等. 云南土石山区输变电线路工程水土流失特征研究[J]. 中国水土保持, 2022(7): 31. FENG jia, CHEN Xiaogang, WANG Wenlong, et al.Soil and water loss characteristics of transmission and transformation line project in Yunnan earth-rock mountain area [J]. Soil and Water Conservation in China, 2022(7): 31.
[21]
韩晓锐. 输电线路涉及环境敏感区的环境影响及污染防治措施研究[J]. 环境与发展, 2019, 31(7): 32. HAN Xiaorui. Study on environmental impacts and pollution prevention measures of transmission lines in environmentally sensitive areas [J]. Environment and Development, 2019, 31(7): 32.
[22]
张曼玉, 王志涛, 邓磊,等. 共和盆地不同灌木群落生物土壤结皮理化性质差异[J]. 干旱区研究, 2023, 40(11): 1797. ZHANG Manyu, WANG Zhitao, DENG Lei, et al. Differences in the physical and chemical properties of biological soil crusts in different shrub communities in the Gonghe Basin [J]. Arid Zone Research, 2023, 40(11):1797.
[23]
王伦, 周鸿玉, 杨秀玲,等. 共和盆地不同恢复年限人工混交林表层土壤微生物群落特征[J]. 青海大学学报, 2023, 41(2): 1. WANG Lun, ZHOU Hongyu, YANG Xiuling, et al. Characteristics of soil microbial communities of artificial mixed forest with different restoration years in Gonghe basin [J]. Journal of Qinghai University, 2023, 41(2): 1.
[24]
CHEN Yongjian, NEILSON J W, KUSHWAHA P, et al. Life-history strategies of soil microbial communities in an arid ecosystem [J]. The ISME Journal, 2021, 15(3): 649.
[25]
MORENO-JIMÉNEZ E, PLAZA C, SAIZ H, et al. Aridity and reduced soil micronutrient availability in global drylands [J]. Nature Sustainability, 2019, 2(5):371.
[26]
YEH Y C, MCNICHOL J, NEEDHAM D M, et al. Comprehensive single-PCR 16S and 18S rRNA community analysis validated with mock communities, and estimation of sequencing bias against 18S [J]. Environmental Microbiology, 2021, 23: 3240.
[27]
CAPORASO J G, LAUBER C L, WALTERS W A, et al. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms [J]. The ISME Journal, 2012, 6(8): 1621.
[28]
周桦, 宇万太, 马强,等. 氯仿薰蒸浸提法测定土壤微生物量碳的改进[J].土壤通报, 2009, 40(1): 154. LU Hua, YU Wanta, MA Qiang, et al. A modified fumigation extraction method for the determination of soil microbial biomass carbon [J]. Chinese Journal of Soil Science, 2009, 40(1): 154.
[29]
鲁如坤.土壤农业化学分析方法[M].北京: 中国农业科技出版社, 2000: 330. LU Rukun. Methods of soil agricultural chemical analysis[M]. Beijing: China Agricultural Science and Technology Press, 2000: 330.
[30]
NAYLOR D, MCCLURE R, JANSSON J. Trends in microbial community composition and function by soil depth [J]. Microorganisms, 2022, 10(3): 540.
[31]
SUI Biao, WANG Li, WANG Hongbin, et al. Deep tillage inhibits microbial species interactions and exhibits contrasting roles in bacterial and fungal assembly [J]. Agriculture, Ecosystems & Environment, 2023, 357: 108679.
[32]
CRǎCIUN N, NEGREA B, POP C E, et al. Experimental research compared aquaculture of certain species of the Lemna genus with demonstration of environmental requirements and of the adaptations to environmental conditions specific to aquatic eutroph-polytroph ecosystems [J]. Scientific Annals of the Danube Delta Institute, 2020, 25: 33.
[33]
KONG Weibo, WEI Xiaorong, WU Yonghong, et al. Afforestation can lower microbial diversity and functionality in deep soil layers in a semiarid region [J]. Global Change Biology, 2022, 28(20): 6086.
[34]
ZHOU Zixuan, WANG Yunqiang. Global patterns of dried soil layers and environmental forcing [J]. Land Degradation & Development, 2023, 11(15): 3364.
[35]
HU Yigang, WANG Zengru, ZHANG Zhishan, et al. Alteration of desert soil microbial community structure in response to agricultural reclamation and abandonment [J]. Catena, 2021, 207: 105678.
[36]
JIAO Shuo, CHU Haiyan, ZHANG Baogang, et al. Linking soil fungi to bacterial community assembly in arid ecosystems [J]. iMeta, 2022, 1(1): 1.
[37]
KU Yongli, HAN Xiaoting, LEI Yuting, et al. Different sensitivities and assembly mechanisms of the root-associated microbial communities of Robinia pseudoacacia to spatial variation at the regional scale [J]. Plant and Soil, 2023, 486: 621.
[38]
JANSSON J K, HOFMOCKL K S. Soil microbiomes and climate change [J]. Nature Reviews Microbiology, 2020, 18(1):35.
[39]
ROUSK J, BÅÅTH E, BROOKES P C, et al. Soil bacterial and fungal communities across a pH gradient in an arable soil [J]. The ISME Journal, 2010, 4(10): 1340.
[40]
PHILIPPOT L, CHENU C, KAPPLER A, et al. The interplay between microbial communities and soil properties [J]. Nature Reviews Microbiology, 2023, 1: 226.
[41]
LI Jiwei, XIE Jianbo, ZHANG Yu, et al. Interactive effects of nitrogen and water addition on soil microbial resource limitation in a temperate desert shrubland [J]. Plant and Soil, 2022, 475(1/2): 361.
[42]
LI Jiabo, SHEN Zehao, LI Chaonan, et al. Stair-step pattern of soil bacterial diversity mainly driven by pH and vegetation types along the elevational gradients of Gongga Mountain, China [J]. Frontiers in Microbiology, 2018, 9: 569.
[43]
YIN Yue, WANG Xiaojie, HU Yuanan, et al. Soil bacterial community structure in the habitats with different levels of heavy metal pollution at an abandoned polymetallic mine [J]. Journal of Hazardous Materials, 2023, 442:130063.
[44]
孙欣. 半干旱草原微生物物种组成和功能基因对降水变化的响应[D]. 北京: 清华大学, 2015:67. SUN Xin. Responses of microbial community taxonomic composition and functional genes to precipitation changes in semi-arid grasslands [D]. Beijing: Tsinghua University, 2015:67.
[45]
SHU Wensheng, HUANG Linan. Microbial diversity in extreme environments [J]. Nature Reviews Microbiology, 2022, 20(4): 219.