A pot experiment was conducted to explore the effects of soil conditioners on soil properties and growth of Juglans regia seedlings, through the determination of soil physical properties and the indexes of growth and physiological on J. regia seedlings with different treatments. The effects of three self-made multifunctional soil conditioners ( I，II ，III) on the growth of J. regia seedlings were compared with an ordinary soil conditioner by using variance analysis and factor analysis on soil physical properties and the physiological indicators. The results showed that the application of soil conditioner could significantly improve soil porosity and soil water holding capacity, decrease soil bulk density, and promote the growth of J. regia seedlings. Soil conditioners had significant influences on soil properties and growth of J.regia seedlings. The effects of self-made multifunctional soil conditioners were better than the ordinary soil conditioner. The length of shoots treated with self-made multifunctional soil conditioner I was 44.1% higher than that of the control group and 36.3% higher than that of the ordinary soil conditioner. Compared with the control group and the ordinary soil conditioner, the ground diameter of self-made multifunctional soil conditioners I increased by 20.4% and 11.6%, and the biomass of self-made multifunctional soil conditioners I increased by 32.3% and 8.2% ,and the photosynthetic rate of self-made multifunctional soil conditioner I increased by 35.6% and 15.1%,while the transpiration rate of self-made multifunctional soil conditioner I decreased by 26.7 % and 15.6%, respectively. Hence, the self-made multifunctional soil conditioner I can be used as the optimal formula. This study had a certain guiding function for exploring soil conditioner of environmental protection composite.