[Background] The metabolism of carbon and nitrogen is critical to tobacco. Organic material is the main source of the nutrient, which directly affects the carbon (C) and nitrogen (N) cycle of tobacco. In this study, we try to find the optimal type of organic material for the C and N cycle of the tobacco and the relevant soil in middle of Henan Province. [Methods] The pot experiment was carried out in the farm of Henan Agricultural University in 2015. Four treatments were set as: 1) no organic material application (TCK ), 2) add maize straw (TS ), 3) add pig manure (TM ), 4) add biochar (TB). Except TCK treatment, the C/ N ratio in TS, TM, and TB treatment were regulated as 25/1. The tobacco leaves and the soil were sampled on the 30, 45, 60, 75 and 90 days after the tobacco transplanted. The major chemical and biological indices such as the content of organic carbon, varied forms of N, phosphors (P), kalium (K), the relative enzyme activities of carbon and nitrogen cycle were measured. [Results] Added biochar and pig manure significantly improved the agronomic traits of tobacco, while, the opposite effect on tobacco was appeared under maize straw treatment. The activities of key enzymes related to C and N metabolism in the tobacco leaves were significantly promoted under biochar treatment. The highest activities of nitrate reductase, amylase, and invertase were 33.3 µg/ (g·h), 14.42 mg/ (g·min), and 5.08 mg/ (g·h), respectively. Compared to the control (without organic material added), the contents of N, P, and K in tobacco leaf were significantly elevated under the treatment with organic material application. Meanwhile, under the treatments with organic material, the soil urease activity was significantly improved under pig manure treatment, and the maximum value was 1.78 mg/ kg; while the soil sucrase activity changed little among the treatment. The contents of soil organic carbon and soil nitrogen were remarkably increased. Especially, the content of total nitrogen and available nitrogen in pig manure treatment were significantly higher than that in other treatments. The organic material promoted the soil nitrification process, and resulted in higher content of nitrate nitrogen. [Conclusions] Based on the C/ N as 25/1, the organic material can enhance the activities of the enzyme which are related to carbon and nitrogen cycle, adjust the chemical components in the tobacco leaves and improve the content of the nutrition in soil. Biochar application can improve the C and N metabolism and then lead to more harmonious chemical components in the tobacco leaves, and promote the quality of tobacco; while the pig manure provided more available nutrition contents in the soil.