尿素与多效唑配施对苯磺隆胁迫下土壤酶活性的影响

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摘要通过盆栽试验，采用三因素完全随机设计，研究尿素、多效唑配施对苯磺隆胁迫下土壤脲酶、碱性磷酸酶、蛋白酶活性的影响。结果表明：不同浓度苯磺隆对土壤脲酶活性表现为抑制—激活作用，抑制和激活作用均随着苯磺隆浓度的增大而增强，第60d激活率是对照的2.83倍；低浓度苯磺隆对碱性磷酸酶表现为激活—抑制—激活作用，高浓度苯磺隆表现为抑制—激活作用，第60d极显著地高于对照，是对照的2.49倍；低浓度苯磺隆对蛋白酶活性表现为激活—抑制作用，高浓度苯磺隆表现为抑制—激活作用；尿素、多效唑（除B1N1处理对蛋白酶的影响外）配施可减弱苯磺隆对土壤脲酶、碱性磷酸酶和蛋白酶活性的抑制作用，大多数处理表现为抑制—激活作用，在处理结束时（第60d），脲酶和碱性磷酸酶仍极显著地高于对照。

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	吴济南
	王丽玲
	王惟帅
	杜慧玲

关键词 ：苯磺隆, 尿素, 多效唑, 脲酶, 碱性磷酸酶, 蛋白酶

Abstract：A pot experiment was conducted with three-factor completely randomized design to evaluate the dynamic effects of combined application of urea and paclobutrazol on the activity of soil urease, alkaline phosphatase and protease under tribenuron-memthyl stress. The results indicated that: different concentrations of tribenuron-methyl had inhibition-activation effects on soil urease activity, and the effects would be strengthened with increase of concentration. The activation rate on the 60th day was 2.83 times of the control. Alkaline phosphatase activity took on activation-inhibition-activation trend in low concentration of tribenuron-methyl treatment, and inhibition-activation in high concentration treatment. The activation rate on the 60th day was 2.49 times of the control. Protease activity showed activation- inhibition trend in low concentrations of tribenuron-methyl treatment, vice verse, inhibition-activation trend in high concentration treatment. Combined application of paclobutrazol and urea (except B1N1 treatment on protease activity) weakened the inhibition effects on soil urease, alkaline phosphatase and protease activity under tribenuron-methyl stress, and most treatments led to inhibition-activation trend. Urease and alkaline phosphatase activities were significantly higher than the control on the 60th day.

Key words： tribenuron-methyl urea paclobutrazol urease alkaline phosphatase protease

收稿日期: 2011-04-02

基金资助:

山西省科技攻关项目“麦田除草剂苯磺隆残留危害的综合治理技术”；（20110311022）山西农业大学博士基金“除草剂残留的安全性评价及小麦对其响应基因性差异的研究”（412559）；横向委托项目“苯磺隆残留风险评价”（2010HX02）

引用本文:

吴济南，王丽玲，王惟帅，杜慧玲. 尿素与多效唑配施对苯磺隆胁迫下土壤酶活性的影响[J]. 中国水土保持科学, 2011, 9(4): 110-116.
TUN Ji-Na, Wang-Li-Ling, Wang-Wei-Shuai, Du-Hui-Ling. Effect of combined application of urea and paclobutrazol on soil enzyme activity under tribenuron-methyl stress. SSWCC, 2011, 9(4): 110-116.

链接本文:

http://journal12.magtechjournal.com/Jweb_stbc/CN/ 或 http://journal12.magtechjournal.com/Jweb_stbc/CN/Y2011/V9/I4/110