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Effect of hyperthermia on biological behavior of squamous cell carcinoma of the tongue by regulating ferroptosis pathway
Shen Pei1,2, Hao Yuli2, Zhou Xuexiao3, Cong yuan1,2, Wang Shengzhi2, Shao yun1,2, Xu Ting2, Li Shouyi2
1School of Stomatology of Qingdao University, Qingdao 266003, China; 2Department of Stomatology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai 264099, China; 3Department of Dental Implantology, Hospital of Stomatology Sichuan University, Chengdu 610044, China
AbstractObjective To investigate the regulation and possible mechanism of hyperthermia (HT) on the ferroptosis of squamous cell carcinoma of the tongue cell line CAL‐27. Methods Half maximal inhibitory concentration (IC50) of Fer‐1, an inhibitor of ferroptosis, was detected by CCK‐8 assay and used for subsequent experiments. CAL‐27 cells were divided into the HT, control, Fer‐1 and HT+ Fer‐1 groups according to experimental design. Reactive oxygen species (ROS) levels and iron ion concentration were determined by corresponding detection kits. The p53 and TfR1 mRNA levels were detected by real‐time reverse transcription PCR. Cell migration was detected by cell scratch test and cell apoptosis was detected by flow cytometry. Results HT significantly up‐regulated the ROS levels (P<0.01) and iron ion concentration (P<0.001), and significantly increased the expression levels of p53 and TfR1 mRNA (both P<0.01). The cell migration ability was decreased (P<0.001), whereas cell apoptosis rate was increased by HT (P<0.01). In the HT+Fer‐1 group, the ROS levels (P<0.001), iron ion concentration (P<0.001), expression levels of p53 and TfR1 mRNA (both P<0.01) were significantly down‐regulated, the cell migration ability was recovered (P<0.01), and cell apoptosis rate was decreased (P<0.01) compared with those in the HT group, respectively. Conclusions HT may induce the ferroptosis of CAL‐27 cell line, inhibit cell migration ability and promote cell apoptosis by activating the p53/TfR1 pathway.
Fund:Yantai Science and Technology Innovation Development Plan Project (2022YD021)
Corresponding Authors:
Wang Shengzhi, Email: Wangsz916@163.com; Li Shouyi, Email: shouyi516@163.com
Cite this article:
Shen Pei,Hao Yuli,Zhou Xuexiao et al. Effect of hyperthermia on biological behavior of squamous cell carcinoma of the tongue by regulating ferroptosis pathway[J]. Chinese Journal of Radiation Oncology, 2023, 32(3): 260-264.
Shen Pei,Hao Yuli,Zhou Xuexiao et al. Effect of hyperthermia on biological behavior of squamous cell carcinoma of the tongue by regulating ferroptosis pathway[J]. Chinese Journal of Radiation Oncology, 2023, 32(3): 260-264.
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