急性放射性食管炎大鼠动物模型的构建

doi:10.3760/cma.j.cn113030-20220321-00109

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Abstract Objective To establish Wistar rat models of acute radiation esophagitis, and observe the histopathological changes at different time points after modeling. Methods Wistar rats were locally irradiated with different doses of 6 MV X‐rays, and the rats were sacrificed on the 3^rd, 5^th, 7^th, and 14^th days after irradiation. The full‐length esophagus tissue was taken for paraffin embedding, sectioning, and hematoxylin and eosin (HE) staining for pathological assessment. The pathological changes of the esophagus of the rats were observed at the 3^rd, 5^th, 7^th, and 14^th days after 25 Gy and 30 Gy irradiation. The changes of daily dietary intake of rats in different irradiation groups within 1‐2 weeks after radiation exposure were observed. Results No rat died in two groups after being irradiated with 25 Gy and 30 Gy rays. All the rats in the 30 Gy group had esophagus injury. On the 7^th day, the degree of injury was the most serious, with a pathological score of 5.00±0.75 and a food intake of 0 g. On the 14^th day, the degree of injury was relieved, and the food intake was restored to the level before irradiation. Conclusions The Wistar rat model of acute radiation esophagitis can be established by a single dose of 6 MV X‐ray 30 Gy irradiation to the esophagus. The 7^th day after irradiation is an ideal observation time for the acute injury phase, which is gradually alleviated after the 7^th day. The time can be chosen from 7‐14 days after irradiation as the observation point for the healing repair phase.

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	Articles by authors
	Li Kaixuan
	Wen Yanping
	Zheng Jiabin
	Teng Feng
	Jia Liqun

Key words： Rats,Wistar Radiation esophagitis,acute Models,animal

Received: 21 March 2022

Fund:The National Administration of Traditional Chinese Medicine Clinical Collaborative Project of Traditional Chinese and Western Medicine for Major and Intractable Diseases‐Esophageal Cancer (2019‐ZX‐005)

Corresponding Authors: Jia Liqun, Email: liqun‐jia@hotmail.com

Cite this article:

Li Kaixuan,Wen Yanping,Zheng Jiabin et al. Establishment of a rat model of acute radiation esophagitis[J]. Chinese Journal of Radiation Oncology, 2023, 32(3): 254-259.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.cn113030-20220321-00109 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2023/V32/I3/254

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