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Effect of upregulated HuR gene on radiosensitivity of esophageal squamous cell carcinoma cell Kyse450
Han Dan1, Li Lu1, Kan Zhiwen2, Tao Zhenchao3, Qian Liting3
1Department of Life Science and Medicine, University of Science and Technology of China,Hefei 230026,China; 2Anhui Medical University Affiliated Provincial Hospital,Hefei 230022,China; 3Department of Radiotherapy,West District of the First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Cancer Hospital),Hefei 230031,China
AbstractObjective To evaluate the effect of upregulation of HuR gene on the radiosensitivity of esophageal squamous cell carcinoma cell Kyse450. Methods The HuR gene of Kyse450 cells was upregulated by lentivirus. At the same time, X-ray irradiation at a dose of 6Gy was selected as the intervention condition. Western blot and qPCR were used to detect the expression levels of protein and RNA after Kyse450 transfection, respectively. CCK8 kit was employed to determine the cell proliferation rate. Clone formation assay was adopted to evaluate the ability of cell clone formation. Wound healing experiment and the Transwell test were performed to detect changes in cell migration. Results CCK8 assay showed that the proliferation ability of cells was enhanced after upregulation of HuR gene, and this enhancement trend was more obvious after radiation. The plate cloning experiment showed that with the increase of radiation dose, the clone formation rates were decreased in both groups, but the clone formation rate in the overexpression group was higher than that in the control group. Wound healing experiment and Transwell test demonstrated that the wound healing rate and migration ability in the overexpression group were higher than those in the control group, and the difference was more significant after radiotherapy. Western blot showed that the levels of MMP9 and MMP2 at 24h after radiotherapy in the overexpression group were higher than those in the control group. Conclusion The upregulation of HuR can enhance the proliferation, cloning, migration capabilities and decrease the radiosensitivity of esophageal squamous cell carcinoma cells.
Fund:Youth Fund of Anhui Tumor Hospital (2018YJQN012);Research on the Fundamental Theory and Application of Big Data Knowledge Engineering (2016YFB1000905)
Han Dan,Li Lu,Kan Zhiwen et al. Effect of upregulated HuR gene on radiosensitivity of esophageal squamous cell carcinoma cell Kyse450[J]. Chinese Journal of Radiation Oncology, 2022, 31(5): 456-461.
Han Dan,Li Lu,Kan Zhiwen et al. Effect of upregulated HuR gene on radiosensitivity of esophageal squamous cell carcinoma cell Kyse450[J]. Chinese Journal of Radiation Oncology, 2022, 31(5): 456-461.
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