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Effect of hyperthermia combined with paclitaxel on proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27
Zhou Xuexiao1,2, Shen Pei1,2, Shi Fan1,2, Shao Yun1,2, Cong Yuan1,2,Xu Ting2, Wang Shengzhi2
1School of Stomatology of Qingdao University, Qingdao 266003, China; 2Department of Stomatology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai 264000, China
AbstractObjective To observe the effect of hyperthermia combined with paclitaxel on the proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27, and to explore the underlying mechanism. Methods The working concentration of paclitaxel was determined by CCK-8 assay, and the cultured CAL-27 cells were divided into the control, paclitaxel, 42℃ hyperthermia and combined treatment groups. The ability of cell proliferation was detected by colony formation assay, and the cell cycle and apoptosis were determined by flow cytometry. The expression levels of AKT, p-AKT, Bcl-2 and Bax proteins in each group were measured by Western blot. Results Compared with the control group, the proliferation was significantly inhibited and the apoptosis of CAL-27 cells was significantly promoted in the combined treatment, hyperthermia and paclitaxel groups (all P<0.05), and the anti-proliferation and apoptosis-promoting effect in the combined treatment group was significantly better than those in the hyperthermia and paclitaxel groups (all P<0.05). Western blot showed that hyperthermia combined with paclitaxel could significantly up-regulate the expression level of Bax protein and significantly down-regulate the expression levels of P-AKT and Bcl-2 in CAL-27 cells (all P<0.05). Conclusions Hyperthermia combined with paclitaxel can play a synergistic role in inhibiting proliferation and promoting apoptosis of tongue squamous cell carcinoma CAL-27 cells. The mechanism may be related to the inhibition of AKT activation and the activation of Bax/Bcl-2 apoptosis signaling pathway.
Corresponding Authors:
Wang Shengzhi, Email:Wangsz916@163.com
Cite this article:
. Effect of hyperthermia combined with paclitaxel on proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27[J]. Chinese Journal of Radiation Oncology, 2022, 31(2): 192-195.
. Effect of hyperthermia combined with paclitaxel on proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27[J]. Chinese Journal of Radiation Oncology, 2022, 31(2): 192-195.
[1] Liao S, Hu X, Liu Z, et al. Synergistic action of microwave-induced mild hyperthermia and paclitaxel in inducing apoptosis in the human breast cancer cell line MCF-7[J]. Oncol Lett, 2019, 17(1):603-615. DOI:10.3892/ol.2018.9629. [2] Iyikesici MS. Feasibility study of metabolically supported chemotherapy with weekly carboplatin/paclitaxel combined with ketogenic diet, hyperthermia and hyperbaric oxygen therapy in metastatic non-small cell lung cancer[J]. Int J Hyperthermia, 2019, 36(1):446-455. DOI:10.1080/02656736.2019.1589584. [3] Hildebrandt B, Wust P, Ahlers O, et al. The cellular and molecular basis of hyperthermia[J]. Crit Rev Oncol/Hematol, 2002, 43(1):33-56. DOI:10.1016/S1040-8428(01)00179-2. [4] Yang WH, Xie J, Lai ZY, et al. Radiofrequency deep hyperthermia combined with chemotherapy in the treatment of advanced non-small cell lung cancer[J]. Chin Med J (Engl), 2019, 132(8):922-927. DOI:10.1097/CM9.0000000000000156. [5] Amin M, Huang W, Seynhaeve A, et al. Hyperthermia and temperature-sensitive nanomaterials for spatiotemporal drug delivery to solid tumors[J]. Pharmaceutics, 2020, 12(11):1007. DOI:10.3390/pharmaceutics12111007. [6] Ahmed K, Zaidi SF, Mati-Ur-Rehman, et al. Hyperthermia and protein homeostasis:cytoprotection and cell death[J]. J Therm Biol, 2020, 91:102615. DOI:10.1016/j.jtherbio.2020.102615. [7] Becher I, Andrés-Pons A, Romanov N, et al. Pervasive protein thermal stability variation during the cell cycle[J]. Cell, 2018, 173(6):1495-1507, e18. DOI:10.1016/j.cell.2018.03.053. [8] Wu J, Liu T, Rios Z, et al. Heat shock proteins and cancer[J]. TrendsPharmacol Sci, 2017, 38(3):226-256. DOI:10.1016/j.tips.2016.11.009. [9] van Rhoon GC, Franckena M, Ten Hagen T. A moderate thermal dose is sufficient for effective free and TSL based thermochemotherapy[J]. Adv Drug Deliv Rev, 2020,163-164:145-156. DOI:10.1016/j.addr.2020.03.006. [10] Oei AL, Kok HP, Oei SB, et al. Molecular and biological rationale of hyperthermia as radio-and chemosensitizer[J]. Adv Drug Deliv Rev, 2020, 163-164:84-97. DOI:10.1016/j.addr.2020.01.003. [11] Revathidevi S, Munirajan AK. Akt in cancer:mediator and more[J]. Semin Cancer Biol, 2019, 59:80-91. DOI:10.1016/j.semcancer.2019.06.002. [12] Yu F, Sugawara T, Maier CM, et al. Akt/Bad signaling and motor neuron survival after spinal cord injury[J]. Neurobiol Dis, 2005, 20(2):491-499. DOI:10.1016/j.nbd.2005.04.004. [13] Adams JM, Cory S. The Bcl-2 protein family:arbiters of cell survival[J]. Science, 1998, 281(5381):1322-1326. DOI:10.1126/science.281.5381.1322.
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