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Effect of mannose on the radiosensitivity of six non-small cell lung cancer cell lines
Ge Hong1, Luo Hui1, Liu Kangdong2, Jia Xuechao2, Nie Wenna2, Zhang Qiqi2, Lu Bingbing2, Yang Ran2, Wang Nan1, Song Shuai1, Jiao Ruidi1
1Department of Radiation Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China; 2Sino-American Hormel Cancer Institute, Zhengzhou 450003, China
AbstractObjective To investigate the effect of mannose on the radiosensitivity of six human non-small cell lung cancer cell lines and its possible mechanism. Methods The expression of mannose phosphate isomerase in six lung cancer cell lines were detected by Western blot. The inhibitory effect of mannose on the proliferation of lung cancer cell lines were observed by MTT assay. When irradiated with 0, 2, 4, 6, 8 and 10Gy, the effect of mannose on the radiosensitivity of six lung cancer cell lines was detected by plate clone formation assay, respectively;and the apoptosis rates of normal control, mannose, irradiation and combined groups were detected by flow cytometry. Results The expression levels of mannose phosphate isomerase were different among six lung cancer cell lines. Among them, A549 cells had the highest expression level and H460 cells showed the lowest expression level. When aDministrated with 11.1 mmol/L mannose, the same inhibitory effect was observed on both A549 and H460 cell lines. Moreover, the inhibitory effect on H460 cell line was significantly increased with the increase of mannose concentration. In addition, aDministration of 11.1 mmol/L mannose could significantly increase the radiosensitivity and apoptosis rate of H460 cell line. However, it exerted limited effect upon the radiosensitivity and apoptosis rate of A549 cell line. Conclusion In six lung cancer cell lines with high expression of mannose phosphate isomerase, the aDministration of mannose can enhance the radiosensitivity of partial tumors cells.
Fund:National Natural Science Foundation of China (81773230);The Technology Open and Cooperation Program of Henan (182106000062)
Cite this article:
Ge Hong,Luo Hui,Liu Kangdong et al. Effect of mannose on the radiosensitivity of six non-small cell lung cancer cell lines[J]. Chinese Journal of Radiation Oncology, 2020, 29(7): 558-562.
Ge Hong,Luo Hui,Liu Kangdong et al. Effect of mannose on the radiosensitivity of six non-small cell lung cancer cell lines[J]. Chinese Journal of Radiation Oncology, 2020, 29(7): 558-562.
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2020, Vol. 29 
