[an error occurred while processing this directive] | [an error occurred while processing this directive]
Research progress on radioresistance and sensitization of tumor cells in three-dimensional culture system
Gao Jianong1, Liang Shanshan2, Yu Weiting3, Wang Ruoyu1,2
1Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001,China; 2Key Laboratory of High-Throughput Screening or Targeted Drug Transformation of Molecular Markers of Breast and Digestive Tumors in Liaoning Province, Dalian 116001,China; 3Department of Orthopedics Medical Research Center, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001,China;
Abstract Three-dimensional cell culture is a novel type of in vitro culture method. This system can mimic the microenvironment of cell growth in vivo, where cells exhibit similar state and function to that in the in vivo environment. Currently, three-dimensional culture system has been widely applied in tissue engineering and tumor cell biology fields, etc. Radiotherapy is an important treatment of cancer. Radioresistance of tumor cells is the main cause of treatment failure, tumor recurrence and metastasis. Tumor cells can exhibit the resistant characteristics in the three-dimensional culture system, similar to those of tumor cells in vivo. Therefore, three-dimensional culture system can be adopted to investigate the radioresistance and underlying mechanism of tumor cells and identify the key factors regulating the radioresistance of tumor cells, which plays a pivotal role in enhancing the radioresistance and improving the effect of radiotherapy. This article will review recent research progress in the radioresistance and sensitization of tumor cells under three-dimensional culture system, aiming to provide reference for relevant investigations.
Gao Jianong,Liang Shanshan,Yu Weiting et al. Research progress on radioresistance and sensitization of tumor cells in three-dimensional culture system[J]. Chinese Journal of Radiation Oncology, 2019, 28(1): 65-68.
Gao Jianong,Liang Shanshan,Yu Weiting et al. Research progress on radioresistance and sensitization of tumor cells in three-dimensional culture system[J]. Chinese Journal of Radiation Oncology, 2019, 28(1): 65-68.
[1] Qiao H,Tang TT.Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis[J].Bone Res,2018,6(1):12.DOI:10.1038/s41413-018-0008-9. [2] Shamir ER,Ewald AJ.Three-dimensional organotypic culture:experimental models of mammalian biology and disease[J].Nat Rev Mol Cell Biol,2014,15(10):647-664.DOI:10.1038/nrm3873. [3] Carrel A,Ingebrigtsen R.The production of antibodies by tissues living outside of the organism[J].J Exper Med,1912,15(3):287-291.DOI:10.1084/jem.15.3.287. [4] Dart A.Tumour microenvironment microbes matter[J].Nat Rev Cancer,2018,18(1):120-120.DOI:10.1038/nrc.2017.120. [5] Dornier E,Norman JC.Tensin links energy metabolism to extracellular matrix assembly[J].J Cell Biol,2017,216(4):867-869.DOI:10.1083/jcb.201702025. [6] Steglich A,Vehlow A,Eke I,et al.α-Integrin targeting for radiosensitization of three-dimensionally grown human head and neck squamous cell carcinoma cells[J].Cancer Lett,2015,357(2):542-548.DOI:10.1016/j.canlet.2014.12.009. [7] Rubashkin MG,Ou G,Weaver VM.Deconstructing signaling in three dimensions[J].Biochemistry,2014,53(13):2078-2090.DOI:10.1021/bi401710 d. [8] Ahmed KM,Zhang H,Park CC.NF-κB inhibition promotes radiosensitivity of breast cancer cells in three-dimensional culture through abating β1-integrin expression[J].Cancer Research,2011,71:1.DOI:10.1158/0008-5472.sabcs11-p3-01-06. [9] Frenguelli L,Al-Akkad W,Crowley C,et al. Development of human liver extracellular matrix hydrogel for three dimensional cell culture and cell transplantation[J].J Hepatol,2017,66(1):S339-S340.DOI:10.1016/s0168-8278(17)31009-7. [10] Weiswald LB,Bellet D,Dangles-Marie V.Spherical cancer models in tumor biology[J].Neoplasia,2015,17(1):1-15.DOI:10.1016/j.neo.2014.12.004. [11] Durand RE,Sutherland RM.Effects of intercellular contact on repair of radiation damage[J].Experimental Cell Research,1972,71(1):75-80.DOI:10.1016/0014-4827(72)90265-0. [12] Pan D,Chen Y,Du Y,et al. Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells[J].Oncotarget,2017,8(3):4422-4435.DOI:10.18632/oncotarget.12647. [13] Oumano M,Ngwa W,Celli J,et al. Gold nanoparticle-enhanced radiation therapy in in-vitro A549 lung carcinoma:studies in both traditional monolayer and three dimensional cell culture models[J].Med Phys,2016,43(6):3787-3787.DOI:10.1118/1.4957704. [14] Kim JS,Kim SY,Lee M,et al. Radioresistance in a human laryngeal squamous cell carcinoma cell line is associated with DNA methylation changes and topoisomerase Ⅱ alpha[J].Cancer Biol Ther,2015,16(4):558-566.DOI:10.1080/15384047.2015.1017154. [15] Pan D,Chen YX,Du YR,et al. Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells[J].Oncotarget,2017,8(3):4422-4435.DOI:10.18632/oncotarget.12647. [16] Page H,Flood P,Reynaud EG.Three-dimensional tissue cultures:current trends and beyond[J].Cell and Tissue Research,2013,352(1):123-131.DOI:10.1007/s00441-012-1441-5. [17] Xue G,Ren Z,Grabham PW,et al. Reprogramming mediated radio-resistance of 3D-grown cancer cells[J].Journal of Radiation Research,2015,56(4):656-662.DOI:10.1093/jrr/rrv018. [18] Williams KE,Bundred NJ,Landberg G,et al. Focal adhesion kinase and wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy[J].Stem Cells,2015,33(2):327-341.DOI:10.1002/stem.1843. [19] Pan D,Xue G,Zhu J,et al. Ionizing radiation induced biological effects in three-dimensional cell cultures[J].Rend Linc,2013,25(S1):81-86.DOI:10.1007/s12210-013-0260-2. [20] Simon KA,Mosadegh B,Minn KT,et al. Metabolic response of lung cancer cells to radiation in a paper-based 3D cell culture system[J].Biomaterials,2016,95(1):47-59.DOI:10.1016/j.biomaterials.2016.03.002. [21] Dornish JM,Markussen C,Anderson T.The effect of x-ray radiation on spheroids cultured in a 3D matrix under different oxygen conditions[J].Cancer Res,2013,73(8):1.DOI:10.1158/1538-7445.am2013-3846. [22] Bartel C,Tichy A,Schoenkypl S,et al. Effects of steroid hormones on differentiated glandular epithelial and stromal cells in a three dimensional cell culture model of the canine endometrium[J].Bmc Veter Res,2013,9:16.DOI:10.1186/1746-6148-9-86. [23] Balcioglu HE,van Hoorn H,Donato DM,et al. The integrin expression profile modulates orientation and dynamics of force transmission at cell-matrix adhesions[J].J Cell Sci,2015,128(7):1316-1326.DOI:10.1242/jcs.156950. [24] Klapproth E, Dickreuter E, Zakrzewski F, et al. Whole exome sequencing identifies mTOR and KEAP1 as potential targets for radiosensitization of HNSCC cells refractory to EGFR and beta1 integrin inhibition[J]. Oncotarget,2018,9(26):18099-18114. DOI:10.18632/oncotarget.24266. [25] Gomez-Roman N,Stevenson K,Gilmour L,et al. A novel 3D human glioblastoma cell culture system for modeling drug and radiation responses[J].Neuro-Oncology,2017,19(2):229-241.DOI:10.1093/neuonc/now164.
2019, Vol. 28 
