mRNA干扰BMI-1对人食管癌细胞放射增敏作用研究

doi:10.3760/cma.j.issn.1004-4221.2017.06.014

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Abstract Objective To investigate the effects of BMI-1 expression inhibition by RNA interference on the radiosensitivity of esophageal cancer TE-13 cells and its mechanism. Methods The siRNA based on the sequence of BMI-1 mRNA was synthesized to transfect cultured TE-13 cells as BMI-1 siRNA group, a negative one was synthesized to transfect cultured TE-13 cells as negative control group (NC group), and untransfected TE-13 cells were named as control group. The expression of the BMI-1 mRNA and protein in TE-13 cells was measured by quantitative real-time PCR and Western blot, respectively. The cell proliferation and the radiosensitivity of TE-13 cells were measured by MTS and colony-forming assay, respectively. Flow cytometry was used to analyze cell cycle and apoptosis. The expression of BCL-2 and BAX in TE-13 cells was measured by Western blot. Comparison between groups was made by analysis of variance. Results The BMI-1 siRNA group had significantly lower expression of BMI-1 mRNA and protein than the control group and the NC group (P=0.000,0.000). The proliferation of TE-13 cells in the BMI-1 siRNA group decreased significantly after irradiation (P=0.031). The colony-forming assay showed that the BMI-1 siRNA group had a significantly higher radiosensitivity than the control group and the NC group (P=0.000). After irradiation, the BMI-1 siRNA group had a significantly lower percentage of cells in G₂/M phase than the control group and the NC group (P=0.000,0.000). The BMI-1 siRNA group had a significantly increased apoptosis rate (P=0.000,0.000),significantly reduced expression of BCL-2(P=0.000,0.000), and significantly increased expression of BAX after irradiation (P=0.000,0.000). Conclusions BMI-1 siRNA can inhibit the expression of BMI-1 gene in esophageal cancer TE-13 cells, eliminate the cell cycle arrest in G₂/M phase, induce cell apoptosis after ionizing irradiation in vitro, and increase the radiosensitivity, which may be related to the regulation of the expression of BCL-2 and BAX.

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	Articles by authors
	Yang Xingxiao
	Ma Ming
	Song Heng
	Liu Zhikun
	Zhu Shuchai

Key words： BMI-1 gene RNA interference Irradiation Cell cycle Apoptosis

Received: 22 June 2016

Corresponding Authors: Zhu Shuchai,Email:sczhu1965@163.com

Cite this article:

Yang Xingxiao,Ma Ming,Song Heng et al. Effect of BMI-1 on radiosensitization of esophageal carcino-ma cells after silencing of BMI-1 gene[J]. Chinese Journal of Radiation Oncology, 2017, 26(6): 671-676.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2017.06.014 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2017/V26/I6/671

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