慢性间歇性低压低氧预处理可减轻大鼠放射性心脏损伤

doi:10.3760/cma.j.issn.1004-4221.2017.02.020

Abstract
Figure/Table
References()
Related Citation (12)
Read Tracking
Download Tracking

Download: PDF (1230 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS) Supporting Info

Abstract Objective To study cardioprotective effects of pretreatment with chronic intermittent hypobaric hypoxia (CIHH) on acute radiation-induced heart damage, and to explore the potential mechanisms. Methods Forty-eight male adult Sprague-Dawley rats were randomly divided into control group, CIHH group, radiation group, and CIHH+radiation group. Pretreatment with CIHH was performed by placing rats in a hypobaric chamber before radiation. Left ventricular function was recorded and myocardial infarction area was determined. Collagen volume fraction (CVF) was calculated after Masson staining. The activity of total superoxide dismutase (T-SOD) and content of lipid peroxide malondialdehyde (MDA) were determined by enzyme linked immunosorbent assay. The expression levels of a marker of fibrosis, collagen type I (COL-1), and two markers of endoplasmic reticulum stress, GRP78 and CHOP, were analyzed by Western blot. Difference test using two factor analysis of variance. Results Compared with the radiation group, the CIHH+radiation group had improved left ventricular systolic and diastolic function and reduced myocardial infarction area;the CIHH+radiation group also had significantly reduced CVF, significantly lower expression of COL-1, significantly higher activity of T-SOD, significantly lower concentration of MDA, and significantly lower expression levels of GRP78 and CHOP (P<0.01,P<0.01;(185.19±3.20) vs. (156.61±4.60) U/mgprot, P<0.01;(1.36±0.17) vs. (2.36±0.21) nmol/mgprot, P<0.01;P<0.05;P<0.01). Conclusions Pretreatment with CIHH can to a certain degree reduce radiation-induced damage to the cardiac coronary artery, enhance cardiac tolerance to ischemia/reperfusion injury, reduce myocardial infarction area after ischemia/reperfusion, and improve collagen deposition in perivascular region and myocardial stroma. CIHH protects myocardium probably via inhibition of oxidative stress, endoplasmic reticulum stress, and myocardial fibrosis.

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Wu Yajing
	Wang Jun
	Wang Yuan
	Wang Sheng
	Liu Qing
	Wang Yi
	Cao Feng
	Cheng Yunjie
	Jiao Wenpeng

Key words： Chronic intermittent hypobaric hypoxia Radiation induced heart damage Langendorff perfusion Endoplasmic reticulum stress Oxidative stress

Received: 11 July 2016

Corresponding Authors: Wang Jun,Email:wangjunzr@163.com;Wang Sheng,Email:wangsheng@hebmu.edu.cn

Cite this article:

Wu Yajing,Wang Jun,Wang Yuan et al. Pretreatment with chronic intermittent hypobaric hypoxia attenuates radiation-induced heart damage in rats[J]. Chinese Journal of Radiation Oncology, 2017, 26(2): 222-227.

URL:

http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2017.02.020 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2017/V26/I2/222

[1] 王军,武亚晶,王媛,等.X线照射对大鼠心脏缺血再灌注损伤影响[J].中华放射肿瘤学杂志,2016,25(6):640-645.DOI:10.3760/cma.j.issn.1004-4221.2016.06.022.
Wang J,Wu YJ,Wang Y,et al. Myocardial response to ischemia reperfusion injury in rats after X-ray irradiation[J].Chin J Radiat Oncol,2016,25(6):640-645.DOI:10.3760/cma.j.issn.1004-4221.2016.06.022.
[2] 武亚晶,王雪锋,王军,等.急性期放射性心肌损伤病理学表现及损伤机制研究[J].中华放射肿瘤学杂志,2016,25(10):1117-1122.DOI:10.3760/cma.j.issn.1004-4221.2016.10.021.
Wu YJ,Wang XF,Wang J,et al. Pathological manifestations of acute-stage radioactive myocardial damage and related mechanisms[J].Chin J Radiat Oncol,2016,25(10):1117-1122.DOI:10.3760/cma.j.issn.1004-4221.2016.10.021.
[3] 梁军,孙朝阳,张文正.食管癌低氧放疗的临床研究[J].中国现代医学杂志,2003,13(10):11-13.DOI:10.3969/j.issn.1005-8982.2003.10.004.
Liang J,Sun CY,Zhang WZ.A study on hypoxyradiotherapy for advanced esophageal cancer[J].China J Mod Med,2003,13(10):11-13.DOI:10.3969/j.issn.1005-8982.2003.10.004.
[4] 申良方,涂青松,魏瑞,等.鼻咽癌低氧放疗的前瞻性研究[J].中国现代医学杂志,2002,12(7):10-12.DOI:10.3969/j.issn.1005-8982.2002.07.004.
Shen LF,Tu QS,Wei R,et al. Prospective study on hypoxic radiotherapy of nasopharyngeal carcinoma[J].China J Mod Med,2002,12(7):10-12.DOI:10.3969/j.issn.1005-8982.2002.07.004.
[5] 孙衍伟,安永恒.低氧放疗对中晚期胰腺癌的放射防护作用[J].中华胰腺病杂志,2013,13(1):40-41.DOI:10.3760/cma.j.issn.1674-1935.2013.01.013.
Sun YW,An YH.Hypoxic radiotherapy for advanced pancreatic cancer in radiation protection[J]. Chin J Panc Dis,2013,13(1):40-41.DOI:10.3760/cma.j.issn.1674-1935.2013.01.013.
[6] Zhou JJ,Ma HJ,Liu Y,et al. The anti-arrhythmic effect of chronic intermittent hypobaric hypoxia in rats with metabolic syndrome induced with fructose[J].Can J Physiol Pharmacol,2015,93(4):227-232.DOI:10.1139/cjpp-2014-0343.
[7] Zhou JJ,Wei Y,Zhang L,et al. Chronic intermittent hypobaric hypoxia prevents cardiac dysfunction through enhancing antioxidation in fructose-fed rats[J].Can J Physiol Pharmacol,2013,91(5):332-337.DOI:10.1139/cjpp-2012-0059.
[8] Yuan F,Teng X,Guo Z,et al. Chronic intermittent hypobaric hypoxia ameliorates endoplasmic reticulum stress mediated liver damage induced by fructose in rats[J].Life Sci,2015,121:40-45.DOI:10.1016/j.lfs.2014.11.019.
[9] Boerma M.Experimental radiation-induced heart disease:past,present,and future[J].Radiat Res,2012,178(1):1-6.DOI:10.1667/RR2933.1
[10] Boerma M,Roberto KA,Hauer-Jensen M.Prevention and treatment of functional and structural radiation injury in the rat heart by pentoxifylline and alpha-tocopherol[J].Int J Radiat Oncol Biol Phys,2008,72(1):170-177.DOI:10.1016/j.ijrobp.2008.04.042.
[11] Mansour HH,Tawfik SS.Early treatment of radiation-induced heart damage in rats by caffeic acid phenethyl ester[J].Eur J Pharmacol,2012,692(1-3):46-51.DOI:10.1016/j.ejphar.2012.06.037.
[12] 张翼,杨黄恬,周兆年.间歇性低氧适应的心脏保护[J].生理学报,2007,59(5):601-613.DOI:10.3321/j.issn:0371-0874.2007.05.009.
Zhang Y,Yang HT,Zhou ZN.The cardioprotection of intermittent hypoxic adaptation[J].Acta Physiol Sinica,2007,59(5):601-613.DOI:10.3321/j.issn:0371-0874.2007.05.009.
[13] Zhang Y,Zhou ZN.Beneficial effects of intermittent hypobaric hypoxia on the body[J].Chin J Appl Physiol,2012,28(6):504-509.
[14] Poupa O,Krofta K,Prochzka J,et al. Acclimation to simulated high altitude and acute cardiac necrosis[J].Fed Proc,1966,25(4):1243-1246.
[15] Butt M,Dwivedi G,Khair O,et al. Obstructive sleep apnea and cardiovascular disease[J].Int J Cardiol,2010,139(1):7-16.DOI:10.1016/j.ijcard.2009.05.021.
[16] Zhang Y,Zhong N,Zhou ZN.Estradiol potentiates antiarrhythmic and antioxidative effects of intermittent hypoxic rat heart[J].Acta Pharmacol Sin,2000,21(7):609-612.
[17] Zhong N,Zhang Y,Zhu HF,et al. Myocardial capillary angiogenesis and coronary flow in ischemia tolerance rat by adaptation to intermittent high altitude hypoxia[J].Acta Pharmacol Sin,2002,23(4):305-310.
[18] Asemu G,Neckár,Szárszoi O,et al. Effects of adaptation to intermittent high altitude hypoxia on ischemic ventricular arrhythmias in rats[J].Physiol Res,2000,49(5):597-606.
[19] Zhang Y,Zhong N,Gia J,et al. Effects of chronic intermittent hypoxia on the hemodynamics of systemic circulation in rats[J].Jpn J Physiol,2004,54(2):171-174.DOI:10.2170/jjphysiol.54.171.