大鼠WBI后海马区NFATc4/3信号通路相关因子改变的研究

doi:10.3760/cma.j.issn.1004-4221.2017.01.018

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Abstract

Objective This study is to investigate the changes in the NFATc4/3 signaling pathway in rat hippocampus after whole brain radiation. Methods A total of 120 one-month-old male Sprague-Dawley rats were randomly divided into four groups to receive whole brain radiation using 4-MeV electron beams with doses of 0(control),2,10,and 20 Gy,respectively,in a single fraction. At 6 hours,12 hours,1 day,3 days,1 week,and 2 weeks after radiation,Western blot and real-time PCR were used to evaluate the changes in expression levels of CaN,NFATc4/3,p-NFATc4/3,and GSK-3β. Results There were no significant changes in the expression of NFATc4/3 or p-NFATc4/3 at 6 and 12 hours after whole brain radiation. At 1 day after radiation,compared with the control group,the expression of p-NFATc4/3 in the radiation groups was significantly increased in a dose-dependent manner (2 Gy:P=0.014;10 Gy:P=0.011;20 Gy:P=0.000);however,there was no significant difference in the expression of NFATc4/3 between the radiation group and the control group. The expression of NFATc4/3 was significantly decreased in the radiation groups than in the control group at day 3(2 Gy:P=0.040;10 Gy:P=0.000;20 Gy:P=0.000),1 week (2 Gy:P=0.692;10 Gy:P=0.032;20 Gy:P=0.021),and 2 weeks (2 Gy:P=0.001;10 Gy:P=0.000;20 Gy:P=0.000) after radiation,while there was no significant difference in the expression of p-NFATc4/3 between any two groups. There were no time-or dose-dependent changes in expression of CaN or GSK-3β. Conclusions Ionization radiation has an inhibitory effect on the NFATc4/3 signaling pathway in rat hippocampus. Combined with our previous results,this study suggests that radiation-induced cognitive dysfunction is associated with the NFATc4/3 signaling pathway.

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	Articles by authors
	Chen Qingqing
	Zhou Mengyun
	Sun Rui
	Zhang Qixian
	Cao Yi
	Tian Ye

Key words： Whole-brain irradiation NFATc4/3 gene Cognitive dysfunction

Received: 22 October 2015

Corresponding Authors: Tian Ye,Email:dryetian@126.com

Cite this article:

Chen Qingqing,Zhou Mengyun,Sun Rui et al. The change of NFATc4/3 signaling pathway in the rat hippocampus following whole brain irradiation[J]. Chinese Journal of Radiation Oncology, 2017, 26(1): 79-83.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2017.01.018 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2017/V26/I1/79

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