Abstract Objective To investigate the androgen-like effects of Cordyceps sinensis (CS) and its impact on the radiosensitivity of VCaP prostate cancer cells. Methods The hormone levels and weightindex of reproductive organs in mice were determined after gavage with CS. Clonogenic assay was performed to determine the impact of CS on the radiosensitivity of VCaP cells. The 3-(4, 5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium assay, flow cytometry, and tumor xenografts in nude mice were performed to determine the effects of CS on the proliferation of VCaP cells (androgen receptor-positive) and PC-3 cells (androgen receptor-negative) in vitro and in vivo. Serum prostate-specific antigen (PSA) levels in nude mice were evaluated. Data were analyzed by one-way analysis of variance or t-test. Results The testosterone level and weight of prostate in mice were significantly higher in the CS group than in the control group ((8.28±1.94) vs. (2.08±1.24) ng/ml, P=0.023;(0.53±0.04) vs. (0.31±0.04) mg/g, P=0.006). The radiosensitivity enhancement ratio (ratio of D0 values) was 0.80. The viability of VCaP cells was significantly higher in the CS group than in the control group (1.32±0.07 vs. 0.66±0.02, P=0.000), and colony forming efficiency was significantly enhanced in the CS group than in the control group (57.0%±1.9% vs. 47.0%±0.6%, P=0.005). VCaP tumor xenografts in nude mice were inclined to grow faster in the CS group than in the control group, and the serum PSA level in the CS group was significantly higher than that in the control group ((0.66±0.04) vs. (0.26±0.06) ng/ml, P=0.000). However, CS had no effect on PC-3 cells at the working concentration. Conclusions CS has the androgen-like effects. It may also promote the proliferation and reduce the radiosensitivity of androgen receptor-positive VCaP cells.
Ma Mingwei*,Gao Xianshu,Yu Hongliang et al. Androgen-like effects of Cordyceps sinensis and its impact on the radiosensitivity of VCaP prostate cancer cells[J]. Chinese Journal of Radiation Oncology, 2015, 24(3): 344-348.
Ma Mingwei*,Gao Xianshu,Yu Hongliang et al. Androgen-like effects of Cordyceps sinensis and its impact on the radiosensitivity of VCaP prostate cancer cells[J]. Chinese Journal of Radiation Oncology, 2015, 24(3): 344-348.
[1] Sim HG, Cheng CW. Changing demography of prostate cancer in Asia[J].Eur J Cancer,2005,41(6):834-845. [2] Vis AN, Schroder FH. Key targets of hormonal treatment of prostate cancer. Part 1:the androgen receptor and steroidogenic pathways[J]. BJU Int. 2009;104(4):438-48. [3] Zhu JS, Halpern GM,Jones K. The scientific rediscovery of an ancient Chinese herbal medicine:cordyceps sinensis:part I[J]. J Altern Complement Med,1998,4(3):289-303. [4] 戎耀方,包鸿俊,沈丽琳.虫草雄激素样作用的研究[J].南京农学院学报,1984,3(1):64-67. [5] Lee HH, Park C, Jeong JW, et al. Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen speciesmediated mitochondrial death pathway[J]. Int J Oncol,2013,42(3):1036-1044.DOI:10.3892/ijo.2013.1762. [6] Kuo YC, Lin CY, Tsai WJ, et al. Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides[J]. Cancer Invest,1994,12(6):611-615. [7] Yamada H, Kawaguchi N, Ohmori T, et al. Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides[J]. Carbohydr Res,1984,125(1):107-115. [8] Ohmori T,Tamura K,Fukui K, et al. Isolation of galactosaminoglycan moiety (CO-N) from protein-bound polysaccharide of Cordyceps ophioglossoides and its effects against murine tumors[J]. Chem Pharm Bull,1989,37(4):1019-1022. [9] Huang BM, Hsu CC, Tsai SJ, et al. Effects of Cordyceps sinensis on testosterone production in normal mouse Leydig cells[J]. Life Sci,2001,69(22):2593-2602. [10] Strauss JF 3rd, Kishida T, Christenson LK, et al. START domain proteins and the intracellular trafficking of cholesterol in steroidogenic cells[J]. Mol Cell Endocrinol,2003,202(1-2):59-65. [11] van Bokhoven A, Varella-Garcia M, Korch C, et al. Molecular characterization of human prostate carcinoma cell lines[J]. Prostate,2003,57(3):205-225. [12] Korenchuk S, MClean L, Lee YG,et al. VCaP, a cell-based model system of human prostate cancer.[J]. In Vivo,2001,15(2):163-168. [13] Feldman BJ, Feldman D. The development of androgen-independent prostate cancer[J]. Nature Reviews Cancer,2001,1(1):2. [14] Culig Z, Bartsch G. Androgen axis in prostate cancer[J]. J Cell Biochem,2006,99(2):373-381. [15] Park C, Kim Y, Shim M, et al. Hypoxia enhances ligand-occupied androgen receptor activity[J]. Biochem Biophys Res Commun,2012,418(2):319-323.DOI:10.1016/j.bbrc.2012.01.019. [16] Hobisch A, Eder IE, Putz T, et al. Interleukin-6 regulates prostate-specific protein expression in prostate carcinoma cells by activation of the androgen receptor[J]. Cancer Res,1998,58(20):4640-4645. [17] Balk SP, Knudsen KE.AR, the cell cycle, and prostate cancer[J/OL]. Nucl Recept Signal,2008,6:e001[2015-01-20].http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2254330/.DOI:10.1621/nrs.06001. [18] Agoulnik IU, Weigel NL.Androgen receptor action in hormone-dependent and recurrent prostate cancer[J]. J Cell Biochem,2006,99(2):362-372. [19] Cleutjens KB,van der Korput HA, van Eekelen CC, et al. An androgen response element in a far upstream enhancer region is essential for high, androgen-regulated activity of the prostate-specific antigen promoter[J]. Mol Endocrinol,1997,11(2):148-161.
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