沉默LncRNA OIP5-AS1通过上调miR-34c-5p表达增加A549R细胞放射敏感性

doi:10.3760/cma.j.issn.1004-4221.2020.01.013

Abstract
Figure/Table
References(11)
Related Citation (15)
Read Tracking
Download Tracking

Download: PDF (0 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS) Supporting Info

Abstract Objective To investigate the effect of LncRNA OIP5-AS1 on radiosensitivity of non-small cell lung cancer (NSCLC) cells and its mechanism. Methods The radiation-resistant cell A549R was established by using A549 cells irradiated by X-ray 6Gy in 5 fractions. The expression levels of OIP5-AS1 and miR-34c-5p in A549 and A549R cells were detected by qRT-PCR. OIP5-AS1 inhibitor or miR-34c-5p mimetic was transfected into A549R cells, or OIP5-AS1 overexpression plasmid was transfected into A549 cells. Cell apoptosis was detected by flow cytometry. Cell radiosensitivity was analyzed by colony formation assay. The expression levels of p-Chk2 and p-ATM proteins were measured by Western blot. Dual luciferase assay was adopted to verify the relationship between OIP5-AS1 and miR-34c-5p. Results Compared with A549 cells, the expression of OIP5-AS1 was significantly up-regulated in A549R cells (1.97±0.11 vs.1.01±0.05,P<0.05), whereas the expression of miR-34c-5p was remarkably down-regulated (0.43±0.02 vs.1.02±0.06,P<0.05). The expression levels of p-Chk2 and p-ATM proteins in A549R cells in the silencing OIP5-AS1+6Gy group were significantly lower (0.43±0.03 vs.1.39±0.15,0.51±0.0 5 vs.1.21± 0.11,both P<0.05), whereas the apoptotic rate was significantly higher than those in the silencing control+6Gy group [(13.29±1.25)% vs. (28.47±2.31)%,P<0.05)]. The expression levels of p-Chk2 and p-ATM proteins in A549 cells in overexpressing OIP5-AS1+6Gy group were significantly higher than those in overexpression control+6Gy group (1.23±0.13 vs.0.75±0.06,1.08±0.11 vs.0.59±0.04,both P<0.05). Inhibiting miR-34c-5p expression reversed the effect of silencing OIP5-AS1 on survival fraction of A549R cells (SER=1.42). OIP5-AS1 negatively regulated the expression of miR-34c-5p. Conclusion Silencing OIP5-AS1 enhances the radiosensitivity of radiation-resistant A549 cells by up-regulating the expression of miR-34c-5p, providing a potential target for radiotherapy of NSCLC cells.

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Mao Kai
	Ding Xiaohua
	Wu Liping
	Mao Yujing
	Zhang Liguo
	Li Jun
	Lu Jiang

Key words： A549 cell line A549R cell line OIP5-AS1 gene miR-34c-5p gene radiosensitivity

Received: 18 June 2019

Cite this article:

Mao Kai,Ding Xiaohua,Wu Liping et al. Silencing LncRNA OIP5-AS1 increases radiosensitivity of non-small cell lung cancer A549 cell line by up-regulating miR-34c-5p expression[J]. Chinese Journal of Radiation Oncology, 2020, 29(1): 57-60.

URL:

http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2020.01.013 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2020/V29/I1/57

[1]李鸣鹤,李守淼,张伟,等. 沉默长链非编码RNA HOTAIR对直肠腺癌细胞放射敏感性的影响[J]. 中华放射肿瘤学杂志,2018,27(12):1097-1100. DOI:10.3760/cma.j.issn.1004-4221.2018.12.015.
Li MH,Li SM,Zhang W,et al. Effect of silencing of long non-coding RNA HOTAIR on radiosensitivity of rectal adenocarcinoma cell lines[J]. Chin J Radiat Oncol, 2018,27(12):1097-1100. DOI:10.3760/cma.j.issn.1004-4221.2018.12.015.
[2]李玉椿. 长链非编码RNA H19对骨肉瘤顺铂化疗敏感性的影响及机制[J]. 中国老年学杂志,2017,37(24):6043-6046. DOI:10.3969/j.issn.1005-9202.2017.24.017.
Li YC. Effect and mechanism of long-chain non-coding RNA H19 on chemosensitivity of osteosarcoma to cisplatin[J]. Chin J Gerontol,2017,37(24):6043-6046. DOI:10.3969/j.issn.1005-9202.2017.24.017.
[3]Zou Y,Yao S,Chen X,et al. LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells[J]. Eur J Cell Biol,2018,97(5):369-378. DOI:10.1016/j.ejcb.2018.04.005.
[4]Wang M L,Sun X,Yang Y L,et al. Long non-coding RNA OIP5-AS1 promotes proliferation of lung cancer cells and leads to poor prognosis by targeting miR-378a-3p[J]. Thoracic Cancer,2018,9(8):939-949. DOI:10.1111/1759-7714.12767.
[5]孙燕,凌春华. 长链非编码RNA在非小细胞肺癌诊断、治疗中的应用进展[J]. 山东医药,2017,57(22):99-102. DOI:CNKI:SUN:SDYY.0.2017-22-037.
Sun Y,Ling CH. Progress in the application of long-chain non-coding RNA in the diagnosis and treatment of non-small cell lung cancer[J]. Shandong Med J,2017,57(22):99-102. DOI:CNKI:SUN:SDYY.0.2017-22-037.
[6]Wu D,Li Y,Zhang H,et al. Knockdown of LncRNA PVT1 enhances radiosensitivity in non-small cell lung cancer by sponging Mir-195.[J]. Cell Physiol Biochem,2017,42(6):2453-2466. DOI:10.1159/000480209.
[7]Liu XB,Zheng J,Xue YX,et al. PIWIL3/OIP5-AS1/miR-367-3p/CEBPA feedback loop regulates the biological behavior of glioma cells[J]. Theranostics,2018,8(4):1084-1105. DOI:10.7150/thno.21740.
[8]Deng J,Deng H,Liu C,et al. Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2[J]. Biomed Pharmacother,2018,98:102-110. DOI:10.1016/j.biopha.2017.12.031.
[9]刘敏. 微小RNA-34家族与肺部疾病的研究进展[J]. 国际儿科学杂志,2018,45(2):96-99. DOI:10.3760/cma.j.issn.1673-4408.2018.02.007.
Liu M. Effect of micro RNA-34 family on pulmonary diseases[J]. Int J Pediatr,2018,45(2):96-99. DOI:10.3760/cma.j.issn.1673-4408.2018.02.007.
[10]Duan W,Xu Y,Dong Y,et al. Ectopic expression of miR-34a enhances radiosensitivity of non-small cell lung cancer cells,partly by suppressing the LyGDI signaling pathway[J]. J Radiat Res,2013,54(4):611-619. DOI:10.1093/jrr/rrs136.
[11]Maki Y1,Asano H,Toyooka S,et al. MicroRNA miR-34b/c enhances cellular radiosensitivity of malignant pleural mesothelioma cells[J]. Anticancer Res,2012,32(11):4871-4876. DOI:10.0000/PMID23155254.