VPA-BSANPs对胶质瘤细胞系放射生物效应

doi:10.3760/cma.j.issn.1004-4221.2017.04.019

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Abstract Objective To investigate the radiobiological effects of VPA-BSANPs on C6 and U87 glioma cells in vitro. Methods C6 and U87 glioma cells were treated with different concentrations of VPA and VPA-BSANPs for 12 h and 24 h, and MTT assay was used to determine cell viability. C6 and U87 cells were treated with different concentrations of VPA and VPA-BSANPs combined with X-ray irradiation (0, 2, 4, 6, and 8 Gy), and colony formation assay was used to determine plating efficiency (PE). C6 and U87 glioma cells were treated with different concentrations of VPA and VPA-BSANPs for 12 h, followed by X-ray irradiation (0, 4, and 8 Gy), and flow cytometry using Annexin V-FITC/PI staining was used to examine cell apoptosis. Western blot was used to evaluate the effects of VPA and VPA-BSANPs on radiation-induced apoptosis protein expression. One-way ANOVA was used for comparison of means with homogeneity of variance between multiple groups, and the t-test was used for comparison of means between two groups. Results Without irradiation, VPA and VPA-BSANPs had no significant inhibitory effects on the proliferation of C6 and U87 cells (P=0.328,0.920).The PE of cells treated with VPA-BSANPs combined with irradiation was significantly lower than that of cells treated with VPA combined with irradiation (P=0.000). In C6 and U87 cells, VPA-BSANPs combined with irradiation increased the expression of p53 and Bax (P=0.000,0.000 and P=0.010,0.002), but reduced the expression of Bcl-2(P=0.008,0.000). Active caspase-3 fragments were only found in the cells treated with VPA-BSANPs combined with irradiation and VPA combined with irradiation, but were less in the former cells than in the latter cells (P=0.004). The active fragments of peroxisome proliferator-activated receptor were only found in the cells treated with VPA-BSANPs combined with irradiation. Conclusions VPA-BSANPs can increase the radiosensitivity of C6 and U87 glioma cells in vitro, possibly by promoting the apoptosis of tumor cells induced by radiation.

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	Articles by authors
	Guo Haijuan
	Zhang wei
	Zhao Xin
	Hou Huaying
	Xu Ying
	Jiang Yuhua

Key words： Histone deacetylase inhibitors Valproic acid Apoptosis

Received: 05 May 2016

Fund:Natural Science Foundation of Shandong Province (ZR2010HM080)

Corresponding Authors: Jiang Yuhua,Email:Email:jiangyuhua@sdu.edu.cn

Cite this article:

Guo Haijuan,Zhang wei,Zhao Xin et al. Radiobiological effects of VPA-BSANPs on C6 and U87 glioma cells[J]. Chinese Journal of Radiation Oncology, 2017, 26(4): 459-462.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2017.04.019 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2017/V26/I4/459

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