AbstractObjective To explore the relationship between HLA-B allele polymorphisms and nasopharyngeal carcinoma (NPC) in Xinjiang, China and its clinical significance. Methods A total of 226 patients were assigned to NPC group, while 207 healthy volunteers were assigned to control group. PCR amplification with sequence-specific primers was used to determine HLA-B alleles. Comparison of HLA-B allele frequency between the above two groups, between Han and Uygur populations, and between patients with various clinical characteristics of NPC was made by chi-square test. The Kaplan-Meier method was used to calculate the survival rates and the log-rank univariate analysis was used to explore the relationship between survival rates and HLA-B allele frequency. Results In all the subjects or Han population alone, the allele frequency of HLA-B*46 in the NPC group was significantly higher than that in the control group (P=0.000;P=0.000). In Uygur population, however, there was no significant difference in the allele frequency of HLA-B*46 between the NPC group and the control group (P>0.05). In the patients with NPC, those less than 30 years old had a significantly higher allele frequency of HLA-B*44 than those no less than 30 years old (P=0.029);those with differentiated non-keratinizing carcinoma had a significantly higher allele frequency of HLA-B*48 than those with undifferentiated non-keratinizing carcinoma (P=0.029);those with stage T1+T2 disease had a significantly higher allele frequency of HLA-B*48 than those with stage T3+T4 disease (P=0.029). The 5-year overall survival, disease-free survival, distant metastasis-free survival, and local relapse-free survival rates had no relationship with the expression of HLA-B*46, HLA-B*44, or HLA-B*48 in NPC patients (all P>0.05). Conclusions HLA-B*46 allele is probably a NPC susceptibility gene in Han population in Xinjiang. HLA-B*44 is probably associated with early age of onset, while HLA-B*48 is probably associated with the pathological type and T stage of NPC. Therefore, HLA-B alleles are probably associated with the development and progression of NPC.
Fund:International Cooperation Project of Ministry of Science and Technology (2012DFA31560);Achievement Promotion Project of Xinjiang Uygur Autonomous Region (201554142);Key Laboratory Project of Xinjiang Uygur Autonomous Region (2015KL021)
Corresponding Authors:
Wang Ruozheng,Email:wrz8526@163.com
E-mail: wrz8526@163.com
Cite this article:
Hu Yunhui,Liu Kai,Geng Xiaotao et al. HLA-B alleles and nasopharyngeal carcinoma in Xinjiang, China:relationship and clinical significance[J]. Chinese Journal of Radiation Oncology, 2016, 25(8): 795-801.
Hu Yunhui,Liu Kai,Geng Xiaotao et al. HLA-B alleles and nasopharyngeal carcinoma in Xinjiang, China:relationship and clinical significance[J]. Chinese Journal of Radiation Oncology, 2016, 25(8): 795-801.
[1] Tsao SW,Yip YL,Tsang CM,et al. Etiological factors of nasopharyngeal carcinoma[J].Oral Oncol,2014,50(5):330-338.DOI:10.1016/j.oraloncology.2014.02.006 [2] Hassen E,Nahla G,Bouaouina N,et al. The human leukocyte antigen class I genes in nasopharyngeal carcinoma risk[J].Mol Biol Rep, 2010,37(1):119-126.DOI:10.1007/s11033-009-9548-9 [3] Tang MZ,Cai YL,Zheng YM,et al. Association between human leukocyte antigen and nasopharyngeal-carcinoma[J].Hereditas,2012,34(12):1505-1512 [4] Wang R,Hu Y,Yindom LM,et al. Association analysis between HLA-A,-B,-C,-DRB1, and-DQB1 with nasopharyngeal carcinoma among a Han population in Northwestern China[J].Hum Immunol,2014,75(3):197-202.DOI:10.1016/j.humimm.2013.12.015 [5] Goldsmith DB,West TM,Morton R.HLA associations with nasopharyngeal carcinoma in Southern Chinese:a meta-analysis[J].Clin Otolaryngol Allied Sci,2002,27(1):61-67.DOI:http://dx.doi.org/10.1046/j.0307-7772.2001.00529.x [6] Burt RD,Vaughan TL,McKnight B,et al. Associations between human leukocyte antigen type and nasopharyngeal carcinoma in Caucasians in the United States[J].Cancer Epidemiol Biomarkers Prev,1996,5(11):879-887 [7] Shen CM,Zhu BF,Deng YJ,et al. Allele polymorphism and haplotype diversity of HLA-A,-B and-DRB1 loci in sequence-based typing for Chinese Uyghur ethnic group[J/OL].PLoS One,2010,5(11):e13458.DOI:10.1371/journal.pone.0013458 [8] Shuxian J,Xiaoyun C,Zhihui F,et al. Association of HLA-B*51:01 with papillary thyroid carcinoma in the Chinese Han population of the Shandong coastal areas[J].Thyroid,2014,24(5):867-871.DOI:10.1089/thy.2013.0130 [9] Qiu X,Zhang F,Chen D,et al. HLA-B*07 is a high risk allele for familial cervical cancer[J].Asian Pac J Cancer Prev,2011,12(10):2597-2600 [10] Yang L,Wang LJ,Shi GL,et al. Analysis of HLA-A, HLA-B and HLA-DRB1 alleles in Chinese patients with lung cancer[J].Genet Mol Res,2010,9(2):750-755.DOI:10.4238/vol9-2gmr735 [11] Wang SS,Abdou AM,Morton LM,et al. Human leukocyte antigen class Ⅰ and Ⅱ alleles in non-Hodgkin lymphoma etiology[J].Blood,2010,115(23):4820-4823.DOI:10.1182/blood-2010-01-266775 [12] Bulut I,Meral M,Kaynar H,et al. Analysis of HLA class I and Ⅱ alleles regarding to lymph node and distant metastasis in patients with non-small cell lung cancer[J].Lung Cancer,2009,66(2):231-236.DOI:10.1016/j.lungcan.2009.01.012 [13] Lu Y,Abdou AM,Cerhan JR,et al. Human leukocyte antigen class Ⅰand Ⅱ alleles and overall survival in diffuse large B-cell lymphoma and follicular lymphoma[J].Scientific World J,2011,11:2062-2070.DOI:10.1100/2011/373876 [14] Nagata Y,Hanagiri T,Mizukami M,et al. Clinical significance of HLA class I alleles on postoperative prognosis of lung cancer patients in Japan[J].Lung Cancer,2009,65(1):91-97.DOI:10.1016/j.lungcan.2008.10.012
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