AbstractObjective To explore the effects of different courses of electro-acupuncture at Baihui, Fengfu, and Shenshu of both sides on the behavior of mice exposed to radiation. Methods Fifty C57BL/6J mice 30 days old were randomly and equally divided into control group, model group (exposure to radiation of 8 Gy), electro-acupuncture group 1(1 week of treatment), electro-acupuncture group 2(2 weeks of treatment), and electro-acupuncture group 3(3 weeks of treatment). The mouse model of radiation-induced brain injury was established by exposure to radiation of 8 Gy and treated with electro-acupuncture. The open field test and Morris water maze test were used to evaluate the cognitive function and spatial learning and memory, respectively. Group t-test difference. Results In the open field test, the model group had significantly fewer vertical or horizontal movements than the control group (P=0.032,0.029,0.028), while the electro-acupuncture groups 2 and 3 had significantly more vertical or horizontal movements than the model group (P=0.001,0.017,0.000). In the Morris water maze test, compared with the control group, the model group had significantly longer latency, a significantly lower frequency of entry into the goal box, and significantly shortened residence time, indicating substantially worse performance of spatial learning and memory (P=0.023,0.001,0.001).Compared with the model group, the electro-acupuncture groups 2 and 3 had significantly shortened latency, a significantly higher frequency of entry into the goal box, andsignificantly longer residence time (P=0.046,0.017,0.049,0.007,0.031,0.009). Conclusions Different courses of electro-acupuncture treatment at Baihui, Fengfu, and Shenshu of both sides can substantially improve the cognitive function and spatial learning and memory in mice exposed to radiation. Particularly, 2 and 3 weeks of electro-acupuncture treatment give the best outcomes.
Wu Xin,Zheng Wanjun,Li Lin et al. Experimental study on different courses of electro-acupuncture treatment on behavior of model mice with radiographic exposure[J]. Chinese Journal of Radiation Oncology, 2016, 25(6): 634-637.
Wu Xin,Zheng Wanjun,Li Lin et al. Experimental study on different courses of electro-acupuncture treatment on behavior of model mice with radiographic exposure[J]. Chinese Journal of Radiation Oncology, 2016, 25(6): 634-637.
[1] 郭韬,刘斌,郑鑫.放射性脑病研究进展[J].脑与神经疾病杂志,2009,16(5):649-651.DOI:1006-351X (2008)05-0684-04. Gu T,Liu B,Zheng X.The study on the radiation encephalopathy[J].J Brain Ner Dis,2009,16(5):649-651.DOI:1006-351X (2008)05-0684-04. [2] Gao JF,Wang S,Wang XY,et al. Electroacupuncture enhances cell proliferation and neuronal differentiation in young rat brains[J].Neurol Sci.2011,32(3):369-374.DOI:10.1007/s10072-010-040206. [3] Sestakova N,Puzserova A,Kluknavsky M,et al. Determination of motor activity and anxiety-related behavior in rodents:methodological aspects and role of nitric oxide[J].Interdiscip Toxicol,2013,6(3):126-135.DOI:10.2478/intox-2013-0020. [4] 范兴文,贯十阔,吴开良.脑部照射对大鼠情绪和记忆的影响[J].中国癌症杂志,2014,24(11):814-819.DOI:10.3969/j.issn:1007-3969.2014.11.003. Fan XW,Guang SHK,Wu KL.The effect of brain irradiation on mood and memory for rats[J].China Oncol,2014,24(11):814-819.DOI:10.3969/j.issn:1007-3969.2014.11.003. [5] McLachlan RS.A brief review of the anatomy and physiology of the limbic system[J].Can J Neurol Sci,2009,36 Suppl 2:S84-87. [6] Gruart A,Munz MD,Delgado-Garcia JM.Involvement of the CA3-CA1 synapse in the acquisition of associative learning in behaving mice[J].J Neurosi,2006,26(4):1077-1086.DOI:10.1523/JNEUROSCI.2834-05.2006. [7] Parihar VK,Limoli CL.Cranial irradiation compromises neuronal architecture in the hippocampus[J].Proc Natl Acad Sci USA,2013,110(31):12822-12827.DOI:10.1073/pnas.1307301110. [8] Roughton K,Bostrom M,Kalm M,et al. Irradiation to the young mouse brain impaired white matter growth more in the females than in the males[J].Cell Death Dis.2013,4(10):e897.DOI:1038/cddis.2013.423. [9] Zhu CL,Gao JF,Li Q,et al. Repeated exposure of the developing rat brain to magnetic resonance imaging did not affect neurogenesis,cell death or memory function[J].Biochem Biophys Res Commun,2011,404(1):291-296.DOI:10.1016/j.bbrc.2010.11.109. [10] Cao Y,Tsien Cl,Sundgren PC,et al. Dynamic contrast-enhanced magnetic resonance imaging as a biomarker for prediction of radiation-induced neurocognitive dysfunction[J].Clin Cancer Res,2009,15(5):1747-1754.DOI:10.1158/1078-0432.CCR-08-1420. [11] Jenrow KA,Brown SL,Lapanowski K,et al. Selective inhibition of microglia-mediated neuroinflammation mitigates radiation-induced cognitive impairment[J].Radiat Res,2013,179(5):549-556.DOI:10.1667/RR3026.1. [12] Collins-underwood JR,Zhao W,Sharpe JG,et al. NADPH oxidase mediates radiation-induced oxidative stress in rat brain microvascular endothelial cells[J].Free Radic Biol Med,2008,45(6):929-938.DOI:10.1016/j.freeradbiomed.2008.06.024. [13] 朱广迎,梁克,欧广飞,等.全脑照射后昆明鼠的学习、记忆力损伤记起变化规律[J].中华精神科杂志,2011,34(1):38-41.DOI:10.3760/j.issn:1006-7884.2001.01.011. Zhu GY,Liang K,Ou GF,et al. Dynamic changing of mouse learning and memory ability after whole brain irradiation[J]. Chin J Psychiat, 2011,34(1):38-41.DOI:10.3760/j.issn:1006-7884.2001.01.011. [14] Pokorny J,Martan T,Foletti A.High capacity optical channels for bioinformation transfer:acupuncture meridian[J].J Acupunc Meridian Stud,2012,5(1):34-41.DOI:10.1016/j.jams.2011.11.017. [15] Braga FP,Lemos Junior CA,Alves FA,et al. Acupuncture for the prevention of radiation-induced xerostomia in patients with head and neck cancer[J].Braz Oral Res,2013,25(2):180-185.DOI:10.1590/S1806-83242011000200014.
2016, Vol. 25 
