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A longitudinal study of transcriptional profiling of carbon-ions exposure on the lung
Zhou Cheng1, Wen Lei2, Su Shengfa3, Lu Shun4, Xu Zhiyuan5, Cheng Hao6, Shan Changguo2, Lai Mingyao2, Cai Linbo2, Chen Longhua1, Chen Ming7, Zhou Zhaoming2
1Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2Department of Oncology, Guangdong Sanjiu Brain Hospital, Guangzhou 510510, China; 3Department of Oncology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; 4Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, University of Electronic Science and Technology of China, Chengdu 610041, China; 5Department of Radiation Oncology, the University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China; 6Department of Radiation Oncology, Chenzhou First People′s Hospital, Chenzhou 423002, China; 7Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China
AbstractObjective To investigate the expression changes at the transcriptional level in normal lung tissues of mice after exposure to heavy ion radiation for different durations at different doses, aiming to provide evidence for exploring sensitive genes of heavy ion radiation, heavy ion radiation effect and the damage mechanism. Methods Experiments on the temporal kinetics:the whole thorax of mice was irradiated with 14.5Gy carbon-ions and the total RNA of lung tissue was extracted at 3days, 7days, 3 weeks and 24 weeks. In dose-dependent experiment, the total RNA of lung tissue was extracted at 1 week after irradiated with a growing thoracic dose of 0, 7.5, 10.5, 12.5, 14.5, 17.5 and 20Gy. Protein-to-protein interaction (PPI) analysis and gene-ontology biological process enrichment analysis were performed on significant differentially expressed genes (DEGs). Results A clearly differential expression patterns were observed at 3-day (acute stage), 1-week (subacute stage), 3-week (inflammatory stage) and 24-week (fibrosis stage) following 14.5Gy carbon-ions irradiation. Among those, the 3-day time point was found to be the mostly different from the other time points, whereas the 7-day time point had the highest uniformity with the other time points. Cellular apoptosis was the main type of cell death in normal lung tissues following carbon-ions exposure. The interactive genes of Phlda3, GDF15, Mgmt and Bax were identified as the radiosensitive genes, and Phlda3 was the center (R=0.76, P<0.001). Conclusion The findings in this study provide transcriptional insights into the biological mechanism underlying normal lung tissue toxicity induced by carbon-ions.
Fund:Basic and Applied Fundamental Research Foundation of Guangdong Province (2019A1515011943), National Key Research & Development Program of China (2017YFC0113200), Science and Technology Program of Guangzhou (202002030445,202002030086)
Zhou Cheng,Wen Lei,Su Shengfa et al. A longitudinal study of transcriptional profiling of carbon-ions exposure on the lung[J]. Chinese Journal of Radiation Oncology, 2021, 30(7): 721-727.
Zhou Cheng,Wen Lei,Su Shengfa et al. A longitudinal study of transcriptional profiling of carbon-ions exposure on the lung[J]. Chinese Journal of Radiation Oncology, 2021, 30(7): 721-727.
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2021, Vol. 30 
