VPA-BSANPs对胶质瘤细胞系放射生物效应

doi:10.3760/cma.j.issn.1004-4221.2017.04.019

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摘要目的研究纳米VPA-BSANPs复合物对C6、U87胶质瘤细胞系的体外放射生物效应。方法 C6、U87细胞分别经不同浓度VPA、VPA-BSANPs作用12、24 h后MTT法检测群体细胞存活率,经不同浓度VPA、VPA-BSANPs联合X线0、2、4、6、8 Gy照射后克隆形成实验检测PE值,经不同浓度VPA、VPA-BSANPs作用12 h后X线0、4、8 Gy照射应用Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡情况。应用蛋白免疫印迹方法检测VPA、VPA-BSANPs对射线诱导细胞凋亡蛋白表达影响。多组均数检验方差齐性后用单因素方差分析,两样本均数间进行t检验。结果 VPA、VPA-BSANPs对C6、U87胶质瘤细胞并无明显增殖抑制作用(P=0.328、0.920);VPA-BSANP联合照射的PE值明显低于VPA联合照射(P=0.000)。C6、U87细胞VPA-BSANPs联合照射的p53、Bax表达增加(C6细胞P=0.000、0.000和U87细胞P=0.010、0.002),Bcl-2表达降低(C6细胞P=0.008、U87细胞P=0.000)。Caspase-3激活片段仅见于VPA-BSANPs+照射、VPA+照射,且前者低于后者(P=0.004)。PPAR激活片段仅见于VPA-BSANPs+照射。结论 VPA-BSANPs可增加C6、U87胶质瘤细胞系放射生物效应,其机制与定向促进X线诱导的肿瘤细胞凋亡有关。

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	郭海娟
	张伟
	赵昕
	侯华英
	徐英
	姜玉华

关键词 ：组蛋白去乙酰化酶抑制剂, 丙戊酸钠, 细胞凋亡

Abstract：Objective To investigate the radiobiological effects of VPA-BSANPs on C6 and U87 glioma cells in vitro. Methods C6 and U87 glioma cells were treated with different concentrations of VPA and VPA-BSANPs for 12 h and 24 h, and MTT assay was used to determine cell viability. C6 and U87 cells were treated with different concentrations of VPA and VPA-BSANPs combined with X-ray irradiation (0, 2, 4, 6, and 8 Gy), and colony formation assay was used to determine plating efficiency (PE). C6 and U87 glioma cells were treated with different concentrations of VPA and VPA-BSANPs for 12 h, followed by X-ray irradiation (0, 4, and 8 Gy), and flow cytometry using Annexin V-FITC/PI staining was used to examine cell apoptosis. Western blot was used to evaluate the effects of VPA and VPA-BSANPs on radiation-induced apoptosis protein expression. One-way ANOVA was used for comparison of means with homogeneity of variance between multiple groups, and the t-test was used for comparison of means between two groups. Results Without irradiation, VPA and VPA-BSANPs had no significant inhibitory effects on the proliferation of C6 and U87 cells (P=0.328,0.920).The PE of cells treated with VPA-BSANPs combined with irradiation was significantly lower than that of cells treated with VPA combined with irradiation (P=0.000). In C6 and U87 cells, VPA-BSANPs combined with irradiation increased the expression of p53 and Bax (P=0.000,0.000 and P=0.010,0.002), but reduced the expression of Bcl-2(P=0.008,0.000). Active caspase-3 fragments were only found in the cells treated with VPA-BSANPs combined with irradiation and VPA combined with irradiation, but were less in the former cells than in the latter cells (P=0.004). The active fragments of peroxisome proliferator-activated receptor were only found in the cells treated with VPA-BSANPs combined with irradiation. Conclusions VPA-BSANPs can increase the radiosensitivity of C6 and U87 glioma cells in vitro, possibly by promoting the apoptosis of tumor cells induced by radiation.

Key words： Histone deacetylase inhibitors Valproic acid Apoptosis

收稿日期: 2016-05-05

基金资助:山东省自然科学基金(ZR2010HM080)

通讯作者: 姜玉华,Email:jiangyuhua@sdu.edu.cn

引用本文:

郭海娟,张伟,赵昕等. VPA-BSANPs对胶质瘤细胞系放射生物效应[J]. 中华放射肿瘤学杂志, 2017, 26(4): 459-462.

Guo Haijuan,Zhang wei,Zhao Xin et al. Radiobiological effects of VPA-BSANPs on C6 and U87 glioma cells[J]. Chinese Journal of Radiation Oncology, 2017, 26(4): 459-462.

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http://journal12.magtechjournal.com/Jweb_fszlx/CN/10.3760/cma.j.issn.1004-4221.2017.04.019 或 http://journal12.magtechjournal.com/Jweb_fszlx/CN/Y2017/V26/I4/459

[1] Hess-Stumpp H.Histone deacetylase inhibitors and cancer:from cell biology to the clinic[J].Eur J Cell Biol,2005,84(2-3):109-121.DOI:10.1016/j.ejcb.2004.12.010
[2] Kostrouchová M,Kostrouch Z,Kostrouchová M.Valproic acid,a molecular lead to multiple regulatory pathways[J].Folia Biol,2007,53(2):37-49
[3] Debeb BG,Xu W,Mok H,et al. Differential radiosensitizing effect of valproic acid in differentiation versus self-renewal promoting culture conditions[J].Int J Radiat Oncol Biol Phys,2010,76(3):889-895.DOI:10.1016/j.ijrobp.2009.09.052
[4] Luo Z,Ding XW,Hu Y,et al. Engineering a hollow nanocontainer platform with multifunctional molecular machines for tumor-targeted therapy in vitro and in vivo[J].ACS Nano,2013,7(11):10271-10284.DOI:10.1021/nn404676w
[5] Kakuta T,Takashima Y,Nakahata M,et al. Preorganized hydrogel:self-healing properties of supramolecular hydrogels formed by polymerization of host-guest-monomers that contain cyclodextrins and hydrophobic guest groups[J].Adv Mater,2013,25(20):2849-2853.DOI:10.1002/adma.201205321
[6] Peng LL,Peng X,Liu BR,et al. Ultrathin two-dimensional MnO2/graphene hybrid nanostructures for high-performance,flexible planar supercapacitors[J].Nano Lett,2013,13(5):2151-2157.DOI:10.1021/nl400600x
[7] Taratula O,Kuzmov A,Shah M,et al. Nanostructured lipid carriers as multifunctional nanomedicine platform for pulmonary co-delivery of anticancer drugs and siRNA[J].J Control Release,2013,171(3):349-357.DOI:10.1016/j.jconrel.2013.04.018
[8] Park S,Kang S,Chen XY,et al. Tumor suppression via paclitaxel-loaded drug carriers that target inflammation marker upregulated in tumor vasculature and macrophages[J].Biomaterials,2013,34(2):598-605.DOI:10.1016/j.biomaterials.2012.10.004
[9] Schroeder JE,Shweky I,Shmeeda H,et al. Folate-mediated tumor cell uptake of quantum dots entrapped in lipid nanoparticles[J].J Control Release,2007,124(1-2):28-34.DOI:10.1016/j.jconrel.2007.08.028.
[10] 吴海涛,占益平,曲彦明,等.叶酸受体α在人脑胶质瘤组织中表达及其临床意义[J].中华外科杂志,2014,52(3):202-207.DOI:10.3760/cma.j.issn.0529-5815.2014.03.011.
Wu HT,Zhan YP,Qu YM,et al. Changes of folate receptor-a protein expression in human gliomas and its clinical relevance[J].Chin J Surg,2014,52(3):202-207.DOI:10.3760/cma.j.issn.0529-5815.2014.03.011.
[11] Zhang HL,Jiang WH,Zhao SG,et al. Neuronal selective targeting,protection and signaling network analysis via dopamine-mediated mesoporous silica nanoparticles[J].Med Chem Comm,2015,6(7):1117-1129.
[12] Chinnaiyan P,Cerna D,Burgan WE,et al. Postradiation sensitization of the histone deacetylase inhibitor valproic acid[J].Clin Cancer Res,2008,14(17):5410-5415.DOI:10.1158/1078-0432.CCR-08-0643.
[13] 牛俊婕,王晗,徐英,等.丙戊酸钠增强大鼠胶质瘤C6细胞放射敏感性的体外实验[J].山东大学学报(医学版),2013,51(6):15-19.DOI:10.6040/j.issn.1671-7554.2013.06.004.
Niu JJ,Wang H,Xu Y,et al. Sodium valproate enhances in vitro radiosensitivity of C6 cell glioma in rats[J].J Shandong Univ (Health Sci),2013,51(6):15-19.DOI:10.6040/j.issn.1671-7554.2013.06.004.